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A single donor cell was injected into the perivitelline space of enucleated oocyte and fusion was induced with electric pulses. Reconstructed oocytes were activated using ionomycin (5 M,5min) and 6-dimethylaminopurine (6-DMAP,2mM,5h).

将供核细胞注射到去核卵母细胞的卵周隙,经电融合获得重构胚,再经离子霉素(Ionomycin,5μM,5min)和6-二甲基氨基嘌呤(6-DMAP,2mM,5h)处理激活。

The Peronosporales contains saprobes and many harmful plant parasites.

霜霉目包括腐生菌以及许多有害的植物寄生物。

After assaying the sequence of 28S and sequence alignment,it was found that the similarity of four strain of P.herbarum with that of high pathogenic strain was over 98%,thus confirming from molecular level that strain belonged to Phoma herbarum.

通过测定菌株28SrDNA序列并进行序列比对,有4个草茎点霉菌序列与01菌株的相似性达98%以上,从分子水平进一步证实01菌株为草茎点霉。

Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV spectrophotometry and RP HPLC.

提取黄瓜炭疽病菌 Colletorichumlagnarinm 、青菜炭疽病菌 Phomafiltrate 、棉花枯萎病菌 Fusariumoxysponum 、黑曲霉 Asperillusniger 中的真菌诱导子与人参毛状根共培养,应用HPLC及比色法对培养物中总苷及几种单体皂苷进行分析测定。

Apoptosis induced by boanmycin in human nasopharyngeal cancer SUNE-1 cells was tested by fluorescent microscope, flowcytometry and DNA gel electro phoresis.

应用荧光显微镜、流式细胞仪及琼脂糖凝胶电泳等方法检测博安霉素诱导人鼻咽癌SUNE-1细胞凋亡的作用。

Cis-1-Propenyl phosphonic acid is the key intermediate in the synthesis of phosphonomycin, a new broad antibacterial drug.

顺丙烯膦酸是新型广谱抗菌药磷霉素合成的关键中间体。

A total of 1971 strains of endophytic fungi was isolated. As a result of morphological identification 32 genera were obtained, of which Alternaria (amounting to 18.01% of the total), Phyllosticta (14.66%) and Rhizoctonia (14.56%) were dominant.

结果表明:所分离出的1971株内生真菌可归于32属,以交链孢属Alternaria(18.01%)、叶点霉属Phyllosticta(14.66%)和丝核菌属Rhizoctonia(14.56%)为优势菌群。

The antitumor and antifungal activities of BFA on four human tumor cell lines and six phytopathogenic fungi were tested,and the minimum inhibitory concentrations on sensitive fungi were tested too.

进一步研究表明,BFA对人Burkitt's 淋巴瘤细胞株Raji、人肝癌细胞株HepG2和人非小细胞肺腺癌细胞株A549具有较强的抑制活性,对植物病原真菌黑曲霉和杨桃炭疽菌等具有抑菌活性。

Late blight, caused by Phytophthora infestans, is most devastating disease of crops worldwide, in particular for potato.

由致病疫霉Phytophthora infestans引起的晚疫病是全球第一大作物病害,对马铃薯生产危害非常严重。

The effect of activities of polygalacturoase on pathogenicity in Phytophthora sojae was investigated.

就大豆疫霉根腐病菌多聚半乳糖醛酸酶(Polygalacturonase,PG)活性对其致病力的影响进行了研究。

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They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性,但我大喊,并在他们的方向扔石头让他们过的线索,远离我,以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。

Store this product in a sealed, lightproof, dry and cool place.

密封,遮光,置阴凉干燥处。