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To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp,PCR testing technology were employed to test the Hu-IFN-α gene's genetic expression in its cross F1 and gynogenesis F1 generation.

为了分析外源基因在转基因鱼F1中的遗传特征和规律,采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1,雌核发育F1中的遗传表达情况进行检测。

In the present study, the artificial induction of homozygous gynogenesis and the inheritance and segregation of microsatellite markers in GDH in large yellow croaker was first reported, which will server as a foundation for rapid establishment of homozygous lines and making a genome map with GDH and homozygous lines in large yellow croaker.

首次报道了大黄鱼同质雌核发育的人工诱导及微卫星标记在GDH中的传递与分离,为大黄鱼纯系培育及利用GDH与纯系进行基因组作图分析等研究奠定了基础。

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp, PCR testing technology were employed to test the Hu-IFN-α gene's genetic expression in its cross F1 and gynogenesis F1 generation.

文章摘要:为了分析外源基因在转基因鱼F1中的遗传特征和规律,采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1,雌核发育F1中的遗传表达情况进行检测。

Results indicated that, just like the homologous sperm of summer flounder, the cryopreservative sperm of Lateolabrax japonicas could induce diploid gynogenesis of summer flounder.

实验表明:犬齿牙鲆同源精子经80 mJ/cm2 的紫外线照射可以完全失活,冻存花鲈精子经紫外线照射后也同样具有诱导犬齿牙鲆雌核发育的能力。

In this thesis, the integrated cDNA sequences of hira genes have been isolated from full-matured ovary SMART cDNA library of gynogenetic gibel carp and bisexual coloreded crucian carp by PCR and RACE-PCR.

本研究通过PCR和RACE的方法,分别从天然雌核发育银鲫和两性生殖彩鲫成熟卵细胞的SMART cDNA文库中克隆出hira全长基因。

There are indications that the peripheral blood cells of artificial gynogenetic grass carp can distinguish seven different cell types:erythrocyte, thrombocyte, lymphocyte, monocyte,neutrophilic granular cell, acidophilic granular cell and basophilic granular cell.

观察结果表明,雌核发育草鱼外周血液中可区分出七种不同类型的血细胞:红细胞、血栓细胞、淋巴细胞、单核细胞、嗜中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞,这些血细胞的显微及超微结构与已报道过的鲤鱼、鲫鱼、普通草鱼相应血细胞的结构基本相同

A common carp gynogenetic line including 44 individuals derived from the cross Barbless carp Hebao-cold tolerance red carp was used to construct a linkage map using 445 markers (265 AFLP markers, 127 SSR markers, 37 EST-SSR markers and 16 RAPD markers).

用265个AFLP标记、127个微卫星分子标记、37个EST-SSR标记和16个RAPD标记对大头鲤/荷包红鲤抗寒品系的F2雌核发育群体44个个体进行基因型检测,构建鲤鱼遗传连锁图谱。

This paper reports the results of preliminary study on the specificity of red blood cell antigens from varieties of carp (Cyprinus carpio L.).

用血球凝集和血清吸收试验的方法,对若干个鲤鱼品种及鲤鱼人工雌核发育系的红血球中的某些同种异型抗原纯合性进行了初步研究。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交,得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

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