难

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

A true graph of a stock, sunspot, or climate would include an axis for every influence, and would become an unpicturable thousand-armed monster.

所以说，无论是股票还是太阳黑子还是气候，如果真的要用图形来表示它，那么在这个图形中，每一个影响因素都应该有一个轴来代表它，结果就是这张图变成一种具有上千只手臂、难描难画的怪物。

A true graph of a stock, sunspot, or climate would include an axis for every influence, and would become an unpicturable thousand-armed monster.

所以说，无论是股票、太阳黑子，还是气候，如果真的要用图形来表示它，那么在这个图形中，每一个影响因素都应该有一个轴来代表它，结果就是这张图变成一种具有上千只手臂、难描难画的怪物。

Because of our hard body, mites, if he found one, you take a hanging in the wardrobe T-shirt, perhaps mite right your whole body is in you as a battlefield, kept biting, let your skin allergy, urticant painful area, and may give you have inflammation, swelling, thereby affecting the body healthy.

因蟎虫身体小，我们很难发现他的存在，假如有一天，你随手拿一件挂在衣柜T恤来著，也许蟎虫就马上布满你的全身，在你身上作为战场，不停地咬，让你的皮肤大面积过敏，瘙痒难忍，还有可能给你抓得发炎、红肿，从而影响身体健康。

Although already discovered feature of Conficker vermian part, but engineer of 360 safe centers also admits at the same time, at present very difficult still fixed position arrives this author,"If Conficker author does not have further action, that will track his true identity very hard, and once he starts attack, sequential and absolutely unimaginable."

尽管已发现Conficker蠕虫部分特征，但360安全中心工程师同时也承认，目前还很难定位到该作者，&Conficker作者如果没有进一步动作，那将很难追踪到他的真实身份，而一旦他启动攻击，后果绝对不堪设想。&

In such a restrictive society, it is difficult - if not impossible - for residents to get news of the outside world, and for the outside world to see in.

在一个如此充满限制的社会里，尽管可能，然而国民很难了解外面的世界，也很难被外面的世界了解。

Senior personage weighs bound of medium of a Shanghai, The level breakthrough that collective of this round of media appears on the market to be reformed in the industry is finite, this locality country endowment accuse will still be on dominant position for a long time: Internally, medium enterprise is appointed and dismissed in human affairs of company processing, tall canal wait for problem admiral to be taken off hard set pattern before; External, the administrative license that media manages is unlocked, mix course of study is managed and cross an area to hold concurrently and recombine to still see dawn hard.

一位上海传媒界资深人士称，此轮媒体集体上市在行业改革的层面突破有限，本地国资控股仍将长期居于主导地位：对内，传媒企业在公司治理、高管人事任免等问题上将难脱以往窠臼；对外，媒体经营的行政许可放开、混业经营和跨地区兼并重组仍难见曙光。

I. By the recommendation of preferential combos for restaurant, foreigners who feel difficult to choose restaurants under a foreign sky can easily find suitable restaurants and control budgets effectively.

一、 通过对餐饮店特惠套餐的推荐，让在异国他乡选餐厅难、点菜难的外籍人士可以方便挑选到适合的餐饮店，并能有效控制预算。

In Ting Chu to come a week ago, I began thinking about how to make Ting Chu tears in the program, and in some dialogue among friends, that is a very Ting Chu will ㄍ a ㄣ people do not like front of others to shed a tear, because he cried so long as one is difficult to stop, then heard the Ting Chu hard to cry the news, in fact, it is ignited the hearts of the raging flames, it is bound to accomplish this impossible task, to show I have not reached The purpose refuses to give up the spirit, wahaha!

在庭竹要来的一个礼拜前，我就已经开始思考要如何让庭竹在节目中掉眼泪，在一些友人的对话当中，得知庭竹是一个非常会ㄍ一ㄣ的人，不喜欢在别人面前掉眼泪，因为他只要一哭就很难停下来，当时听到庭竹很难哭的这消息，其实心中更是燃起熊熊烈火，势必要完成这不可能的任务，展现出我未达到目的死不罢休的精神，挖哈哈！

One may go wrong in many different ways, but right only in one, which is why it is easy to fail and difficult to succeed-------easy to miss the target and difficult to hit it .

Aristotle 4 犯错的方式有多种，但正确的方式却只有一个，这就是为什么失败容易成功难，不中标的容易，中标的难。——亚里士多德。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。