雌核

It is the first time that these phenomena were observed: Two nuclei are surrounded by plasma and locate at the central of the female gametophyte and the others nuclei are positioned at the fringe of the female gametophyte; Before the pollen tube enters the female gametophyte. the nuclei in chalazal end begin splitting of plasma and form multinuclear cell.4. The pollens of Gnetumc are spherical or applanate with single aperture. The ornamentation of exine is spine. The basis part of spine is lenience and the top part of spine is tip or obtuse sphere.

首次在买麻藤属植物的雌配子体中观察到2个游离核位于配子体的中央位置，且被一团原生质所包围的，其余的游离核位于边缘的现象以及花粉管进入雌配子体前，合点端的核已经发生胞质分裂形成多核细胞的现象 4、买麻藤的花粉近球形或扁平型，有单萌发孔，外壁表面具小刺状纹饰，小刺基部宽大，末端尖或钝圆。

A common carp gynogenetic line including 44 individuals derived from the cross Barbless carp Hebao-cold tolerance red carp was used to construct a linkage map using 445 markers (265 AFLP markers, 127 SSR markers, 37 EST-SSR markers and 16 RAPD markers).

用265个AFLP标记、127个微卫星分子标记、37个EST-SSR标记和16个RAPD标记对大头鲤/荷包红鲤抗寒品系的F2雌核发育群体44个个体进行基因型检测，构建鲤鱼遗传连锁图谱。

The paper summarizes studies of in vitro inducement of haploids in cucurbitaceae, including anther culture, in vitro gynogenesis culture and induction of haploid obtained by irradiated pollen. The factors affectting in vitro inducement such as material genetype, medium, pretreatment, megaspores development stage and others had significant effects on the embryoids inductivities.

重点对葫芦科植物离体诱导单倍体的研究进行综述，包括花药培养、离体雌核培养、辐射花粉诱导单倍体培养等在育种中的应用，以及供体基因型、培养基、预处理方式、胚囊发育时期等因素对离体诱导再生频率的影响。

This paper reports the results of preliminary study on the specificity of red blood cell antigens from varieties of carp (Cyprinus carpio L.).

用血球凝集和血清吸收试验的方法，对若干个鲤鱼品种及鲤鱼人工雌核发育系的红血球中的某些同种异型抗原纯合性进行了初步研究。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

Three differential protein bands were revealed between the gynogenetic silver crucian carp and gonochoristic color crucian carp.

以雌核发育银鲫和两性生殖彩鲫的成熟卵为材料，分离卵壳，经处理得到卵壳可溶性蛋白组分。

This article conducts an optimum analysis of two technical treatments in the artificial female nucleus development of fish and makes the best selection of the techniques of irradiating time and irradiating conditions of sperms and the thermal shock of ovum chromosome redoublement so as to popularize and use the techniques in production.

本文对鱼类人工雌核发育中两个技术处理进行了优化分析；对精子的照射时间、照射条件和卵子染色体加倍的温度休克等技术进行了最佳选择。目的在于推广应用于生产。

Artificial female nucleus development of fish ; irradiate with ultraviolet ; induce chromosome redoublement ; thermal shock ; the best selection

鱼类人工雌核发育；紫外线照射；诱导染色体加倍；温度休克；最佳选择

Generally, two hybridization events have happened in the generation of gynogenetic crucian carp: the first one occurred about 6, 000 years ago when maternal ancestor of C. a. gibelio hybridized with males of C. a. auratus (ancestor of C. a. colored variety) and a diploid hybrid was produced; the second one happened over 10, 000 years ago, when the diploid hybrid was backcrossed with one of its parental population, by which the current triploid gynogenetic crucian carp emerged.

初步推断出东北雌核生殖银鲫是通过鲫鱼种群间的两次杂交产生的，第一次杂交是母本的银鲫同源种群与父本的彩鲫同源种群杂交，形成二倍体杂种；第二次杂交是二倍体杂种与两个祖先鲫鱼种群之一回交，形成了今天的三倍体杂种银鲫。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失