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In vitro gynogenesis is one of the major methods for haploid production. There isfew report about physiological and biochemical changes of cucumber in vitro gynogenesis.

离体雌核发育是单倍体诱导的主要途径之一,有关离体雌核发育方面的生理生化机制的研究报道尚少。

The studies made know that Gynogenesis was a effective method to promote gene purification. microsatellite marker technique is an effective method of gynogenesis verification and genetic analysis.

研究表明雌核发育是促进基因纯合的一个有效途径,微卫星标记技术是是鱼类雌核发育鉴定和遗传分析的有效方法。

These results suggested that the examined meio-gynogenetic-2 group is a homozygous group and homozygous clone could be produced by continuous artificial induction of gynogenesis for two generations.

这些观察结果说明所检测的两代人工诱导雌核发育草鱼群体是纯合的,经连续两代人工诱导雌核发育可能建立起草鱼纯系。

Using the methods of classical shape size measuring and chromosome analysis, we studied the morphological characters and Karyotype of Cobitis sinensis in Xiangjiang River. We also investigated the evolution line and Karyotype polymorphism of the C . sinensis by the methods of cytological classification and cytocatalytic. Our results suggest that the formation of chromosome polymorphism was concerned with heterologous hybridization and some of C . sinensis may perpetuate in a clonal manner by natural gynogenesis.

本文以湘江流域的中华花鳅为研究对象,运用经典的形态测量观察方法、染色体制片技术对中华花鳅的形态生态特征、染色体组型进行了初步研究:运用细胞分类和进化方法对中华花鳅的核型演化和核型多态现象的形成进行了分析,提出:其核型多态的形成牵涉到鳅科鱼类的异源杂交,某些中华花鳅可能是行雌核发育生殖的。

However, whether these bands caused by recombination were hard to perorate owing to the analysis method restriction. 2. Isozyme mean proportion of polymorphic loci and the average heterozygosities of gynogenesis population in Paralichthys ohvaceu were 6.90% and 0.03 50 respectively.

牙鲆雌核发育群体同工酶的Ldh-C,Cat两个基因座位发生了重组,基因座位与着丝点之间的重组率分别为52.6%和29.8%;雌核发育群体RAPD分析发现136 个DNA片段中有22个发生基因分离,但由于研究方法的局限,不能确定是否发生基因重组。

Whole protein patterns during early stage of gynogenesis were first studiedthrough 2D electrophoresis. 11 specific protein dots were found, among which3(A1 ~A3 )were suggested to be the kind of inverse function factors which suppressgenes activating megagamete division, 8 (Bl~B8)were suggested to be closelyrelated with megagamet division activation.

通过双向电泳技术研究了黄瓜雌核发育早期的特异蛋白质表达,并发现了11个特异多肽点,其中3个(Al~A3)可能是开启分裂基因表达的一类反式作用因子,起抑制该基因表达作用。8个(B1~B8)可能是启动雌配子分裂密切相关的基因表达产物。

By means of hemagglutination and serological absorption tests, cross reactions between special antiserum and RBC of gynogenetic red carp F_2 and gynogenetic red mirror carp F_1 (RMGF_1) were investigated.

以抗雌核发育红鲤抗血清为试剂,三个雌核发育系R_(8305,R_(8413),RMGF_1的RBC反应值分别为1521—2360,1521—2028,853—1521,普通红鲤、镜鲤、白鲢的反应值在80—782。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要:用冷冻保存的花鲈精子作为异源精子,采用紫外线对精子进行照射使其遗传物质失活,然后与卵子进行"授精",可以刺激犬齿牙鲆鱼卵进行雌核发育,在受精后一定时间采用冷休克处理"授精"卵,抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

The main research achievements on oogenesis and embryogenesis in mammals are described in this review,including the resumption of meiotic division;activation of MⅡ oocyte;development of parthenogenetic,gynogenetic,androgenetic and reconstituted embryos;and the genetic and reproductive potentials of chromosomes within the first and the second polar body.

本研究将论述哺乳动物卵子发生和胚胎发生的最新研究进展,包括:减数分列的启动, MⅡ期卵母细胞的激活,孤雌胚、雌核胚、雄核胚和重组胚的发育以及第一与第二极体染色体的遗传和生殖潜力。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要:用冷冻保存的花鲈精子作为异源精子,采用紫外线对精子进行照射使其遗传物质失活,然后与卵子进行&授精&,可以刺激犬齿牙鲆鱼卵进行雌核发育,在受精后一定时间采用冷休克处理&授精&卵,抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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