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雄性原核

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METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要:通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中,然后将注射存活的受精卵植入假孕母鼠的输卵管;取其产3 wk龄子代鼠进行PCR初选,再通过Southern杂交进一步确证;利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

We infer that hira gene is related to sperm nucleus decondensation in fishes.

在果蝇中的研究已经表明hira基因与精核解凝形成雄性原核有关。

And the male pronucleus moved to the female pronucleus during the period of associating of the two pronucleus.

并且在系列的样品中可观察到,在雌雄原核联合的过程中具有雄性原核向雌性原核移动的趋势。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件,以Hprt为阳性对照,用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析,结果发现,转录Sry基因的睾丸组织以及雄性胚胎,从受精卵发育到囊胚的过程中,基本上不转录Xist基因;不转录Sry基因的雌性卵母细胞和雌性胚胎,从出现原核开始,到发育至2-细胞期的过程中,Xist基因一直不转录,但是,从4-细胞期开始,一直到孵化前囊胚阶段,雌性胚胎都转录Xist基因。

A total of 38 males and 24 femals irradiated at the pronuclear stage survived and were available for testing.

在原核期受照中共有38只雄性和24只雌性存活下来,并可供试验。

Most sperm nuclei eventually developed into the male pronuclei, just like the normal sperms, except sperm nuclei in a few zygotes kept dense throughout the fertilization process. Dense chromosome body was seen at the nuclear area during the prophase of first mitosis, and in the middle of spindle at metaphase of first mitosis.

精子入卵后形成精核,大多能够逐渐解凝、液化并膨大,最终形成形态正常的雄性原核,但在第一次卵裂早期退化成浓缩的染色质小体,并在胞质分裂时随机地分配到其中一个子细胞里的分裂沟附近。

推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。