限制性的

Old. In the nonrestrictive sentence, the store honored all the complaints, all of which were less than 60 days old.

在限制性从句中，商店只处理不超过60天的投诉；而在非限制性从句中，商店处理了所有的投诉，这些投诉都是60天内发生的。

When a nonrestrictive clause appears at the end of a sentence, place a comma before it and a period after it.

在限制性从句中，商店只处理不超过60天的投诉；而在非限制性从句中，商店处理了所有的投诉，这些投诉都是60天内发生的。

When a nonrestrictive clause appears at the end of a sentence, place a comma before it a period after it.

在限制性从句中，商店只处理8tTt8不超过60天的投诉；而在非限制性从句中，商店处理8tTt8了所有8 tt t8.com 的投诉，这些投诉都是60天内发生的。

The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示，RSAP技术的引物为2条长度均为18bp的引物，引物的3'端为4~6个碱基的限制性酶切位点序列，接着是12~14个碱基的随机序列，2条引物的限制性位点和随机序列不同：PCR扩增的前5个循环采用35℃的退火温度，随后的35个循环采用48℃的退火温度；在25μL反应体系中，模板DNA用量为20ng，Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L，Tap DNA聚合酶用碳为1.5U，2条引物浓度均为600mmol/L；RSAP技术重复性好，适用性广泛，是一种操作简便、产率中等、稳定可靠的DNA标记技术。

Methods Totally 200 cases of 9-12 yearold Han children without bone metabolism diseases were randomly selected. Questionnaire investigation and physical measurement of height and weight were made. BMD was measured by dual energy X ray absorptiometry. VDR gene polymorphism (cleavaged by restriction endonuclease of BsmⅠ and FokⅠ) was analyzed by PCRRFLP.

收集排除影响骨代谢疾病的西安地区9-12岁汉族儿童200例，进行问卷调查、体格测量；用双能X线骨密度仪测量腰椎BMD；用聚合酶链反应限制性片段长度多态性方法，分析VDR基因的2个限制性酶切位点BsmⅠ、FokⅠ的多态性分布。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

It also discusses some general problems of translating English postnominal modifiers into Chinese and then focus on the problems of translating English restrictive relative clauses, which include problems with the frequent use of de clauses, problems with the relative phrase, problems of translating non-restrictive relatives, and problems of translating non-restrictive relatives that modify personal pronouns.

在翻译问题上，文章集中讨论了英语限定关系分句的翻译，包括&的&分句的频繁运用的问题，关系短语的问题，非限制性关系词的翻译以及修饰人称代词的非限制性关系词的翻译问题。

With the growing trend of the economic globalization, the international technology trade demonstrates more and more significance, while the restrictive business practices resulted from it have seriously cumbered the development of international technology trade.

然而国际技术贸易中存在的各种限制性商业做法却阻碍了国际技术贸易的健康快速发展。上世纪九十年代以来以美国、欧盟和日本为代表的发达国家纷纷以各自的反垄断法为基础，并根据技术贸易的特点，制定单独的法规或法令，以期对技术转让中限制性做法予以规制。

In addition, based on the fact that phosphorus is usually the limiting nutrient for nitrogen fixers, a novel method named "nitrogen fixing bacterial growth potential" test, for evaluating limiting nutrients, was developed.

此外，根据固氮菌的主要限制性因子是磷这一特性，设计了一种被称为&固氮细菌生长潜力试验&的评价水体限制性营养因子的新方法。

In Chapter 2, the introduction of the dynamical models is presented, furthermore, on the basis of analyzing perturbation factors there are two dynamical models fit for the motion situation of the NEAs, which are imported as the restricted authentic particle model and the restricted mean particle model (with respect to the treatment of the orbits of the perturbing major planets) that the Post-Newtonian effect is also taken into account, while for the latter the influence of the 5/2 (or 2/5) resonant items of the interaction between the Jupiter and the Saturn is considered.

第二章是关于力学模型的介绍，在分析摄动因素的基础上，引入了近地小行星运动中比较合适两种力学模型，即限制性质点真实模型与限制性质点平均模型(这一平均指的是对摄动源大行星轨道的处理)，在两种模型中均考虑了后牛顿效应，另外在平均模型中还考虑了木星与土星之间的5/2(2/5)共振项的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。