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The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示,RSAP技术的引物为2条长度均为18bp的引物,引物的3'端为4~6个碱基的限制性酶切位点序列,接着是12~14个碱基的随机序列,2条引物的限制性位点和随机序列不同:PCR扩增的前5个循环采用35℃的退火温度,随后的35个循环采用48℃的退火温度;在25μL反应体系中,模板DNA用量为20ng,Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L,Tap DNA聚合酶用碳为1.5U,2条引物浓度均为600mmol/L;RSAP技术重复性好,适用性广泛,是一种操作简便、产率中等、稳定可靠的DNA标记技术。

That the electric elevator is that the call sign waits to work according to the outside as well as oneself controls law in fact, but call for is random , the electric elevator is that one people machine is each other dyadic in fact control system , the way the simple use sequential control or logic is controlled being that lift control system adopt the random logic therefore can not be satisfied with controlling call for's,controls.

实际上电梯是根据外部呼叫信号以及自身控制规律等运行的,而呼叫是随机的,电梯实际上是一个人机交互式的控制系统,单纯用顺序控制或逻辑控制是不能满足控制要求的,因此,电梯控制系统采用随机逻辑方式控制。

At the end of feeding for 2, 4, 6 and 8 wk, 6 rats in each group were randomly selected, the serum fasting blood sugar, fasting insulin and the fasting insulin resistance index, and the concentration of IL-18 in serum and liver tissue serosity were measured.

将48只♂SD大鼠随机分为正常饮食组及高脂饮食组,各24只,喂养至第2、4、6、8周末时, 2组各随机抽取6只,抽取门静脉血测空腹血糖、空腹胰岛素,计算空腹胰岛素抵抗指数;检测血清及肝组织匀浆IL-18浓度;光镜检查肝组织切片以评价肝脏脂肪变性程度。

They shuffle the deck, randomizing the cards before hand, so they can just pop cards off the top later and know that they're random.

他们洗牌,随机化之前,手中的牌,这样他们就可以只把最流行卡后,知道他们是随机的。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析,分别提取19份马铃薯品种的DNA,进行随机引物多态性扩增,从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究,每条RAPD引物扩增出4~9条带,共获得52条带,其中多态性条带为43条;19份马铃薯品种的遗传距离介于0.17~0.72之间,平均值为0.39,平均遗传距离介于0.31~0.51之间;聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类,聚类结果与系谱分析基本相符,同时也说明克新系列马铃薯的遗传基础有所拓宽。

Simple random sampling is the case where n objects are selected at random from a population, and each member of the population is equally likely to be included in the sample.

简单随机抽样是指从总体中随机取样n次,使得总体中的每个元素在样本中的出现的可能性相同。

In principle, layered random sampling and simple random sampling were used.

取样原则采分层随机抽样和简单随机抽样方式,共抽取12所学校,抽样得70个班级,含68位教师与其班级学生共754人,回收的有效样本共计70班,合计68位级任教师与739位学生。

The simple random sampling plan and stratified random sampling plan has been introduced and the application means and effects of which also has been presented,it has some characteristics such as material precision and obvious reduce of sample size,and rational advice has been presented.

介绍了简单随机抽样和分层随机抽样方法及其在检测中的应用方法和效果。

Consequently, investigated in detail are several concrete complementing schemes based on simple random sampling, stratified sampling.

摘要本文研究了不放回追加策略,包括基本设计和域追加设计都为简单随机抽样、分层随机抽样情形下不放回样本追加时域的估计的问题。

Methods Three towns of Wenjiang District were selected for study by computer simple random sampling.

方法计算机单纯随机抽取温江区3个乡镇作为调查对象,采用走村入户与农民面对面访谈的方式随机对温江5%参合农户户主用自制问卷调查表进行调查。分别采用Epidata 3.0和ISPSS 11.5软件进行数据录入和统计分析。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。