霉菌病的

The pathogen causing lily blights was isolated from diseased lily with typical symptoms on basal stem. The pathogen was identified as Phytophthora nicotianae van Brede de Haan according to the characteristics of mycelium, chlamydospore, zoosporangium, oospore and the test of pathogenicity of the pathogen.

从具典型症状的新鲜百合疫病植株茎基部病组织中分离到百合疫霉菌，根据其病原菌菌丝的形态、菌落特征，厚垣孢子、游动孢子囊和卵孢子的形态和大小，以及病原菌致病性测定，该病原菌鉴定为烟草疫霉 Phytophthora nicotianae van Brede de Haan。

Since more and more soybean was imported into China, to establish rapid and accurate molecular detection methods to Phytophthora sojae are very important to soybean trade between China and other countries,also is necessary to control the propagation of Phytophthora root rot.

因此，建立大豆疫霉菌快速准确的分子检测方法，不仅对我国与其他国家的大豆贸易至关重要，而且对控制大豆疫霉病在我国的传播也是必不可少的。

Among the patients, fungal infections were correlated with male gender and bedridden status while xerosis was associated with increasing age. For scabies infection, a previous history of scabies was the most significant statistical risk factor.

针对病患来看，统计上发现霉菌感染与男性性别及长期卧床相关，乾皮症与年龄相关，而疥疮感染的危险因子主要为过去的疥疮病史。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Most other Sclerotinia or white mold diseases, such as on dry beans, soybean, canola and sunflower head rot are initiated by and infection of above ground plants parts by ascospores.

大多数其他的核盘菌或白霉菌，干豆、大豆、向日葵等作物的热腐病都是由于子蘘孢子浸染植株的地上部分所致。

Two outstanding fusants SR-7 and SR-T19 were chose out in the fusion group 90-11×FR-008, the former produces not only antibiotics the parents produced but also a new antibiotic effective gainst a wide range of both gram positive and negative bacteria, which the parents could not produce, and its product of the 37℃ fermentation was very effective against the cotton seedling damping off caused by Rhizoctonia sonali while product of the 26℃ was effective agaist the bacterial rots of turnip and Chinese cabbage; the latter have a much stronger activity against a wide range of fungi and yeasts than the parents.

在90-11×FR-008融合组合中，筛选获得2个优良融合子SR-7和SR-T19，前者除产生亲本产生的抗生素外，还产生亲本不产生的一种新的具有广谱抗细菌活性的碱性水溶性抗生素，防病试验表明，其37℃发酵产物对棉花苗期立枯病具有良好的防治效果，28℃发酵产物对萝卜和大白菜具有明显的防腐作用；后者与亲本FR-008的抗菌谱完全一致，但其活性强度都超过了FR-008；采用RAPD分子标记技术检测了SR-7和SR-T19与亲本的同源性及彼此间的差异，从而首次直接从DNA分子水平上验证了链霉菌的原生质体融合子及其遗传重组的多样性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失