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The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind III. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536. The ligation mixture was transformed into EPI300 competent cells. A total of 5184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA,用Hind III部分酶解后回收40~60 kb大小的高分子量DNA,与质粒载体pIndigoBAC536连接,电击转化EPI300感受态细胞,经蓝白斑筛选共挑取了5184个白色克隆。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind Ⅲ. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536.The ligation mixture was transformed into EPI300 competent cells. A total of 5 184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA,用HindIII部分酶解后回收40kb~60kb大小的高分子量DNA,与质粒载体pIndigoBAC536连接,电击转化EPI300感受态细胞,经蓝白斑筛选共挑取了5184个白色克隆。

Vmutageniz, We gained a stain of Aspergillus niger S13 with high cellulase production, Further more we research on the optimum solid culture a wheat stem 70 % bran 30 %, nitrogen source is yeast extract 0.68%,(NH4)2SO4 0.35%, trace element 0.1%, Vogel's solution 4 %, pH3.5, temperature 30℃, cultivation time 72-96hr, With such condition, its β-glucosidas activity reached 801.18mg·glucose/g·h, CMCase activity 6262.46mg·glucose/g·h filter paper activity 20.73IU.

本文通过分离筛选和紫外诱变法,选充出一株高产纤维素酶黑曲霉菌株S13,并进行了最佳固态发酵培养条件的研究,其结果为:最佳碳源为1%碱洗麦杆粉70%,麸皮30%;另加酵母膏0.68%,(NH_4)_2SO_4 0 35%,微量元素0.1%,Vogel's母液4%,初始pH3.5培养温度30℃,培养时间为72~96 小时,在此条件下其β-葡萄糖苷酶活力达801.18mgG/h·g,CMC酶活6262.32mgG/h·g,滤纸酶活20.73IU。

The effects of pyridine substitutive derivations on the metabolic pathway of Blakeslea Trispora, were studied such as 2,6-dimethylpyridine,2,5-dimethylpyridine, 2-amino-6-methylpyridine and tobacco dust which contains mainly 3-pyridine substitutive derivation.

主要考察了吡啶衍生物如2,6-二甲基吡啶、2,5-二甲基吡啶、2-氨基-6-甲基吡啶及烟草废弃物(主要成分为3-吡啶基衍生物)对三孢布拉氏霉菌代谢途径的影响。

On soil fungi, Fusarium root shell mold, Rhizoctonia, Pythium, Miao-rot fungus, Corticiumpathogens have a significant effect on the damping-off,diseases, damping-off, Fusarium wilt, Verticillium, Sclerotinia, Colletotrichum, disease, dry rot, scab, Sclerotinia rot, seedling blight, stem blight, leaf blight, steep root, even the special effects for the continuous cropping obstacles.

对土壤真菌、镰刀菌、根壳菌、丝核菌、腐霉菌、苗腐菌、伏革菌等病原菌都有显著的防治效对立枯病、烂秧病、猝倒病、枯萎病、黄萎病、菌核病、炭疽病、疫病、干腐病、黑星病、菌核软腐病、苗枯病、茎枯病、叶枯病、沤根、连作重茬障碍有特效。

The isolate Paecilomyces farinosus sw03032 was cultured in a same liquid medium at 25℃ and the impact of rotate speed of rocking bed,initial inoculum and initial pH value on mycelial bioass were studied.

在25℃下同一种培养液中,研究了摇床转速、接种量和初始pH值对粉拟青霉菌丝生长量的影响。

Under normal circumstances when plents die,they are exposed to air and are broken down primarily by oxidation and also by various organisms,particularly the fungi and aerobic bacteria.

正常情况下,植物死亡后会暴露于空气中。它们主要会被氧化分解,也会被不同的微生物分解,尤其是,霉菌和好养细菌。

Aspergillus species have emerged as an important cause of life-threatening infections in immunocompromised patients.

在免疫受损患者中,曲霉菌感染已经成为威胁生命的重要感染因素。

Results show that mercuric chloride is an effective chemicals. Optimum treatment by mercuric chloride is 0.128%concentration and 461 second on bacteria-bearing wheat seed.

结果表明:没处理的种子由于发芽时100%长有霉菌,正常幼苗率只有40%,而此批种子经生活力测定结果为生活力64%。

Mildew killed the rosebuds in our garden.

霉菌把我们园中的玫瑰花蕾弄死了。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。