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After heating at 80℃ for 60 minutes, the inhibition effect of vanillin against bacteria and Aspergillus was not be affected.

香兰素经80℃、60 min处理后不影响其对细菌、黑根等的抑制效果。

Expression of mutant tumor suppressor gene p53 in egg-type chicken with avian leukosis subgroup J was detected to study cancericidally molecular mechanism of ALV-J. Using immunohistochemistry method of strep avidin-biotin complex, many organs of illed egg-type chickens, involved liver, kidney, ventriculus glandularis, tumor, heart, pancreas, bone marrow, spleen, oviduct, lung, duodenum, thymus and bursa of fabricius were studied.

为了探讨J亚群禽白血病病毒的致癌分子机理,观察蛋用型鸡发生J亚群禽白血病时p53抑癌基因在各脏器中的表达,应用链亲和素-生物素-过氧化物酶复合物免疫组化法,研究了蛋用型鸡J亚群禽白血病自然病例的肝脏、肾脏、腺胃、肿瘤、输卵管、心脏、胰脏、骨髓、脾脏、肺脏、十二指肠、胸腺和法氏囊。

The Abbot of La Trappe was by right the vicar-general of the congregation as soon as his election was confirmed by the president-general of the Order of Cteaux.

该住持的香格里拉大是由权利的副主教,一般的聚集尽快当选证实了布什总统提出的命令

I'm taking a moment just imaginin' that I'm dancin' with you I'm your pole and all you're wearing is your shoes You got soul, you know what to do to turn me on until I write a song about you And you have your own engaging style And you've got the knack to vivify And you make my slacks a little tight, you may unfasten them if you like That's if you crash and spend the night But you don't fold, you don't fade You've got everything you need, especially me Sister you've got it all You make the call to make my day In your message say my name Your talk is all the talk, sister you've got it all Curl your upper lip up and let me look around Ride your tongue along your bottom lip and bite down And bend your back and ask those hips if I can touch Because they're the perfect jumping off point of getting closer to your Butterfly Well you float on by Oh kiss me with your eyelashes tonight Or Eskimo your nose real close to mine And let's mood the lights and finally make it right But you don't fold, you don't fade, you've got everything you need Especially me Sister you've got it all You make the call to make my day In you message say my name Your talk is all the talk sister you've got it all You've got it all, you've got it all, you've got it all (2x) Doll I need to see you pull your knee socks up Let me feel you up side, down slide, in slide, out slide, over here Climb in my mouth now child Butterfly, well you landed on my mind Dammit you landed on my ear and then you crawled inside Now I see you perfectly behind closed eyes I wanna fly with you and I don't wanna lie to you Cause I, cause I can't recall a better days I'm coming to shine on the occasion You're an open minded lady You've got it all And I never forget a face If I'm making my own I have my days Let's face the fact here, it's you that's got it all You know that fortune favors the brave Well let me get paid while I make you breakfast The rest is up to you, you make the call You make the call to make my day In your message say my name Your talk is all the talk, sister you've got it all Cause I can't recall a better day I'm coming to shine on the occasion You're a sophisticated lady, oh you've got it all You've got it all, you've got it all, you've got it all (3x) You've got it all, you've got it all….

我是在一窝窝成像一刻',我是丹素'你我是你的杆位和所有你穿你的鞋你有灵魂,你知道该怎么做,我就打开直到我写一首歌曲和你有自己的风格进行和你一把刷子为维维风云你打我的裤子有点紧,你可以解开他们,如果你喜欢这是如果你发生碰撞过夜但是,你不能折叠,你不褪色你有你需要的一切,尤其是我妹妹你有这一切您拨打电话让我的一天在您的邮件说我的名字你说是大家都在谈论,姐姐你有这一切你的上唇卷曲起来,让我环顾四周凑凑热闹你的下嘴唇咬自己的舌头,下和弯曲你的背部和臀部的要求,如果我可以触摸因为他们的完美跳的越来越近点的轻便铁路蝴蝶站那么你就浮动噢亲吻你今晚我的睫毛你的鼻子或爱斯基摩人真正接近我让心情的灯光,终于使正确但是,你不能折叠,你不褪色,你有你需要的一切尤其是我妹妹你有这一切您拨打电话让我的一天在你的信息说我的名字你说是大家都在谈论你妹妹了这一切你有这一切,你有这一切,你有这一切(2倍)娃娃我要看到你拉你的膝盖袜子了让我觉得你边上,向下滑动,在幻灯片,幻灯片出在这里,在我的嘴里攀登现在的孩子蝴蝶,以及你降落在我的脑海就叫你落在我的耳朵,然后里面爬现在我看到完全不公开你的眼睛我想和你飞,我不想骗你原因一,原因我不记得一个更好的日子我来照留念你是一个开放的态度夫人你有这一切我永远不会忘记的面孔如果我做我自己我有我的天让我们面对事实,在这里,是你的了一切你知道财富的勇敢的主张那么让我得到报酬,而我让你早餐接下来就看你,你拨打电话您拨打电话让我的一天在您的邮件说我的名字你说是大家都在谈论,姐姐你有这一切原因我不记得一个更好的一天我来照留念你是一个复杂的夫人,哦,你有这一切你有这一切,你有这一切,你有这一切(3倍)你有这一切,你有这一切。。。。

Goodyear spent another four years,in which things went very badly with him,before he made the discovery how to vulcanize rubber completely.

固特异又化了四年的时间,在这四年中,运一直跟着它,直到发现如何使橡胶完全硫化的方法。

It was not wet yet cold and dark and flooded a road This road is digesting the old one You can hear the sound of gravel, cement and water wagon tracks which the wind wasn't quick enough to smooth away a corpse frozen by snow, and a car leaving with happy songs The most faithful year, or two years its biggest sore is that it's not been hurt The molds on the road are home for magpies In the distance, the sea overflows by itself like people who live outside life vaguely derailing Time will make them very poor and prove that great pique can be disaster to them, though that's not happened yet I've walked along this road, it was once occupied by grass and dirty snow Where there's now a lamp post, I used to loiter.

它不是湿的却是冷而黑的将一条道路淹没这条道路,正在消化着原来的道路你会听到一些沙石的声音,一些水泥和水风来不及吹走的车辙一块积雪冻僵的尸体,还有一辆汽车满载快乐而去的歌声最忠诚的一年,或两年它最大的伤害是没有受到伤害道路上的斑,是喜鹊的家园远处的海水空自泛滥那只是生活以外之人一次不着边际的出轨时间会让它们一贫如洗并证明巨大的怨气于他们是灾难,但没有发出来我走过的这条道路,曾被青草或肮脏的雪们占领落脚之地如今站立着灯柱。

Patterns of purification of peroxidase having lignin or manganese oxidation activities in white rot fungi have been extensively studied.

关于青产生木素过氧化物酶的研究在国外只有零星报道,但尚未有关于其纯化及特性的研究,国内也无此方面的报道。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X,脂质体转染HEPG2细胞;潮素选择培养稳定表达X基因的HEPG2-X细胞;免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Result The zoospore progenies of isolate HN8-Mt and isolate HN3-Mt had the similar characteristics such as growth rate, colony morphology and the resistance to metalaxyl with that of their parents.

结果]辣椒疫Mt突变株的生长速率、菌落形态以及对甲霜灵的抗性等性状在其游动孢子后代中未发生变异。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。