需要的

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

My father always said: a child needs four things----enough love,nutritious food, regular sleep, plenty soap and water---besides these, he needs most is some sensible indulgency.

我父亲总是说，一个孩子需要四样东西--充分的爱、富于营养的食物、有规律的睡眠、大量的肥皂和水--这些完了呢，他最需要的是一些明智的放任。

To confirm your reservation with the ticket 1, the day of booking and the day of the vote送票: In the civil aviation system after successful booking, customer service center will provide you with the confirmation in accordance with the manner with you to confirm the booking of flights and prices, and you need送票time for you送票; the event booking is unsuccessful, call center phone will notify you as soon as possible, so please be sure to keep you filled in Tel flow 2, the first book, not counting: If you would like to book machine bit later on the ticket, please indicate your deadline for the final out of votes (if you requested time limit for the final out of votes and the provisions of airline tickets contravene the time limits, the call center will be promptly notified of your final votes in the time limit), if not to specify you may be required to bear the loss Refund; Because the airlines have the right to cancel any reservations unbilled bit machine, so when you out in the final period of time decided to vote the ticket, please immediately notify the call center phone, we will call the day of your again for you to confirm your reservation slots previously the case.

确认预订与出票 1、当天预订并当天出票送票：在民航系统中订位成功后，客服中心将按照您提供的确认方式与您确认所预订的航班以及价格，并按您所需要的送票时间为您送票；万一订位不成功，客服中心将会尽快电话联系通知您，故请务必保持您所填的联系电话畅通 2、先预订，不出票：如果您希望先预订机位稍后出票，请注明您最后出票期限（如果您要求的最后出票时限与航空公司之规定出票时限抵触时，客服中心会及时通知您最后出票时限），如果不注明您有可能需要承担退票损失；由于航空公司有权取消任何预订未出票的机位，因此当您在最后出票时限内决定出票后，请立即电话通知客服中心，我们会在您来电当天再为您确认一下您先前预订的机位情况。

Have Flex Vml Svg Ajax Applet Activex commonly usedly at present I divide these technologies into two kinds of Flex Vml Svg Ajax for the moment is to use script technology, pure do develop to attack for the graph, do not have a problem absolutely, and those who do is more beautiful, but most circumstance falls, the develop that we need attacks the graph needs to have menu, other interface, user alternant, data processing, this kind is based on script technology, handling this side is too convenient really, be being developed especially and debug on, what come as programming language is convenient. Java Applet and Activex are to attribute programming language skill, without doubt, have powerful interface and process designing capacity, and can ensure whole group is same technology language, can develop in compositive development environment, debug.

目前常用的有flex vml svg ajax applet activex我姑且把这些技术分成两类flex vml svg ajax都是采用脚本技术，单纯做拓扑图来说，绝对没有问题，而且做的更漂亮，可是多数情况下，我们需要的拓扑图需要有菜单、其他界面、用户交互、数据处理，这种基于脚本技术的，在处理这方面实在是太方便了，特别是在开发和调试上，不如编程语言来的方便。java applet 和activex是属于编程语言技术，毫无疑问，具备强大的界面和编程能力，而且可以确保整个团队都是同一技术语言，可以在集成开发环境中开发、调试。

Then,the author analyzes the motive of domestic legislation, and adduces a foreign expert's statement :the basic motive of China importing the IP law comes from the policy of reforming and opening, i.e China needs international trading, international investment, and gets the urgent technology and equipment from the west country.

随后，文章对国内立法的动机作了分析，认为我国的集成电路立法可以用一国外专家的话来说：&中国引进知识产权法的根本动机是来自对外开放政策的驱使，中国需要对外贸易，吸引外资以及从西方获取迫切需要的技术和设备&，因此就必然承受发达国家所设定的限制和压力。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

A reasonable capital plans, should be to be below the premise that makes sure normal production manages need, dig latent capacity of enterprise interior capital hard, active organization is devoted, managing defray, the integrated balance of link of the balance that carries fund income and expenses, balance of supply and demand of goods and materials, sale, it is a foundation in order to run a plan, with the branch the plan has an authorized strength for the basis, strengthen the plan management of capital, analyse capital trends, according to the plan, need of check and ratify, can make the use of capital achieves optimization.

一个合理的资金计划，应是在保证正常生产经营需要的前提下，努力挖掘企业内部资金潜力，积极组织投入，节约支出，通过资金收支的平衡、物资供需的平衡、营销环节的综合平衡，以经营计划为基础，以部门计划为依据进行编制，加强资金的计划管理，分析资金动态，根据计划，核定需要，可使资金的使用达到最优化。

In order to maintain the blade of the sword cutter, extended service life, once the best cut-paper no more than 40 mm thick, and cutter on paper pressure should also be appropriate, and generally soft paper need pressure larger, harder materials require relatively little pressure.

为了保持刀片的锋利，延长切纸机的使用寿命，一次裁切的纸张最好不要超过40mm厚，而且切纸机对纸张的压力也应适当，一般较软的纸需要的压力大一些，较硬的材料需要的压力相对要小一些。

In the cutter in use and in order to maintain the blade of the sword cutter, extended service life, once the best cut-paper thickness should not exceed 40 mm, and cutter on paper pressure should also be appropriate, and generally soft paper need pressure larger, harder materials require relatively little pressure.

在切纸机使用中，为了保持刀片的锋利，延长切纸机的使用寿命，一次裁切的纸张厚度最好不要超过40mm，而且切纸机对纸张的压力也应适当，一般较软的纸需要的压力大一些，较硬的材料需要的压力相对要小一些。

However, to construct a tensor product orthonomal wavelet basis in L〓, 2〓-1 different functions are used. Furthermore, it is concluded that the family, obtained by dilations and translations from this radial wavelet as well as their linear combinations, can constitute an orthonomal basis in L〓. The conclusion is a major breakthrough in multidimensional wavelet analysis.

从而得到：由一个径向小波的伸缩、平移系及其线性组合可以构成n维平方可积函数空间L〓的规范正交基，这个结果将当前利用张量积方法构造n维正交小波基所需要的2〓-1个不同的函数降为仅需要一个径向小波函数，这在理论上是一个重大突破；构造了同时具备局部支撑和无穷次连续可微性质的高维不可分小波的例子，这是不同于I。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。