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In this paper, the garlic oil was prepared by extraction with aether as solvent.

采用乙醚萃取法提取了大蒜油,并研究了大蒜油对木霉和根霉的抑菌作用。

Inhibition effect of 25 kinds of preservatives to botrytis cinerea and alternaria spp from red global grapes were studied.

研究了25种防腐保鲜剂对红提葡萄中灰葡萄孢霉和交链孢霉的抑制效果。

In this study ten species of Trichoderma were morphologically identified. Among them, Trochoderma koningii was systematically studied based on its physiological and biochemical characteristics. Its antagonistic mechanism and biocontrol were studied. In addition, optimizing condition of antibiotics protruded and fermentation on culturing materials were also tested. Results as following:1. For the conidium germination of T.

本研究对所分离到的十种木霉进行了形态学分类鉴定,在此基础上对其中的康氏木霉的生理生化特性进行了较为系统的研究,同时对康氏木霉的拮抗机制、产毒条件优化、固体培养条件优化、生物防治等进行了研究,其结果如下:一,测定了康氏木霉菌落生长、孢子萌发与温度、相对湿度、pH值、营养物质、光照等的关系。

For example, the growth of N-fixating bacterium and Mucor take base to the environment while Rhizopus take acid to it.

如固氮菌和毛霉的生长易使环境转向碱性,根霉使环境变酸。

The results showed that mucor protease was a kind of enzyme of strong induction. In addition to temperature,moisture and culture time,the ability of mucors producing profeinase were also closely related to culture methods,for example,the enzyme activity increased greatly when mucor was cultured with wheat bran and the leaven was turned over once as a lot of spores appeared.

结果表明,毛霉蛋白酶是诱导性很强的酶类,毛霉的产酶条件除与温度、培养时间、相对湿度等有关外,还与培养方式密切相关,以麦麸培养毛霉,当长有大量孢子时翻曲1次,可大幅度提高酶活力。

The antimicrobial test and researched was done on wide distillation of different solvents on Terpenoid, polarity separation of ethanol distillable substance, thin layer chromatography of different polarities and distillation of amylase. The results showed that the active substance distilled by 95% ethanol and V methanol: toluene=3:1 had stronger antimicrobial activity on Agaricus blazei Murrill. The antimicrobial activity of ethyl acetate was stronger than wide distilling substance with ethanol, but the water part has not antimicrobial effect. Two straps of ethyl acetate phase(Rf0.57、Rf0.63)(0.1g/ml)had stronger antimicrobial activity on Agaricus blazei Murrill, the antimicrobial zone on Pseudomonas was 19.3mm. The bacteriostatic activity of chromatography strap of water phase was rather weaker, the water solution (0.5g/g) of amylase had better effect on pathogenic fungus of Agaricus blazei Murrill, its antimicrobial zone on Aspergillus niger, Citrinin, Trichoderma viride were 10.8 mm, 7.9 mm, 5.3 mm.

通过对日本凹顶藻以不同溶剂的粗提、乙醇提取物的极性分离、不同极性的薄层层析和多糖的提取,进行抗菌试验的研究,发现95%乙醇和V甲醇:甲苯为3∶1的提取的活性物质具有较强的抗姬松茸的病原细菌活性,乙酸乙酯相对病原细菌的抑菌强于乙醇的粗提物,而水相基本无抑菌效果,乙酸乙酯相的两个带(Rf0.57、Rf0.63)浓度为0.1g/ml具有较强抗姬松茸病原细菌的活性,对假单孢杆菌抑菌圈达到19.3mm,水相层析带的抑菌活性较弱,多糖的粗提物配成0.25g/g的水溶液对姬松茸病原真菌有较好的抑菌效果对黑曲霉、桔青霉和绿色木霉的抑菌圈分别达到10.8 mm、7.9 mm、5.3 mm。

Methods According to differences in internal transcribed space sequences of Penicillium genus and specific IAO sequence, we designed species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum and established the corresponding PCR systems.

根据 GeneBank 中青霉属不同种和相近属种的 ITS 序列差异和灰黄青霉特有的 IAO 序列,设计了污染区优势种灰黄青霉的特异性引物 AS1/RS4 和 IAO1/IAO2,建立相应的特异探针检测体系。

The CDA gene shares high sequence similarity with that of fungi including Rhizopus oryzae (75%), CDA1(58%) and CDA2(56%) of Rhizopus circinans, Mucor rouxii(56%), Gongreonella bulteri(48%), Rhizopus stolonifer(39%), Phycomyces blakesleeanus(39%), CDA1(17%) and CDA2(16%) of Saccharomyces cerevisiae.

总状毛霉CDA基因与其它相近种米根霉、卷柄根霉的CDA1和CDA2、鲁氏毛霉、卵形孢球托霉、匍枝根霉、布拉克须霉、酿酒酵母的CDA1和CDA2的基因序列同源性分别为:75%、58%、56%、56%、48%、39%、39%、17%和16%;相应的氨基酸序列的同源性分别为:69.0%、57%、59%、55%、47%、30%、32%、18%、21%。

Result The phytopathogenic fungus Pythium aphanidermatum was isolated; 7 strains of antagonistic actinomycete, which had obvious inhibitory effects on Pythium aphanidermatum were screened. The inhibitory ring of No.8 strain was 13.3 mm, and reached 88.3% when cultivated for 120 h.

伽师甜瓜猝倒病的病原菌为瓜果腐霉;筛选出7株对瓜果腐霉具有显著拮抗性的生防放线菌,其中8号放线菌对瓜果腐霉的拮抗环宽度为13.3mm,其无菌发酵滤液对瓜果腐霉的抑菌率在培养120h时高达88.3%。

Boehmeriae were studied, and the results indicated that the zoospores of P. boehmeriae could be inactivated obviously by irradiating with 30W ultraviolet light 50cm away from the culture dishes tested and that 95% germination of zoospores could be restrained by 30W ultraviolet light treatment for 40sec.The mycelium growth from mycelium discs of P. boehmeriae were inhibited mostly by 30W ultraviolet light treatment for 20min. Afterwards, the inhibitive rates were decreased with the rise of the treatment time, which showed that the mycelium discs of P. boehmeriae had produced flexibility to ultraviolet rays after being irradiated by certain dosage of ultraviolet light.

研究了紫外光对苎麻疫霉生物学特性的影响,结果表明,30W的紫外灯管距培养皿50cm照射处理对苎麻疫霉的游动孢子具有明显杀灭作用,紫外光照射40sec可以抑制95%的游动孢子萌发;紫外光照射20min对菌丝块的菌丝生长的抑制率达最大,以后随着处理时间增加,抑制率却有降低的趋势,说明苎麻疫霉的菌丝块,经一定剂量紫外光照射后对紫外线产生了适应性。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

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但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。