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ABSTRACT 1. Effects of cycloheximide and cytochalasin B on the parthenogenetic development of buffalo oocytes were investigated.

系统探讨了放线菌酮和细胞松弛素B对水牛卵母细胞孤雌激活的影响。

Methods:(1) The two-sided ovary of female animal and the two-sided testicle of male animal were excised. After the XLGBC to treat 28 days, the estradiol and the teststerone of serum were checked;(2) With intramuscular injection the Hydrocortisone,the Yangxu animals was made. After the XLGBC to treat 10 days, the testicle coefficient,the spermathecal gland coefficient and the ovary coefficient were observed;(3) With ig the Thyroid tablets and the Reserpine, the Yinxu animal was made. After the XLGBC to treat 10 days, the testicle coefficient,the spermathecal gland coefficient and the ovary coefficient were observed.

(1)雌性动物摘除双侧卵巢,雄性动物摘除双侧睾丸,复制成去势动物模型后予仙灵骨葆胶囊治疗28天,检测血清雌二醇和血清睾酮;(2)肌注氢化可的松,复制成阳虚动物模型后予仙灵骨葆胶囊治疗10天,观测并计算动物的睾丸系数、精囊腺系数和卵巢系数;(3)灌胃甲状腺片和利血平,复制成阴虚动物模型后予仙灵骨葆胶囊治疗10天,观测并计算动物的睾丸系数、精囊腺系数和卵巢系数。

Methods: Both 58 simple obese male adolescents and 46 normal-weight controls were subjected to exam the natural lengths of penis, mean volume of testes, the first time of spermatorrhea, pubic hair stage. The levels of luteinizint hormone, follicle-stimulating hormone, total testosterone, estradiol (E 2), PRL and fasting insulin concentrations in serum were detected by RIA.

测量5 8例单纯肥胖青少年和4 6例同年龄、同身高体重正常者的体重指数、臀围比值、阴毛发育程度、首次遗精发生率、阴茎大小、睾丸体积,放免法测定血清黄体生成素、卵泡刺激素、总睾酮、雌二醇(E2 )、催乳素和空腹胰岛素水平。

Results: Unlike Sc from pubertal rats, Sc from pseudopubertal monkeys constitutiely aromatized testosterone to estradiol and produced large amounts of lactate without FSH stimulation.

结果:与来自大鼠的Sc细胞不同,来自假性青春期恒河候的Sc在没有FSH刺激的情况下组成性芳香化睾酮生成雌二醇,产生大量的乳酸。

Objective : To observe the effect of gonadotropin - releasing hormone agonist on steroidogenesis of cultured human luteinized granulosa cells in vitro.

目的:观察促性腺素释放激素激动剂在体外对人卵巢黄素化颗粒细胞雌二醇(E2)和孕酮分泌量的影响。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后,在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素(hMG,0、0.1、0.2、0.5、1、2、5、10 IU/ml)单独或联合作用下进行体外培养,收集培养液,采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

These also were significant difference between different areas in Shandong province; for example, the highest mortality rate of the disease in Feicheng county was 41 times as the low death rate in Rushan county.

植物雌激素为杂环多酚类化合物,是一种具有弱雌激素作用的植物成份,主要包括异黄酮、木酚素和香豆雌酚三大类。

The radioactivity immune analysis was adopted to determine serum extradio-E2 (E2), progesterone and prolaction in Crab-eating Macaque after copulation.

方法]采用放射免疫分析法测定食蟹猴配种后血清雌二醇(E2)、孕酮和催乳素的水平变化。

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