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Investigation on diploid androgenesis in fish〔J〕.

19〕 楼允东。人工雌核发育及其在遗产学和水产养殖上的应用

Zebrafish, androgenesis, female nucleus inactivation, UV, delayed fertilization

斑马鱼;雄核发育;雌核灭活;紫外线;延迟受精

The results indicated: 1) the rate of the PCR positive test for 1 120 transgene carps reached 60%; 2) the rate of the PCR positive test for 258 transgene carps' cross F1 generation reached 53.8%; 3) the rate of the PCR positive test for 36 transgene Carps' cytogenesis F

检测结果为:对1120尾转基因草鱼的PCR阳性检测,检出率为60%;258尾转基因草鱼杂交F1,阳性检测率为53.8%;36尾转基因草鱼雌核发育F1,阳性率为91.7%,说明Hu-IFN-α基因成功导入草鱼中,并能遗传给F1。

Based on the relationship between derivational rate of diploids an

研究结果为6-DMAP诱导栉孔扇贝雌核发育染色体加倍提供了一定的细胞学依据和技术参数。

In vitro gynogenesis is one of the major methods for haploid production.

离体雌核发育是单倍体诱导的主要途径之一。

ARTIFICIAL INDUCTION OF GYNOGENESIS IN CHINESE SHRIMP Penaeus chinensis Ⅱ.

中国对虾人工诱导雌核发育的研究Ⅱ。

It was a creative research method that by means of the culture in vitro of unfertilized ovary of strawberry to induce the gynogenesis and obtain the gynogenic plants with half number chromosomes.

首次对草莓未受精子房进行了离体培养,诱导了雌核发育,并获得了染色体数减半的胚囊植株。这是继草莓花粉植物后,开辟的获得草莓单倍体植株的又一条途径。

It was found that the polymorphism existed not only at the allele level but also at the locus level;many alleles of the microsatellite loci and some of the microsatellite loci had been lost during the process of artificial gynogenesis.

该实验结果还发现不仅草鱼的微卫星位点上存在等位基因的多态性,而且微卫星位点本身也存在多态性;在人工诱导草鱼雌核发育的过程中不仅存在微卫星等位基因快速丢失的现象,而且也存在微卫星位点丢失的现象。

The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles.

微卫星检测父本等位基因存在与否,以确定是否存在雌核发育。

Parthenogenesis in which the egg is activated by sperm ,but without fusion of the egg and sperm nuclei.

雌核生殖一种单性生殖,精子激活卵子,但是卵子和精子核没有融合

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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