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In order to the use of the male-sterility quickly, enriching the basic theories of male-sterility,we used the eggplant male sterile lines and their maintainers as materials, to analyze the physilolgical and biochemical characteristics and parthenocarpy by investigation of energy, substance and polyamine metabolism.

为了加快茄子雄性不育的利用进程,丰富茄子雄性不育的基础理论,本试验以雄性不育系及其保持系为试验材料,从能量代谢、物质含量、多胺代谢着手,对与茄子雄性不育性及单性结实有关的生理生化特性进行了分析,试验结果如下: 1 苗期不育系叶片中IAA和GA_3含量的不足,ZR的盈积以及ABA/(IAA+ZR+GA_3)比值的失衡导致了茄子雄性不育系苗期生长缓慢。

To our knowledge, reverse phase of high performance liquid chromatography was firstly developed to analyze the metabolites of 7-tocopherol with ideal results. Meanwhile, Gas chromatography/Mass chromatography were used to analyze the structures of unknown metabolites of 7-tocopherol and a main unknown metabolite was identified as a side chain ω- hydroxylate of 7-tocopherol.The kinetic results of of 7-tocopherol reacted in the S9 of male SD-rat liver showed that the optimal time was 45 min; the optimal concentration of S9 5 mg/ml; the optimal concentration of NADPH 1 mM; and the optimal concentration of 7-tocopherol 8 μg/ml. The result of γ-tocopherol metabolized in the S9 of male SD-rat was the best; male Brazil turtle better; and male cobia worst.

本试验首次确立了γ-生育酚代谢物的RP-HPLC分析方法,结果理想且重现性好;用GC/MS对γ-生育酚的未知代谢物结构进行分析,判断其中一种主要的代谢物为γ-生育酚侧链初级羟基化代谢物;γ-生育酚在雄性SD-大鼠肝S9的代谢动力学表明,本实验条件下代谢反应的最佳时间为45min,S9浓度为5mg/ml,NADPH浓度为1mM,γ-生育酚浓度为8μg/ml;发现γ-生育酚在雄性SD-大鼠肝S9中代谢最高,雄性巴西龟次之,雄性军曹鱼最低。

The Pu with breathed hard rock attacks to fall in his foot, face of gentle breeze Buddha, the soughing of the wind in the pines is chanted gently, brown bear is unavoidable some are actuation: Lose a hand and stand, look around all around, ask the world abandons me its who?

顽石无声的匍扑在他的脚下夜幕笼罩了寂寞空旷的大地,起伏的山峦与葱葱的树木都变得朦胧、虚拟起来。一只雄性的棕熊屹立在苍茫的天与地之间。顽石无声的匍扑在他的脚下,微风佛面,松涛轻咏,棕熊不免有些冲动:负手而立,环顾四周,问天下舍我其谁?

Leaves alternate; stipules lanceolate, 4-8 mm; petiole 2-11(-13) cm, pubescent or glabrous; leaf blade ovate, elliptic, oblong or subelliptic,(5-)9-21 × 2.5-10 cm, papery, slightly rough, secondary veins 3 or 4 pairs along midvein, abaxial surface strigillose or pubescent on veins, rarely glabrous, adaxial surface sparsely appressed strigillose or glabrous, base broadly cuneate, rounded, or obtuse, margin denticulate, apex acuminate or caudate-acuminate. Glomerules usually unisexual, sometimes bisexual, in the axils of current and fallen leaves along normal leafy stems, 4-10 mm in diam.; male ones on proximal part of flower-bearing branches; female ones on distal part.

叶互生 托叶披针形,4-8毫米;叶柄2-11(-13)厘米,被短柔毛或无毛;叶片卵形,椭圆形,长方形或,( 5-)9-21 * 2.5-10 厘米,纸质,粗糙的稍,次脉3或4 对沿中脉,背面具短糙伏毛的或短柔毛在脉上,很少无毛,正面疏生贴伏具短糙伏毛的或无毛,通常单性的团伞花序,有时两性,在正常生叶的茎的叶和脱落叶的腋内,直径4-10毫米;在忍受花的分枝的下部上的雄性的;在上部上的雌性的。

The amount of different mineral elements in different parts of male and famale B. thoracica had big difference, except Zn; the amount of other mineral elements in female breast muscle were all higher than that in male breast muscle, and the amount of Ca, Fe in leg muscle, Ca, Cu, Mn in skeleton and Cu, Mn in feathers of male were all higher than that of female, but the amount of Ca, Fe, Zn in feathers of female were higher than that of male.

