限定

This investigation include two parts: lThe primary culture, chondroblast induction and evaluation of MSC. 2The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan.

第一部分人骨髓基质干细胞的原代分离、体外培养、成软骨诱导及鉴定目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Some nouns can only be used in a singular or plural form depending on the collocate :...

这里的定冠词the是限定使用这些字母的单词的复数形式，懂吗？

As an experiential mode, Consociational Democracy points to four European countries (Austria, Belgium, Holland, Switzerland) and other developing countries(Luxemburg, Lebanon, Malaysia, etc) in some period in history. As an normative mode, it provides new norm for The Third World Countries. Its applicability depends on many favorable, unfavorable and uncertain factors.

作为一种经验模式，结盟民主主要是指奥地利、比利时、荷兰、瑞士四个欧洲国家以及卢森堡、黎巴嫩、马来西亚等发展中国家，且这些国家都有特定历史时段的限定；作为一种规范模式，结盟民主为第三世界发展中国家的政治发展提供了新的可供参考的模式，结盟民主在第三世界国家的适用性取决于多种有利、不利的乃至不确定性的因素。

It concludes that the essence of coprincipal lies in the fact that more than two doers regard acts of each counterpart as own ones and mutually utilizes, so as to form a uniform and organic unit and actualize component of special offence. So intention liaison is essential to coprincipal. But there is no reason to confine it to liaison of criminal intent, because it is joint act that is the central element of coprincipal. Intention liaison is only a coupling factor which facilitates joint act and explains whether act is joint. Provided that intention liaison can help to make more than two doers utilize and reinforce mutually so as to form into a uniform and organic unit, we will have full reason to regard the intention liaison as the one in terms of joint crime. Aggregation and filtration is essence of intention liaison.

关于共同正犯中意思联络的检讨，文章从个人责任原则如何在共同犯罪领域贯彻的角度出发，认为共同正犯的本质在于二人以上在具有行为相互认识的基础上，彼此将对方的行为作为自己的行为，并相互利用、相互补充，形成一个统一的有机整体，实现特定犯罪的构成要件，因此，意思联络是成立共同正犯所必需的，但是没有理由将意思联络限定于犯意的联络，因为行为之共同才是共同正犯的核心要素，意思联络只是促成并且说明行为是否共同的一个联结性要素，而不是刑法规范独立的评价对象。

I speak, of cours e, of free men who have a choice, not condemned criminals whose sphere of activities is strictly delimited.

当然，我说的是那些有选择权利的自由人，而不是那些活动范围受到严格限定的死囚。

Dielectric layer patterns which define recesses in which damascene interconnections are to be formed, are formed.

形成限定凹槽的介电层图形，在该凹槽中将形成镶嵌互连。

The positionable member defines an aperture extending through its midsection within which is disposed a depressible member.

可定位元件限定一个贯穿其中央部分的孔，在其内部设置一个可压下元件。

There are two ways to define a specialization: One is to reopen the namespace and add the definition of the specialization, which we can do because namespace definitions are discontiguous, Alternatively, we could define the specialization in the same way that we can define any namespace member outside its namespace definition: by defining the specialization using the template name qualified by the name of the namespace.

有两种定义特化的方式：一种是重新打开命名空间并加入特化的定义，可以这样做是因为命名空间定义是不连续的；或者，可以用与在命名空间定义外部定义命名空间成员相同的方式来定义特化：使用由命名空间名字限定的模板名定义特化。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。