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METHODS 3-methoxy-7α-methylestra-1,3,5(10)-trien-17β-ol was reducted with lithium and ammonia,then the intermediate was hydrolyzed in oxalic acid and reacted with malonic acid to get 3,3-dimethoxo-7α-methylestra-5(10)-en-17-ol(compound 4),which was oxidated by pyridium bichromate ,reacted with acetylene,and hydrolyzed in oxalic acid again to get the product tibolone.

方法以3-甲氧基-7α-甲基雌甾-1,3,5(10)-三烯-17β-羟基为起始原料经锂胺还原、草酸水解、缩酮化得3,3-二甲氧基-7α-甲基雌甾-5(10)-烯-17β-羟基(化合物4),然后用温和的氧化剂重铬酸吡啶盐氧化、炔化、再水解得目的产物。

Methods: RP-HPLC method was used to determine the content of diethylstilbestrol and testosterone in chick embryo.

目的:建立了RP-HPLC法测定鸡胚蛋及鸡蛋中己烯雌酚、丙酸睾酮激素残留。

Higher body mass index was significantly correlated with higher testosterone and lower total estradiol, estrone, progesterone and sex hormone binding globulin in midlife women.

在中年女性中更高的体重指数显著和更高的睾酮水平和低的总雌二醇、雌激素、孕酮还有,性激素结合球蛋白相关。

Bandicoot of SD of methodological general male 60, white group of random cent voidance, model group, Baoliezhi is treated contrast peaceful grain of group, front row is low, medium, tall dosage group, use ketone of third acerbity spermary hypodermic causes model of bandicoot prostate hyperplasia, after successive 30d giving drug, observe index of weight in wet base of weight of 6 groups of animals, prostate, prostate, prostate organizes ketone of Gao of configuration, serum , female 2 alcohol (the change of E2) level.

方法将雄性SD大鼠60只,随机分成空白组、模型组、保列治治疗对照组、前列宁颗粒低、中、高剂量组,采用丙酸睾丸酮皮下注射引起大鼠前列腺增生模型,连续给药30d后,观察6组动物体重、前列腺湿重、前列腺指数、前列腺组织形态、血清睾酮、雌二醇(E2)水平的变化。

Methods Blood samples were collected from the precaval vein of 6 female Tibet mini-pigs to test the concentration of sex hormones including T,P4,E2,FSH,LH,PRL and blood biochemical indexes including Ca, Mg, P, ALP, Cre, CHO, TP, and Alb during the estrous cycle.

对6头雌性西藏小型猪在发情周期内的发情当天、第5天、第10天、第15天、再次发情时分别采血5 mL并分离血清,应用放射免疫法测定血清睾酮、孕酮(P4)、雌二醇(E2)、促卵泡生成激素、促黄体生成激素、泌乳素;用自动生化仪测定血清钙、镁、磷、碱性磷酸酶、肌苷、总胆固醇、总蛋白、白蛋白。

Methods: A dual-chamber culture system was prepared. Porcine ovarian follicles were divided into healthy and atretic groups. Granulosa and thecal cells were isolated, and cultured with gonadotropin in the dual-chamber system. After 48 hours, angiotensin II, renin activity, estrodiol(E2), progesterone and testosterone in the media were determined by radioimmunoassay.

制备人胎盘羊膜双池培养系统,分离了猪卵巢颗粒细胞和卵泡膜细胞,并分为健康和闭锁两组,在促性腺激素刺激下协同培养。48 h后应用放射免疫测定法测定两种细胞培养液中的血管紧张素II、肾素活性、雌二醇(E2)、孕酮以及睾酮水平。

Methods 120 SD rats were divided randomly into blank group, model group, Baoliezhi control group and Qianliening granule groups with different doses. The models were established with hypodermic injection of testosterone propionate at 4 mg/kg after orchiectomy, at the same time, these rats were gastric perfused with different drugs respectively for 30 days. The body weight, prostate weight, prostate index, morphological changes, the level of T and E2 were measured. Results The body weight in model group was significantly lower than that in control group (P.05), the prostate weight and index were obviously higher than that in control group (P.01). The prostate weight and index were decreased significantly in Qianliening granule groups with meddle and high doses (P.01), the level of testosterone was increased significantly and E2 was decreased obviously in Qianliening granule group with high doses.

将雄性SD大鼠60只,随机分成空白组、模型组、保列治治疗对照组、前列宁颗粒低、中、高剂量组,采用丙酸睾丸酮皮下注射引起大鼠前列腺增生模型,连续给药30d后,观察6组动物体重前列腺湿重前列腺指数前列腺组织形态血清睾酮雌二醇(E2)水平的变化结果模型组大鼠体重较正常组明显减轻(P.05),而前列腺重量和前列腺指数明显升高(P.01);前列宁颗粒中、高剂量组的前列腺重量和前列腺指数明显下降(P.01);前列宁颗粒高剂量组可明显升高大鼠血清T水平,降低E2水平,使T/E2比例升高。

To observe the serum levels of thyroid hormones and sex hormones inmale patients with thyrotoxic hypokalemic periodic paralysis.

目的 观察男性甲亢性低钾周瘫患者血清甲状腺激素、黄体生成素、卵泡刺激素、催乳素、孕酮、总睾酮、雌二醇(E2)的水平变化。

Exposures to either E or MXC did not change sex ratio at birth, but the litter size was smaller in the former group; On postnatal Day 21, male pups exposed to either E or MXC at early pregnant stage exhibited shorter AGD than the controls, with the change most pronounced after MXC treatments. AGD in female offspring was unaffected after MXC exposures, but E treatments produced longer AGD in the females than that recorded in the controls; early pregnant exposures to E or MXC also accelerated sexual maturation as significantly more females exhibited precocious vaginal opening at weaning; In males, MXC exposure during early pregnancy decreased serum LH and FSH, but not testosterone levels.

但是雌二醇处理组雌性小鼠的AGD比对照组记录更长;③孕早期暴露雌二醇或MXC也加速性成熟,表现为更多的雌性小鼠在断奶时出现明显早熟的阴道开放现象;④MXC处理组的雄性小鼠可降低血LH和FSH但是不改变睾丸酮水平。

Immunocytochemical localization were investigated for the first time in the gonads, brain vesicle and nerve tube of Amphioxus using polyclonal antibodies against 17 b -estradiol, testosterone and progesterone receptors.

首次用雌二醇、睾酮和孕酮受体的多克隆抗体在文昌鱼性腺、脑泡和神经管中进行免疫细胞化学定位研究。

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