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Napus and R. sativus and that the crossability was governed by the interaction of nucleus a...

通过胚珠培养,获得了甘蓝型油菜萝卜细胞质雄性不育系与萝卜的属间杂种。

In order to understand the effect of Ogura sterile cytoplasm on interspecific crossability between Brassica napus and Raphanus sativus, three radish cytoplasmic male sterilie lines of B. napus and their corresponding maintenance lines were used as female parents to hybridize with R.

用三个甘蓝型油菜萝卜细胞质雄性不育系及其相应的保持系作母本与萝卜杂交,以了解Ogura不育胞质对甘蓝型油菜与萝卜种间可交配性的影响。

Materials and Methods 20 cases of local ,healthy, crossbred pigs , including 9 males and 11 females ,their common bile ducts were ligated.

材料与方法健康杂种家猪20只,雄性9只,雌性11只,行胆总管结扎,建立梗阻性黄疸动物模型。

Using male sterile make crossbreed is the best way of hybrid seed production,and have achieved some success,but in seed yield and production of three-line there are still many problems and shortcomings,in particular, In China,the male sterile technology is still in the preliminary study stage.

采用雄性不育系生产杂交种是种子生产中最好的方法,一直受到苜蓿育种工作者的重视,并取得了一些成绩,但在种子产量和三系配套生产中还存在很多问题和不足,特别在我国,不育系杂交制种技术还处于初步研究阶段。

This male sterile gene can be effectively and rationally used in genus Brassica Oleracea and other Crucifer crops.

进一步揭示了甘蓝显性细胞核雄性不育发生的本质,为该基因的克隆、人工调控植物育性的表达及其分子育种提供了依据。

The results from laboratory and fields showed that "beiao" baits has coefficient of select food and after put in fields half year,and has value in controlling rats compareing with anti cruor bait and naturalness.

结论初步研究证明,贝奥雄性不育剂具有良好的适口性,有实际应用价值,在肾综合征出血热地区应用半年后,与自然对照和常规的抗凝血杀鼠剂比较

Disqualification --Unilateral cryptorchid or cryptorchid males.

失格:雄性单侧隐睾或隐睾。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

MATERIALS: Forty adult, white Kunming mice with 6 weeks old were randomly divided into cryptorchidism and control groups, with 20 animals in each group.

材料:6周龄雄性昆明白小鼠40只,随机分为隐睾组、正常对照组,20只/组。

It can be used for fresh-eating or processed into dried cuttlefish, frozen cuttlefish, frozen cuttle fish fillets, cuttlefish wire, and cuttlefish flower.

可加工成墨鱼干、冷冻墨鱼、冻墨鱼片、墨鱼丝、墨鱼花,雄性生殖腺和雌性缠卵腺可分别加工成干品"墨鱼穗"和"墨鱼蛋"。

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