霉菌的

Material and method the 1.1 objects that monitor in January 2005 ~2007 year in December, nantong city disease accuses a center to monitor bar of hall of inn of the inn inside the area, communal bathroom, tonsorial hairdressing, song to wait public 771, every random and draw-out a certain number of the sample of public articles for use that the preparation after cleaning disinfection uses in all 4 960 portion. Crock of pool of the tea service of the 1.2 content that monitor and methodological inn, towel, bed sheet, face, bath, chamber pot undertakes group testing of bacterial gross, coliform measures; the towel of communal bathroom, bathrobe undertakes group testing of bacterial gross, coliform measure, slipper undertakes mould and microzyme detect the tea service of; public place of entertainment undertakes group testing of bacterial gross, coliform measures; the towel of tonsorial hairdressing place, scissors undertakes bacterial gross, coliform group detect with coccus of golden yellow grape.

梗概： 为了解南通市近几年公共场所卫生状况，客观评价公共场所卫生水平，为今后的卫生监督监测提供科学依据，对2005年~2007年南通市公共场所公共用品(茶杯、口杯、床上用品等)的细菌总数、大肠菌群等微生物检测进行了分析，结果报告如下。1材料和方法1.1监测对象2005年1月~2007年12月，南通市疾控中心监测区内的旅店、公共浴室、理发美容店、歌厅酒吧等公共场所771家，每家随机抽取若干件清洗消毒后预备使用的公共用品样品共4 960份。1.2监测内容和方法旅店的茶具、毛巾、被单、面池、浴缸、便桶进行细菌总数、大肠菌群检测；公共浴室的毛巾、浴衣进行细菌总数、大肠菌群检测，拖鞋进行霉菌和酵母菌检测；娱乐场所的茶具进行细菌总数、大肠菌群检测；理发美容场所的毛巾、剪刀进行细菌总数、大肠菌群和金黄色葡萄球菌检测。

The esterifying effects of monascus, daqu and rhizopus strain on single acid (acetic acid, caproic acid, lactic acid, butyric acid) and mixed acid (acetic acid,caproic acid,lactic acid, and butyric acid mixed together according to the ratio of 1∶1∶1∶1) were studied.

对红曲霉、大曲以及根霉菌对单一酸（乙酸、己酸、乳酸、丁酸）和混合酸（四大酸按1∶1∶1∶1混合）的酯化效果进行了研究，结果表明，红曲霉能促进己酸、丁酸及混合酸与乙醇的酯化作用，生成的酯类物质均为己酸乙酯，而且酯化能力极强；大曲能促进己酸、丁酸及混合酸与乙醇的酯化作用，生成的酯类物质均为己酸乙酯，并且能促进乙酸与乙醇的酯化生成乙酸乙酯；根霉能促进己酸和混合酸与乙醇的酯化作用，生成少量的己酸乙酯和乳酸乙酯。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Molds are aerobes, and are controlled by the absence of oxygen, Bacterial populations which will dominate a substrate may be manipulated by their oxygen requirements and its availability..

霉菌是需氧的，它受缺氧的控制。基质的控制。基质中占主导地位的各种细菌的菌数分布受各菌的需氧情况以及获得氧的难易的控制。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失