难表达

Advance basic research has confirmed that the alkaloids extracted from thunberg fritillary bulb which is widely used in clinical medicine in vitro and in animal experiments have reversed MDR of leukemia. It will increase resistance of acute leukemia cells anticancer drug concentration and lower resistance the expression of Pgp. Pre-Clinical Experimental Research also shows that the conventional chemotherapy combined with the powder of thunberg fritillary bulb for the treatment of acute leukemia. Clinical complete response rate was higher, especially for patients with refractory or relapsed leukemia patients better clinical remission rate. In addition, Caladium particles Slices with chemotherapy in the treatment of RAL Clinical studies also show the Chinese refractory leukemia improve clinical efficacy safe and reliable.

先期基础研究已证实：临床常用的化痰散结中药浙贝母提取的生物碱体外及动物实验均有逆转白血病多药耐药的生物活性，能增加耐药的急性白血病细胞内抗癌药物浓度，降低耐药蛋白Pgp的表达；临床预实验研究也表明，常规化疗方案配合浙贝母粉用于急性白血病临床治疗，其临床完全缓解率明显高于对照组，尤其对难治及复发白血病患者临床缓解率更佳；此外，单药浙贝母颗粒配合化疗治疗RAL的临床研究亦显示中药在提高难治性白血病临床疗效方面安全可靠。

The findings don't mean that people with Type D personalities or people who have a tough time expressing their anger are doomed to be unhealthy, Denollet noted.

Denollet博士解释道，这些发现也不是说所有D型性格的人或者很难表达愤怒的人注定就不健康。

AbstractThough prokaryotic cells could hardly express recombinant human beta nerve growth factor with a proper threedimensional conformation, using of E.

基于原核表达系统极难表达具有正确三级结构的重组人β神经生长因子，E。

It is hard to express what a relaxing, special feeling it is to be bobbing buoyantly in a warm pool with a waterfall showering you whilst being gaped at by inquisitive Vervets.

很难表达心中的放松，特别的感觉，是要漂泊在暖池满怀希望的，而正在洗澡你目瞪口呆的好奇Vervets的瀑布。

I am a few words, but it doesn't mean I have thought, not romantic, doesn't mean I don't like it, just indrawn disposition constrains me, I do not speak or not mature, on behalf of my heart is not mature perhaps looks really is from me, I just difficult to express my true idea, I say, and I said, I often do not really thinking, if you really think I don't mature for you, I'd change can be affirmed is, I like you very much, very care about you and care about you, I hope you come back.

我这人个话不多，但不代表我没有思想，不够浪漫，不代表我不喜欢那样，只是内向的性格约束着我，我说话或做事不成熟，不代表我的心也不成熟也许从我外表看起来确实是那样，只是我很难表达出我心的真正的想法，我所说的和我说做的事往往不是我心里真正所想的，如果你真觉得我不成熟我愿意为你改变，可以肯定的是我非常喜欢你，非常在乎你以及关心你，非常希望你回来

In order to understand these differences on gene transcript level, even to get the genes and their expression related to cell culture, we researched the differences of total mRNA of these tissues by RAPD-PCR, the result showed that the difference between embryo and head of nymphae was much closer than embyo with others, which was accorded to the time order of insect upgrown, and the tissue which is more different from embryo on transcript level ismore diiBcult to be cultured in vitro.

四种组织的培养难易程度和分离出的细胞形态的不同，说明存在着组织表达的差异，为了从转录水平上来认识它们的差异，甚而获得与细胞培养难易相关的基因及其表达产物，本实验还采用了RAPD分析的方法进行了这四种组织：中后期虫卵胚胎、若虫头部组织、成虫的精巢和卵巢的mRNA表达差异研究，结果表明：从发育方向上看，四种组织的表达差异有分化越大差异系数越大的趋势，而在相同条件下则越难培养。

MethodsThe p73 gene ex- pression in 100 cases of AL including newly diagnosed and recrudescent or refractory ones and 20 normal controls was detected by relative quantitative reverse transcriptase polymerase chain reaction,and then the results were analyzed and compared by χ2-test,t-test and single agent variance. ResultsThe higher expression of p73 gene was found in 58% of AL cases.

采用RT-PCR方法检测100例初诊及复发或难治AL患者和20例正常对照者p73基因的表达，并采用χ2检验、t检验及单因素方差分析对结果进行统计学分析，以比较各组p73基因表达阳性率及表达强度差异。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

I find it hard to express my feeling.

我觉得很难表达自己的感受。

I suppose it is difficult for me to articulate but I have a fantasy for certain spaces, some are autobiographical and others are aspirational.

我想这是我很难表达，但我有一个一定的空间幻想，有些是自传和其他人的期望。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。