雌核

Blood samples from 23 Huacaya alpacas ,3 males and 20 females ,were used to study chromosome karyotype ,so as to provide some effective cytogenetics basis for the selectioiymprovement by crossing and disease diagnosis of alpacas ,and genetic mechanisms of sex determination. Peripheral blood lymphocyte culture was used to prepare chromosome. Methods of trypsase-EDTA and BSG (Barium hydroxide / Saline / Giemsa)were used for G-banding and C-banding respectively . The results showed as follows:Firstly , the number of diploid chromosomes was in the alpacas 2n=74,with the karyotype 74, XY and 74, XX for males and females respectively.

为了从选种、杂交改良、疾病诊断以及性别决定的遗传机制等方面为羊驼的繁育与推广提供更为有效的细胞遗传学资料，本试验采用外周血淋巴细胞培养法制备染色体，分析了23只胡阿基亚型羊驼(Huacaya alpaca，雌20只，雄3只)的染色体及其核型，并通过BSG法(Barium hydroxide／Saline／Giemsa，氢氧化钡-盐溶液-吉姆萨，也即Sumner BSG法)与胰酶-EDTA法分别对羊驼的C-分带和G-分带进行分析，结果表明：(1)羊驼二倍体染色体数目为2n=74，雄性羊驼核型为74，XY；雌性羊驼核型为74，XX。

Observing the mature embryo sac of the mutant: It was found thatthe embyo sac appeared in typical polygonum type and more than 90% of the embryo sac hadintegral inner structure. They had egg apparatus, two polar nucleus and three antipodal cells , and their position in the embryonic sac was normal .

幼穗分化、成熟胚囊观察发现，90％以上的胚囊具有完整的内部结构，有卵器、两个极核及多个反足细胞，且位置正常，胚囊呈现典型的蓼型结构；同时还观察到雌、雄器官的发育是同步的。

The polar and egg nuclei of the female gametophyte are genetically identical.

雌配子体中的极核和卵核遗传上是同一的。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物，从猪卵巢上获得的卵母细胞数量虽然较多，但成熟度比较低；由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内，跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应；如果卵胞质成熟或活化程度不够，加之圆形未成熟精细胞成熟度比较差等原因，很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足，引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等，使ROSI显微受精失败。

The archegonial initials could be distinguished from the perip heral cells at the micropyle end of the female gametophyte before the completion of the wall formation where the cell wall had reached the center of the gametop h yte.

雌配子体中游离核之间细胞壁还未完全形成时，在其近珠孔端表面可看到颈卵器母细胞发生。

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明，枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成，是位于背部的一个囊状结构，它由两部分构成，前部的方形囊状体和后部的三角形囊状体，二者之间有一突起的脊；雌蛾静止时，腺体随第八和第九腹节一起嵌缩于第七腹节内，求偶时，腹部末端外伸，腺体细胞表皮外露，释放性信息素；腺体细胞呈方形，其表面覆盖几丁质表皮，表皮顶端无孔；细胞核很大，呈椭圆形，由双层核膜包被，位于细胞中下部，内有丰富的染色质；细胞质内有丰富的内质网，其内还有大量的大小不等的脂肪滴，以及线粒体和溶酶体等细胞器。

The results showed that the inheritance of gynoecious in cucumber was controlled by one dominant gene, and gynoecious was doninant on monoecious.

结果表明，黄瓜的全雌性由一对核基因控制，全雌对弱雌为显性。

The male and female rats (n=15, respectively) were randomly divided into three groups, and Fluoro-Ruby, a fluorescent tracer, was stereotaxically injected into the ventromedial hypothalamus, bed nucleus of the stria terminalis and posterodorsal nucleus of the amygdale by iontophoresis, respectively.Five days later, the rats were perfused, and the sections containing SDN-POA region and site of injection were cut on a freezing microtome.the sections were viewed to assess extent of tracer deposition in the injection site and to reveal the EGFP or Fluoro-Ruby fluorescence in SDN-POA under fluorescence microscope.

雌、雄性大鼠(各15只)随机各分成3组，分别通过离子电渗法将示踪剂Fluoro-Ruby注入下丘脑腹内侧核（VMH、终纹床核、杏仁核后背侧核。5 d后，灌流固定鼠脑，做冷冻冠状切片，荧光显微镜下确定示踪剂注入位置，并观察SDN-POA绿色荧光和Fluoro-Ruby标记荧光。

In order to establish mouse nuclei transfer technology, mouse zygote culture medium and activated conditions were optimized in vitro and enucleated method of MII oocyte were investigated in this study.

本研究通过对小鼠受精卵体外培养体系，MII期卵母细胞孤雌激活条件的筛选以及探讨MII期卵母细胞的去核方法为小鼠体细胞核移植的成功奠定基础。

In general, this malaria parasites in the mature or nearly mature erythrocytes, schizonts usually large, occurre in mature erythrocytes with more 20 merozoites most commonly arranged in mass.

两性配子体较大，均为长形，雄性配子体有驱核现象，并使寄主细胞膨胀变大；雌配子体较雄配子体长，有一定的包核现象。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失