青酶

Fruit of Angeleno plum cultivar were used as material for studying the changes and relationship of the content of anthocyanin, endogenous hormone, PAL and PPO activity in the skin of Angeleno plum fruit.

为了明确李果实花青苷合成的调控因子，以安哥诺李为试材，对果皮花青苷、内源激素和PAL、PPO活性的变化以及内源激素、酶活性与花青苷的相关性进行研究。

Most bacteria were resistant to βlactam antibiotics, but sensitive to aminoglycosides, Carbopenem antibiotics and quinolones antibiotics.

细菌多对β内酰胺类抗生素耐药，普遍对氨基糖苷类、碳青酶烯类和喹诺酮类抗生素敏感。

The characteristic of pectinases production by solid state fermentation with Penicillium oxalicum BZH-2002 and lixiviation condition were investigated.

对草酸青霉菌BZH-2002菌株固体发酵果胶酶的特性及浸提条件进行了研究，确定其最佳产酶时期为72-92h，利用干物质量最快的时期为24-72h，发酵产酶期间培养基介质中pH值呈"V"字形变化。

Results The percentage of produced metallo-β-lactamase, lossed outer membrane OprinD2 and active efflux system in P A resistance to carbapenem are 29.8%, 31.9% and 31.9%, produced other β-lactamase is 6.4%, Conclusion Metallo-β-lactamase production is one of the main mechanisms in PA resistance to carbapinem, the result of correct detection in laboratory can not only promote the rational application of antimicrobial agents but also prevent the spread of bacterial resistance

结果 产金属酶、D2缺失、外排泵出分别占29.8%、31.9%、31.9%，产其他β-内酰胺酶占6.4%。结论产金属酶是PA耐碳青酶烯类抗菌药物最主要机制之一，准确的实验室检测不仅可以帮助临床合理选用抗菌药物并可减少细菌耐药性的传播。

Assulta and the induction of GST activity by nicotine and capsicine in H. assulta provided partial supports for the hypothesis that diet breadth was governed by detoxification abilities. The results also indicated that two sibling species had different strategies of adaptation to toxic substances: H. assulta saves expenditure of nutrients and energy by restricting their countermeasures to a few potentially toxic compounds. H. armigera, by contrast, must be prepared to counter a wider range of potential toxins. 5. Compared with parental species, hybrids of reciprocal hybridization had significant difference in feeding preference for hot pepper.

结果显示，广食性的棉铃虫在中肠羧酸酯酶和谷胱甘肽-S-转移酶活性方面高于寡食性的烟青虫，但烟青虫谷胱甘肽-S-转移酶活性可以被寄主植物内的烟碱和辣椒素所诱导，表明解毒酶活性在一定程度上影响着植食性昆虫的食性广度；同时也暗示着二近缘种对有毒的次生物质采取不同的适应策略：烟青虫有选择地对付种类较少的植物次生物质以减轻营养和能量的消耗；棉铃虫则必须对付存在于其寄主植物内的种类繁多的有毒次生物质。

In another experiment, cucumber leaves treated with pectinases extract and inoculated with Cladosporium cucumerinum were assayed for these defense-related substances.

以草酸青霉菌的固态发酵产物－果胶酶粗提液喷雾诱导黄瓜，研究了该激发子对黄瓜细胞内几丁质酶和β-1，3-葡聚糖酶2种病程相关蛋白以及细胞壁物质的影响。

However, the 3 groups from medium A exhibited the highest microbial diversity and best decolorization results with 99.53% and 97.42% color removal rate of Reactive Red M-3BE and Acid Red. From them, 16 strains of fungi were isolated and primarily identified as Saprolegniaceae, Eurotiaceae, Erysiphaceae and Physodermataceae . Fungi groups from medium B and D exhibited a bit lower color removal rate of various dyes and only 3 and 2 isolates primarily classified as Saccharomycetaceae and Eurotiaceae were obtained from them. Fungi cultures in medium A and B could produce lignin peroxidase, and those in medium D could be detected higher activity of laccase. All the fungal cultures exhibited very weak activity of manganese dependant peroxidase.

来自A培养基的3组菌群显示出最好的脱色效果和最大的菌群丰富度，对50mg/L的活性红M-3BE和酸性红A溶液的脱色率最高达到99.53%和97.42%，从中分离到了16株真菌，初步鉴定分属于水霉科、曲霉科、节壶菌科和白粉菌科；而B和D培养基中所获得的菌群脱色效果稍差，从中仅得到3株和2株真菌，初步鉴定属于酵母和青霉。A、B两种培养基在各种染料存在下更易产生木质素过氧化物酶，产漆酶能力较弱，而D培养基产漆酶活性较高。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

The influence of three kinds of heavy metal on the activities of peroxidase, superoxide dismutase,catalase and the contents of malondialdehyde, soluble protein and chlorophyll of Lemna minor was investigated.

对水体中主要的3种重金属对青萍抗氧化酶系统的几种酶，包括过氧化物酶、超氧化物歧化酶、过氧化氢酶的活性，丙二醛的含量，可溶性蛋白含量及叶绿体色素含量等指标的影响进行了研究。

The results showed:①Anthocyanin content in mature fruits was 132170 U·g-1FW in bud mutation which was very significantly higher than that in'Ralls'(49140 U·g-1FW);②Activities of PAL and UFGT were almost the same between the two materials, but CH I and DFR activitieswere significantly high in bud mutation, which were related to the increase of anthocyanin levels in the mutant;③Bagging treatment inhibited anthocyanin synthesis of bud mutation. But anthocyanin content had significantly increased after removing the bags. Activities of CHI and UFGT increased rap idly and were much higher than those in the control after the bag removal. The increase was consistentwith the imp rovement of anthocyanin levels.

结果显示：①在果实发育期间，芽变果皮的花青苷含量明显高于'国光'，尤其是成熟期芽变果皮花青苷含量为132170 U·g-1FW，而'国光'仅为49140 U·g-1FW；②在果实发育期间，两个品种间PAL 和UFGT的酶活性无明显差异，但芽变的CHI和DFR酶活性明显高于'国光'，表明芽变花青苷合成能力的提高与CHI和DFR酶活性高有关；③套袋抑制芽变果皮花青苷的合成，但解袋后花青苷的含量极显著升高，解袋后4种酶的变化趋势差异较大， CHI和UFGT活性均迅速升高，明显高于对照，这与解袋后花青苷迅速合成相吻合。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。