青霉菌

Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫链丝菌素抗性基因的pIJ903的单—BamHI位点，同向插入了FR-008生物合成基因簇两个最远端的4.0kb和1.5kb片段，并在二者之间插入了可在链霉菌FR-008和变铅青链霉菌中表达的阿泊拉霉素抗性基因，同时也插入了有助于将质粒引入链霉菌的oriT片段，从而构建出了置换整个基因簇的基因置换质粒pHZ691。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索，从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因，构建了doxA基因的链霉菌表达质粒，导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌，并研究了doxA基因在工程菌中的表达，最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

Through the experiments of the seedbed cultivation, the influence of rice seedling growth characteristic and phosphorus absorption on the low nutrient soil of available phosphorus that using sturdy-seeding agent which inoculates penicillium of dissolving phosphorus.

通过苗床培养试验，研究了在低有效磷营养土上施用接种解磷青霉菌的壮秧剂对水稻秧苗的生长特性及磷素吸收的影响。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Could be used as an efficient biosorbent for removal of heavy metal ions from aqueous solution and that the process was strongly affected by pH.

研究发现，溶液pH值对吸附过程有较大影响，最佳吸附pH值在5.5附近；最佳吸附温度为25～35℃；溶液浓度在10～500mg·L^-1范围内，固定化简青霉菌对重金属的吸附随金属离子浓度的增加而增加；吸附过程符合Langmuir等温吸附模型；生物吸附平衡时间约为60min。

Objective Observe the effect of temperature,pH and their interaction on Penicillium citreoviridin's toxin production in laboratory.

择要： 目的在尝试室前提下不雅察看温度、pH对黄绿青霉菌产毒的影响及其交互浸染。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp，不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因，但是pPR2.3c基因编码了一个双结构域蛋白，分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段，将几乎覆盖全长pPR2的两段DNA进行克隆后，不能转化变铅青链霉菌。

Fungus cultured: yeast like cells were found in the culture at 37℃ or in tissues.

真菌培养：在37℃培养或组织中马尔尼菲青霉菌呈酵母型，组织细胞外真菌长，弯曲，有横膈的腊肠状；在25℃培养中呈菌丝型，并产生红色色素扩散入培养基中。

The isolate Paecilomyces farinosus sw03032 was cultured in a same liquid medium at 25℃ and the impact of rotate speed of rocking bed,initial inoculum and initial pH value on mycelial bioass were studied.

在25℃下同一种培养液中，研究了摇床转速、接种量和初始pH值对粉拟青霉菌丝生长量的影响。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失