难获得的

In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

This can hardly be expected to be a peaceful process, and in fact it has been accompanied by major conflicts and social upheavals. The expansion of European capitalism in the nineteenth century involved the Opium Wars, genocide in the Belgian Congo, the Great Game in Central Asia, and many other forms of imperial conquest and rivalry. The seeming triumph of global capitalism at the end of the twentieth century followed two world wars, the cold war, and savage neocolonial conflicts.

很难想象这会是一个和平的过程，事实上它伴随着根本性的矛盾冲突和社会巨变：19世纪欧洲资本主义的扩张引发了鸦片战争，在比利时统治下的刚果实施了种族灭绝，在中亚展开大博弈，以及其他很多形式的帝国主义的政府和竞争。20世纪末全球资本主义看上去获得了胜利，其前有两次世界大战、冷战和野蛮的新殖民冲突。

It may be that, Li Si was the creation of a totalitarian bureaucracy to adapt to the principles of the ideology of the "power," Zhang was very difficult to maintain a balance, only to Confucian political theory and the principles of bureaucracy between the shoulder looking for a recovery co-ordination The new point of "duty."

或许可以这样认为，李斯获得了创立一种适应专制官僚制原则的意识形态的"权力"，张居正却只能在很难维持平衡的儒家政治理论与官僚制原则之间担负着寻找一个恢复协调的新的基点的"义务"。

To solve this problem, this paper proposes a method of retrieving Web problem resources based on ontology and description logics. It adopts OWL to express ontology and use RACER as a DL reasoner to fulfill description logic inferences.

Web上的题目资源大都以HTML页面的形式存在，由于从题目的语言表述中往往很难使机器获得其语义信息，包括所涉及的知识点、所采用的语言、难度等，因此

The government has crushed Falun Gong with more persistent and ruthless determination even than political opposition movements (before 1999 Falun Gong had no discernible political views, but its members abroad are now virulently anti-party). The clampdown makes it extremely difficult to gauge the sect's continuing support in China, but the anniversary will be a test of it.

政府镇压法xx的持久性和无情的决心甚至比政治异见人士还要大（在1999年以前，法xx没有清晰的政治观点，但是现在其在海外的成员是恶毒的反对方），由于该项取缔，很难估计法xx目前在中国获得多少支持，但是2009年的周年祭，将是一个测试点。

At the same time, overmany rituals and ceremony might raise repulsion and the individual hard to get the spiritual strength they want from certain form ritual.

同时，过多的典礼和仪式也会引起个体的反感，让他们很难在某些形式的典礼上获得他们想要的精神力量。

Shanxi's nationalisation drive will disappoint Chinese entrepreneurs, who have long hankered after bigger opportunities in industries, such as resource extraction, that are still dominated by the state.

山西的国有化政策让中国的企业家们难掩失望，他们长期一来都希望在资源开采这样的产业获得更多的准入机会。这些产业仍然被国有经济所控制。

It was showed that:ER65 had better sizing on second fiber difficult to sizing,the dosage of ER65 cut down by 20% in comparison with saponified rosin while by30% with ordinary dispersed rosin size.

试验情况表明，[1]乳液施胶剂ER65应用于难施胶的二次纤维，能获得良好的施胶效果；[2]在施胶效果相同的情况下，与皂化松香相比，采用ER65乳液施胶剂，可节约胶料用量的20%左右；与普通分散松香胶相比，施胶剂用量可以减少30%左右。

The ore of arsenopyrite is very easy to float, but hard to inhibit, the experiment creatively forward to the "Select I" in the middling re-election, and then incorporated into the final railings discharge, re-election of concentrate back to roughing of the closed-circuit process. Succeeded in separation the iron of marmites and arsenopyrite the concentrate grade obtained Zn 41.56%, recovery rate is 93.08%, comprehensive collect silver, gallium, indium and other rare metals.

毒砂可浮性很好，易浮难抑，本试验创造性地提出了精选I的中矿再选、再选尾矿并入最终尾矿排放、再选精矿返回粗选的新闭路流程，成功地实现了铁闪锌矿与毒砂的有效分离，获得了锌精矿品位41.56％、回收率93.08％的指标，同时综合回收了银、镓和铟等稀贵金属。

Other Valley climbers were understandably disappointed, and some of them who had planned to bag this first ascent themselves began to suddenly think seriously about the far higher, and more imposing, El Capitan.

其他谷地攀登者理所当然非常失望，计画要自己获得首攀的人中有些突然开始想要爬更难更不可能的El Capitan，这些不满却富野心的攀岩者中最有名的要算是Warren Harding。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。