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They are associated with an impressive spectrum of diseases in humans, ranging from benign colonization of the lung tQ life-threatening diseases such as invasive aspergillosis or allergic bronchopulmonary aspergillosis.

它们与人类的许多疾病有关,可以从普通的过敏反应到肺的移行性病及一些致命的变态反应性疾病,如侵袭性的曲霉病或过敏性支气管肺曲霉病。

The research on the conditions of chlamydospore production for the liquid fermentation showed that, the better single factors were oat liquid media, 30℃, 120r/min surge culture, sucrose as carbon source,(NH_4)_2SO_4 as nitrogen source and the ratio of 20 to 1 for C/N. Under the better single factors mentioned above, the largest quantity of chlamydospore, i.e.3.05×10~7spores/ml, was obtained on the 10th day after culture.

木霉T-33菌株产生厚垣孢子的发酵条件研究表明,采用燕麦粉培养液、30℃、120r/min振荡培养、250ml三角瓶装入80ml培养液、接种5片活化2d的5mm直径的菌片,碳氮源选择蔗糖和硫酸铵,C/N为20/l,足适合T-33菌株产孢的最佳单因素条件(碳氮源实验基于Czapek培养基);用这些最佳单因素组合(不包括碳氮源和C/N结果)培养木霉T-33绘制了产孢曲线,发现在培养第10d得到最大的厚垣孢子产量3.05×10~7个/ml。

How to ruduce the content of citrinin is a problem need to be solved urgently.

红曲的药用价值和保健功能日益引人关注,然而红曲中桔霉素的存在严重制约着红曲产品的开发,如何降低红曲桔霉素的含量是一个迫切需要解决的问题。

Sequence analysis and function of the genes were carried out.

一方面,利用已知的尼可霉素生物合成相关基因的DNA片段为探针,从圈卷产色链霉菌的cosmid文库中克隆出6个与尼可霉素生物合成相关的基因,并进行了基因的序列分析和功能研究。

In recent years, retrograde adriamycin sensory ganglionectomy has been used in treatment of trigeminal neuralgia and has obtained good therapeutic effect The mechanism of action, particularly, of the toxic effects with different mechanism methods and choice of doses for muscle cells and nerve cells is still unclear.

阿霉素为常用的抗肿瘤药,近年来应用阿霉素治疗三叉神经痛的文献报道较多,且已获得较好的效果,但其治疗机制尚不十分明确,尤其是对肌细胞及神经细胞的毒性作用机制特点及不同的给药方式和剂量选择尚不够明确。

In order to find out the proteinase suitable for soybeen peptide preparation,studies were conducted on the properties of mucor producing protease,the preparation method of mucor protease,the decomposition of soybeen protein isolated by mucor protease,and the molecular weight of the enzymatic decomposition products.

为探寻适合于制备大豆多肽的蛋白酶,对毛霉产蛋白酶的特性、毛霉蛋白酶的制备方法及其对大豆分离蛋白的降解进行了研究,并测定了酶解产物的相对分子质量。

The properties of Mucor protease are studied. The Mucor protease are multienzymes that contain neutral and alkaline protease that have optimum pH in 9. 4 and a stable pH range 5. 5~10.The optimum temperature for neutral is in 60 ℃ and alkaline in 50℃. The activity of enzymes is stable for 100min in 40℃ and 50℃ incubation.

二、检测了毛霉蛋白酶的性质,结果表明毛霉蛋白酶系以中性及碱性蛋白酶为主,该酶系的最适pH为9.4,在PH5.5~10的范围内稳定,中性蛋白酶的最适温度为60℃,碱性蛋白酶的最适温度为50℃,在40℃、50℃保温100min酶活稳定,属于中高温型蛋白酶。

But the hydrolysate has strong bitter taste and has to get rid of during the following process. Mucor has been used in sufu, a well-known delicious Chinese cheese that is made with soybean curd, making in China for hundreds years.

腐乳毛霉能够产生一定数量的蛋白酶,可以将豆腐坯中的大豆球蛋白(11S)水解为分子量较小的肽类(P.C.L0)并具有良好的风味,毛霉蛋白酶有可能成为制备大豆多肽的优选酶种。

breast cancer cell line MCF-7 were marked killed by trametes robiniphila murr in certain concentration. The IC50 of trametes robiniphila murr in three days to MCF-7 was about 0.05mg/ml. Its cell toxin were increased while the touched time was delayed or the concentration was advanced. Trametes robiniphila murr also can advance cell toxin of adriamycin and reverse resistance of MCF-7/Adr.

用MTT 法测定不同浓度的槐耳浸膏作用不同时间下对乳腺癌细胞系MCF-7 的杀伤作用,同时测定应用槐耳浸膏时对阿霉素的增敏作用和耐药逆转作用;结论:金克在一定范围内对乳腺癌细胞系具有明显的杀伤作用,其作用3 天时,IC50 在0.05mg/ml 左右,随着药物浓度升高和作用时间的延长其杀伤作用相应的增强,对阿霉素化疗具有增敏作用和逆转耐药作用。

Experiment of the third part use the first part"s production as carrier, selecting Geotrich candidum and Candida tropicalis of higher production of protein also selecting producing the cellulose enzyme Bacillus subtilis FS and Lactobacillus bulgaricus by ourselves separated, design orthogonal test and a series of single factor experiment, finally assurancing the inoculation quantity of four strains germ, is Geotrich candidum 5%, Candida tropicalis 5%,Bacillus subtilis FS 5% of FS, Lactobacillus bulgaricus 3%, the most suitable development temperature is 30 "C , the development time is 15 days.

实验的第三部分是以第一步共培养所得的培养物为载体,选择产蛋白量高的白地霉、热带假丝酵母及本室筛选的产纤维素酶芽孢杆菌FS和自行分离的保加利亚乳酸杆菌,设计正交实验和一系列单因素实验,最终确定了四株菌的接种量,分别为白地霉5%,热带假丝酵母5%,FS5%,保加利亚乳杆菌3%,最适培养温度为30℃,培养时间为15天。

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