雌雄个体不同部位、不同矿质元素含量存在较大差异,除Zn外,雌性胸肌中其他元素的含量高于雄性,雄性腿肌中Ca、Fe含量、骨骼中Ca、Cu、Mn含量、羽毛中Cu、Mn含量均高于雌性,雌性羽毛中Ca、Fe、Zn含量高于雄性。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术,研究了中华鳖不同组织Sox基因的表达,并通过PCR直接克隆法,分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中,Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达,而在肌肉、肝脏和雌性的卵巢中则无表达,显示该基因具有组织表达特异性克隆分析显示,在睾丸组织中表达的是TSSox1和TSSox4基因,而在脑组织中表达的是TSSox2和TSSox4基因,脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用,还可能在神经系统、免疫系统多种组织中起重要作用

For example, male-male copulations in locusts can be costly for the mounted male, and this cost may in turn increase selection pressure for males' tendency to release a chemical called panacetylnitrile, which dissuades other males from mounting them, said Bailey.

贝利说:&举例来说,雄性蝉之间的交媾对上位的雄性蝉来说代价昂贵,会付出生命的代价,而这种代价可能反过来增加了选择压力,促使雄性蝉倾向于释放一种称为panacetylnitrile的化学物,这种化学物会阻止其他雄性蝉,避免他们上位交媾。&

"For example, male-male copulations in locusts can be costly for the mounted male, and this cost may in turn increase selection pressure for males' tendency to release a chemical called panacetylnitrile, which dissuades other males from mounting them," said Bailey.

贝利说:举例来说,雄性蝉之间的交媾对下位的雄性蝉来说代价沉重,而这种代价可能反过来增加了选择压力,促使雄性蝉倾向于释放一种称为 panacetylnitrile 的化学物,这种化学物会阻止其他雄性蝉爬到自己身上进行交媾。

His concealed fangs and red chest say he's on top—at least until a male with bigger teeth or a badder attitude overthrows his regime.

他的隐蔽的尖牙和红色的胸部说他的处于优势,至少直到在一个雄性的和更大的牙齿或badder态度推翻他的政体。

In this paper, male sterile line of alfalfa was used to be improved with crossing, because the seed production of crossing is low. The affecting factors of seed production of male sterile line of alfalfa and its crossing progenies that are crossed with male sterile lines of alfalfa and good alfalfa that male is fertile were analyzed. The characteristics of female reproductive organs of male sterile line and its crossing progenies are analyzed in cell morphology. The genetic difference of male sterile line and its crossing progenies are analyzed in enzyme protein. The results showed: 1、Rate of podding of nature crossing and fecundity of artificial crossing of the male sterile plant BC1-2 that is the crossing progency of male sterile line of alfalfa and Medicago varia Martin.cv.'Caoyuan No.1'is higher than male sterile lines. Rate of podding of nature crossing and fecundity of artificial crossing of the male sterile plants BC1-19、BC1-21、BC1-22、BC1-23 that are the crossing progencies of male sterile line of alfalfa and Medicago sativa L.cv.

针对苜蓿雄性不育系杂交制种产量低的问题,采用杂交法对苜蓿雄性不育系进行改良,以原苜蓿雄性不育系为轮回亲本与雄性可育优良苜蓿品种杂交,分析苜蓿雄性不育系及其杂交后代种子产量的影响因素,从细胞形态学方面观察和分析杂交后代及原不育系在雌性生殖器官上的变异特点,从酶蛋白水平对杂交后代及原不育系的遗传差异进行分析,初步研究结果如下: 1、苜蓿雄性不育系与草原一号苜蓿的杂交后代不育株BC1-2,以及苜蓿雄性不育系与X′(苜蓿雄性不育系与新疆大叶苜蓿的杂种F1代)的杂交后代不育株BC1-19、BC1-21、BC1-22、BC1-23的天然杂交结荚率和人工杂交制种的结荚率和百粒重均高于原苜蓿雄性不育系。

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