非抗原性的

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01)；若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%，特异性为94.3%；4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05)，且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异（P＜0.01）；若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%，特异性为94.3%；4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验（P＜0.05，且大于各单个抗体检测敏感性。

As the exosomes are derived from tumor cells, carry tumor antigens and share characters with most of the vaccines prepared previously, they are a kind of noncellular novel vaccine.

由于这种EXOSOME来自于肿瘤细胞，携带有肿瘤抗原，又有几乎所有目前研制的高效肿瘤疫苗所必须的公刺激信号的特性，强烈提示这是一种非细胞性的新型疫苗。

Recombinatino polypeptide or chemically synthesized polypeptide antigen is used to immunize the mammal to obtain polyclonal antibody and the nonconservative amion acid sequence of the antiboby to N-terminal with recognition site qBrn-2 of development regulatory protein qBrn-2. The antibody does not recognize other POU structure domain protein, and recognizes specifically qunique protein in Western imprinting experiment. The qBrn-2 expression mode obtained immunohistochemistry process is identical with the resulted height of in-situ molecular hydridization.

以重组多肽或化学合成多肽为抗原免疫哺乳动物获得的多克隆抗体，这一抗体对发育调控蛋白qBrn－2的识别位点为qBrn－2的N－末端的非保守性的氨基酸序列，该抗体不识别其它POU结构域蛋白；在Western印记实验中，该抗体特异地识别单一的蛋白；利用免疫组织化学接示的qBrn－2表达模式与分子原位杂交的结果高度一致。

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达，包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性，以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

ObjectiveTo investigate the therapeutic effect of Maxingshigan Composition on Bronchial Asthma.MethodsAdopt phosphonic acid and histamine to evoke cobaya asthma.

支气管哮喘是机体对抗原性或非抗原性刺激引起的一种气管-支气管反应性过度增高的疾病，简称哮喘，已成为严重威胁人类健康的疾病之一。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

The nuclear protein encoded by N gene is consist of 422 AA residues (47kDa), Each virion contains 1258 copies that can effectively protect viral RNA from being digested by nucleotidase. The N protein presents a group specificity, it exists in various types and subtypes of VS and plays a very important role in transcript copy. Due to its characteristics of high antigenicity, the N protein can stimulate the body producing nonneutralized antibody.

它可有效的保护病毒RNA免受各种核酸酶的消化。N基因编码的N蛋白呈群特异性，为许多型和亚型所共有，有高的抗原性，刺激机体产生非中和抗体，且在转录复制中担任了重要的角色。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。