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Methods Seventy six aged patients with ostalgia caused by primary osteoporosis, whose T numerus less than certified value 2.5 were randomized into two groups: 1. Applying only CT (36 cases); 2. CT combined with Low frequency transduction pulsed electromagnetic fields (36 cases); Two groups simultaneously were given Calcium 600 mg and injected CT 50IU, ten days later injected 50IU every two days in two months, Low frequency transduction pulsed electromagnetic fields treatment was made by Tianjin Jinxitong company, frequency 20-40Hz, and treated 20 minutes by this machine, totally 30 days. Six months later we examined their Bone density again.

将存在骨质疏松性骨痛同时经DEXA骨密度仪检测T值低于标准值2.0以下的老龄原发性骨质疏松症患者76人随机分为两组:①单纯应用鲑鱼降钙素组(36人);②联合应用药物及低频电磁场组(36人),两组同时每日给予钙而奇D600片1粒,CT按每日肌肉注射50IU,10d后改为隔日肌注50IU连续应用2个月,低频电磁场治疗采用天津金希统公司生产的XT2000B型OP治疗仪,频率为20~40Hz,每天治疗20min,共治疗30d, 6个月后复查骨密度。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周,炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节,另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg,20分钟后踝关节照光,激光波长627.sum,功率密度 100mwcm',照射时间1000秒,能量密度100)/。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径,治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后,治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗,随机治疗一侧膝关节,另一侧作自身对照。兔耳静脉注入I'arrainrelomg/Kg,20分钟后,膝关节用金蒸气激光照射,激光能量密度100)儿旷。24 /J'时后取膝关节滑膜作病理检查,并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果: 1。模型观察:CIA大鼠炎症高峰期滑膜下炎细胞浸润明显,滑膜细胞明显增殖,炎症达到高峰后二周,血管缀形成,并侵蚀和破坏软骨和骨, CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生,滑膜下炎细胞浸润,也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明,各组织对HMME 的吸收速度和吸收量不同,荧光值一时间曲线不同,滑膜组织比皮肤和软骨对 HMME的吸收多,在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明:PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性,但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好,统计学检验差异有显著性。。3_军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少,图像分析结果表明面密度(阳性染色的面积总和与统计视野面积的比值)治疗组小于对照组,统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多,图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组,统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小,与对照组相比,统计学检验差异有显著性。结论:二。CIA、AIA的病理改变类似人类RA,可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同,滑膜组织比皮。

This paper is mainly comprised of two components:preparation of models of rats suffering from deficiency of spleen Yang;comparison of the pharmacodynamics impacts of different Lizhong Pills formulations made up by dried ginger or its different components in Lizhong Pills to the different groups of the models.1 Establish the model of deficiency of spleen YANG induced by " cypsum fibrosum and anemarrhenae " with complex factors1.1 ObjectivesWith using heat-clearing and fire-purging drug cypsum fibrosum and anemarrhenae,insteading of the common usage of rhubarb and folium sennae,accompanied with irregular diet and overstrained,the model of deficiency of spleen-YANG is to be established,which is much close to the pathopoiesis theory of " frigidity and chilly damaging yang,irregular diet injuring qi,overstrain impairing the spleen" in TCM.1.2 MethodsModel rats is fully fed every other day,given the molding drug of cypsum fibrosum -anemarrhenae(concentration 4:1) with 3 different frequency,and arranged to swim everyday in 25℃water until exhausted(performing as spitting bubble 3 times continuously or sinking unable to float by itself).

全文共分为脾阳虚模型的制备和干姜及其成分对理中丸药效学影响的比较两大部分。1&石膏-知母&复合法脾阳虚模型的制备1.1目的以清热泻火药石膏-知母代替常用的大黄、番泻叶类苦寒泻下药,并结合饥饱失常、劳倦过度的方法进行造模,以期建立更符合中医&寒凉伤阳、饥饱伤气和劳倦伤脾&致病思路的中医脾阳虚证候模型。1.2方法模型动物隔日足量喂食,每日在25℃水中游泳,直至大鼠连续三次吐泡下沉,或沉入水底,3秒内不能自行浮出水面,并以三种不同给药频率灌胃给予石膏一知母煎液,比较石膏-知母不同给药方法对制备脾阳虚模型的影响。

Peribulbar injection of dexamethasone 0.5mg was applied in the experimental group every other day 48 hours after operation to the end of the experiment, and the growth state of conjunctiva in both groups were observed. 2 HA implants from experimental group and control group respectively were extracted for testing the positive expression of VEGF by immunohistochemistry 3,7,14,21,28 days after operation.

术后滴妥布霉素滴眼液4次/d,实验组术后48h起隔日球周注射地塞米松0.5mg和实验结束,并观察两组的结膜生长情况;实验组与对照组分别于术后 3,7,14,21,28d摘取2只HA植入物行免疫组织化学检测VEGF阳性表达情况。

2 Any notice required or permitted hereunder shall be in writing and shall be deemed given as of the date it is: delivered by hand; by overnight or next day delivery; received by Registered or Certified Mail, postage prepaid, return receipt requested; or received by facsimile, as can be presumptively demonstrated by return fax or letter demonstrating successful facsimile transmission; and addressed to the party to receive such notice at the address and/or facsimile telephone number set forth below, or such other address as is subsequently specified to the notifying party by the receiving party in writing.

11.2 本协议项下所有通知应当以书面形式为之,并且在下列情况下视为已经交付:专人递送;经由隔日送达业务递送;由挂号邮件或保证邮件方式接受,邮资预付,需要回执;由传真接收,并回传真或信函证明已经成功发送;通知应当按照下列地址送交当事人,或按照下列电话号码传送,或者按照接受一方最近书面通知的地址或号码为准

Intragastric administration of attenuated salmonellae carrying HGF and GFP was performed on rats, each one with 0.2 mL, and once every two days for totally 3 times.

将携带HGF和GFP基因真核表达质粒的减毒沙门氏菌分别给予大鼠灌胃,每只0.2 mL,隔日1次,共3次。

Recombinant hFSH at a dose of 100 IU on alternate days the spermatogenetic process as evaluated by testicular cytologic analysis and an increase in the number of ejaculated sperm and inhibin B secretion.

此次随机单盲研究中,45位先天性低精子数量(0 x 106/mL),并且血浆卵滤泡刺激素与抑制素B浓度较低的男性患者,接受为期三个月的重组人卵滤泡刺激素50 IU或者100 IU隔日治疗,或者不采用任何治疗措施。

The rats in CpG and GpC groups were injected with CpG-ODN and GpC-ODN respectively every other day for 3 times, each at a dosage of 300 μg, and those in M group were uninjected.

注射抗-thy 1.1单克隆抗体后,隔天,CpG组经尾静脉注射CpG-ODN, 300μg/只,GpC组经尾静脉注射GpC-ODN,300μg/只,隔日注射,共3次。

All patients were treated with digitalis, diuretics and nitrate based vasodilating agents as fundamental management, and th...

所有病例均以洋地黄、利尿剂和硝酸酯类扩血管剂作为基础治疗,GH组加用rhGH 4.5IU ,第 1月隔日1次,第 2月每周 2次,第 3月每周 1次。

All patients were treated with digitalis, diuretics and nitrate-based vasodilating agents as fundamental management, and the patients of GH group were given 4.5 IU rhGH subcutaneously every other day in the first month, 2 doses every week in the second month, and then once a week in the third month.

所有病例均以洋地黄、利尿剂和硝酸酯类扩血管剂作为基础治疗,GH组加用rhGH 4.5 IU,第1月隔日1次,第2月每周2次,第3月每周1次。

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