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雌酮

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(1) to deliver the Levonorgestrel (0.ml/kg,5mg/m) and Mifepristone gelatum (0.1ml/kg,5mg/ml) per cutem to the rat respectively and measure the serum concentration of levonorgestrel、mifepristone、estradiol and progesterone at different time by the means of HPLC, radioimmunoassay,chemical separation and atomic absorption spectrophotome.

大鼠皮肤涂抹米非司酮凝胶(0.1ml/kg,5mg/ml)和左炔诺孕酮凝胶(0.1ml/kg,1mg/ml)后的不同时间,利用高效液相色谱法、放射免疫分析法、化学分离与原子吸收分光光度法,测量血清中米非司酮浓度、左炔诺孕酮凝胶以及血清雌二醇、孕酮浓度。

New composite sorbents for separating estrone were synthesized with the monomers of acrylamide and p-vinylbenzoic acid using estrone as the template.

以雌酚酮为模板剂,以丙烯酰胺和对乙烯基苯甲酸为单体合成出用于分离雌酚酮的新型复合吸附剂。

Objective To investigate the effect of on serum sex hormone in mice with autoimmune thyroiditis.

目的 观察雌二醇、睾酮对自身免疫性甲状腺炎的影响和麻黄桃仁颗粒对雌二醇、睾酮水平的干扰。

Materials Methods: Beast ultrasound was performedduring luteal phase and late follicular phase in 50 women, aged 25~45, with normalmenstrual cycle and no distinct breast disease; Serum estradiol and progesteronelevel were measured by enzyme immunoassay on the day of ultrasound performed. Onehundred of twenty nine menopause transition women and 318 menopause women wereselected. All of them had ultrasound of breast and serum estradiol report. Breast pain wererecorded. Sixty menopausal women aged 56.8 yr. and menopausal aged 8.2 yr. participated1-year double-blind, randomized, placebo-controlled and 2mg 17β-estradiol continuouscombined with 1mg norethindrone acetate clinical study. Mastalgia was diaried. Breaststructure changes tested by high-resolution ultrasound at the 0 and 12 M.

资料和方法:征集身体健康,没有明显乳腺疾病的正常月经妇女53名,分别在月经的晚卵泡期和黄体期进行超声检查和测血清雌二醇和孕酮水平;收集有HRT前乳腺超声检查和血清雌二醇水平结果的绝经过渡期妇女129名和绝经后妇女318名,记录既往乳腺胀疼、过渡期乳腺胀疼、绝经后乳腺胀疼和既往乳腺病史。17 β-雌二醇2mg加醋酸炔诺酮1mg连续联合治疗和安慰剂随机双盲平行对照临床研究1年的60名参加者,分别在用药前后各进行一次乳腺超声检查,乳腺症状按同一表格由病人日记及经培训的一名医生定期随诊记录乳腺胀疼出现、消失的日期及持续的天数;超声检查用同一方法测量乳腺腺体层厚度、导管宽度、乳腺腺体结构及血流。

METHODS: Meibomian glands were obtained from young adult, ovariectomized mice that were administered 17beta-estradiol, progesterone, 17beta-estradiol plus progesterone, or vehicle for 14 days.

从已摘除卵巢的成年小鼠中获取睑板腺,这些小鼠分别接受了17beta-雌二醇、黄体酮、17beta-雌二醇联合黄体酮或载体注射14天。

RESULTS: The findings show that 17beta-estradiol, progesterone, or both hormones administered together significantly influenced the expression of numerous genes in the mouse meibomian gland.

结果:检查所见显示在小鼠睑板腺中,17beta-雌二醇、黄体酮或17beta-雌二醇联合黄体酮注射对于很多基因的表达都有显著影响。

We collected the urinary samples of one adult male, three pregnant females and one oestrus female.

我们收集了一只雄性黑叶猴不同阶段、三只雌性黑叶猴妊娠期和一只雌性黑叶猴发情期的尿液,用放射免疫分析法分别测定了雄猴尿中的睾酮、雌猴尿中的雌二醇和孕酮浓度,分析了不同阶段、不同状态下各种性腺激素水平的变化,并将其与黑叶猴的繁殖行为相联系,探讨笼养状态下黑叶猴性腺激素水平与繁殖行为的关系。

Methods Anhydrous acetic acid as solvent, 0.1 mol/L perchloric acid as titrant, crystal violet solution as indicator to establish non-aqueous titrations and ODS column as stationary phase, methanol and a mixture of 0.025 mol/L sodium phosphate monobasic and 0.02 mol/L sodium dedecyl sulfate (80:20) adjusted with phosphoric acid to a pH (4.8±0.1J as mobile phase, 220 nm as detective wavelength to establish HPLC-UV method for determination of piperazinylethylestrone drug substance; FMOC-CL as a derivatization reagent, FD as a detector to establish an HPLC method with derivatization and column switching for determination of piperazinylethylestrone in dog plasma.

分别采用无水冰醋酸为溶剂,0.1mol/L高氯酸为滴定液,结晶紫指示液指示终点的非水滴定法和HPLC-UV法测定哌嗪雌酚酮原料药含量;以FMOC-CL为衍生化试剂,采用柱切换柱前衍生化萤光检测的HPLC法测定狗血浆中哌嗪雌酚酮含量。

Also, the intensity of immuno-positive reactions to them enhance with the development of the gonads.

免疫组织化学法也显示卵原细胞、卵母细胞、精原细胞、精母细胞和精子细胞存在睾酮和17β-雌二醇的免疫阳性物质,而且睾酮和17β-雌二醇的免疫反应强度也是随着性腺发育成熟而增强。

Ovariectomies were performed in the OVX, OVX-E(OVX+nilestriol), L-ISO (OVX+soy isoflavones 30 mg*kg-1), M-ISO(OVX+soy isoflavones 60 mg*kg-1) and H-ISO(OVX+soy isoflavones 120 mg*kg-1) groups and animals in the SHAM group were subject to sham operation.

将10~12月龄的雌性Wistar大鼠随机分为6组:假手术组、切卵巢模型组、尼尔雌醇组、小剂量异黄酮组、中剂量异黄酮组、大剂量异黄酮组,每组8只。

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