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靛玉红

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Objective:To establish a method for extracting of measuring the content of indirubin in banlangen granules by RP-HPLC,in order to provide theoretical basis for the quality standards of banlangen granules.

小 目的:建立板蓝根颗粒中靛玉红的含量测定方法,为板蓝根颗粒的质量标准控制提供理论依据。

The HPLC method is simple, precise and suitable for the content determination of indirubin in SFE material of Daqingye.

HPLC法操作简便,结果准确,可作为超临界萃取大青叶中靛玉红的质量控制方法。

An HPLC method for identifying indigo and indirubin was proposed to indicate the Daqingye in Kuhuang injection.

同时建立了苦黄注射液中大青叶药材主要成分靛蓝和靛玉红的HPLC鉴别方法。

Phytosome and SEDDS are in favor of improving oral bioavailability of indirubin.

磷脂复合物和自乳化释药系统有利于改善靛玉红口服生物利用度。

METHODS: Content of indirubin was used as index to evaluate the technologies based on orthogonal design, in which 4 factors considered were extraction temperature and pressure, separation temperature and pressure.

采用超临界萃取法进行L9(34)正交实验。以靛玉红含量考察萃取温度、萃取压力、分离温度及分离压力四个因素的影响。

The MTT method was used to investigate the effects of indirubin on the cell growth of K562,carcinoma of gallbladder ,hilar cholangiocarcinoma.

用MTT方法观测靛玉红对K562、胆囊癌、肝门胆管癌细胞株的影响。

Indirubin has been used clinically to treat chronic granulocytic leukemia, but poor solubility limited its absorption in the body.

靛玉红用于临床治疗慢性粒细胞白血病已有多年',疗效明确,但溶解度差,影响在体内的吸收。

Objective: There are three pathways for indirubin, an anti-tumor component of Chinese traditional medicine, to inhibit the growth of cancer cells. The three pathways are competitively inhibit CDKs with the kinase's ATP-binding pocket, directly inhibits c-Src kinase and block constitutive Stat3 signaling, and inhibition of nucleoside transporters respectively.

目的:中药抗肿瘤成份靛玉红可通过3种途径对肿瘤细胞的生长发挥抑制作用,分别为竞争性拮抗CDK激酶的ATP结合位点;抑制Src激酶、阻断Stat3信号通路;抑制核苷酸转运蛋白。

The HPLC method showed that the average recovery of indirubin and cholic acid was 100.3% with RSD=2.1% and 98.9% with RSD=1.8% respectively.

含量测定靛玉红平均回收率为100.3%,RSD=2.1%;胆酸平均回收率为98.9%,RSD=1.8%。

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