限制性的

Their majestic repose and massiveness, the somewhat angular and flat elegance of their lines, the restraint shown in the treatment of the difficult material—their whole demeanor indicates a beauty of style that to us, who now can cut the hardest stone like cheese and bread, lacks necessity.

它们宏伟的静卧和重量，稍稍的倾斜和平坦的线段的高雅，对处置困难材料的限制性展示，整一个风度显示了在我们面前的风格的漂亮，那些人是现今能象砍奶酪和面包一样地砍硬石块的人，而实际上是没必要的

The peak of the bulge accorded with the vertical line through the posterior convexity of superior articular surface of atlas. The superimpose rate of the vertical line with the M line were 48% to left and 65% to right. The surplus existed in l～2mm to lateral of M line. The exit point of screw was the median point of vertical line on the anterior cortex of lateral mass. The superimpose rate of BB line the line linked with the

寰椎后结节中点及上关节突后凸尖下的骨质弧形隆起的顶点可作为寰椎椎弓根螺钉进钉点的准确标志，上关节突后凸尖及横突尖可作为寰椎椎弓根进钉点的大体标志；沿寰椎椎弓根轴线进钉较为安全，内倾17°，仰角变异大，出钉参照前结节顶点；椎动脉沟中外的厚度及进钉点处寰椎后弓的高度是寰椎椎弓根螺钉固定的主要限制性因素。

PCR-RFLP in FSHR gene 5'–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP

FSHR基因5'端（包括侧翼序列和第一外显子）的限制性内切酶TaqⅠ的PCR-RFLP标记研究发现，在秦川牛中双胎母牛的A基因频率（0.5500）和AA基因型频率（0.5000）分别高于单胎母牛的A基因频率（0.3500）和AA基因型频率（0.3000）；在黑白花奶牛中双胎母牛的B基因频率（0.5937）和BB基因型频率（0.6875）均高于单胎母牛的B基因频率（0.5416）和BB基因型频率（0.4167），也就是FSHR基因的5'端的PCR-RFLP标记在一个品种内单胎母牛和双胎母牛之间有一定趋势，但两个品种的趋势不同。

This article thinks that, as a criterion of liability fixation, strict liability does't think of the feasor's fault. It rests upon a factual test of causation that disregards proximate caused omissions.The defenses available in strict liability are strict limited.And finally,the unlawfulness characterizes is only the harm and not the activity producing it. As a category of tort liabilities, strict liability is a heterogeneous and multi-level continuum.

作为一种归责标准以及以之为据的责任类型，严格责任具有其独特的性质：在前者，严格责任具有价值判断上的非过错性、因果关系判断的模糊性、抗辩事由的限制性以及强调侵害行为的结果违法性；在后者，严格责任是一个不同质、多层次的连续体。

DESCRIPTION】the ingredients of the product comes from abyssopelagic fishes of special pure and clean sea areas, adopting Limited Enzymic Degraded Technology, a self-owned intellectual property, that is using compound bioenzyme preparations at a low temperature to extract active ingredients through a special low-temperature extraction process and specific sheared protein to ensure the bioactivity of Collagen.

本品原料来自纯净、无污染的特定海域中的深海鱼类，采用具有自主知识产权的限制性酶切制备多肽技术（Limited Enzymic Degraded Technology），在低温下使用复合生物酶制剂，通过特殊低温萃取工艺提取有效成分，通过特异性的剪切蛋白质，保证了胶原蛋白原有的生物活性，并将原分子量为30万左右的胶原蛋白分子降解成3500左右的胶原蛋白多肽（约含30个左右的氨基酸残基），其分子长度降至30—50nm，集中度达到99.5%以上，呈白色或淡黄色，无异味，无杂物，不添加任何激素和化学物质，没有任何毒副作用。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

The main characteristics include: the quiet Sun corona has a rapid response to the flare heating, within 2 second the corona temperature increases nearly one order of magnitude, but later such increase becomes very slowly; the downward velocity at early stage of impulsive phase is too small to be negnected; chromospheric evaporation made by conduction shows some intensely, the maximum evaporated velocity may be as large as 1000 km/s; the chromospheric condensation appears just when the chromospheric evaporation takes place and its place always coincide with that of transition region in the early stage; the maximum of chromospheric condensation occurs also in the early stage of impulsive phase, at which the density in condensation may be larger two order of magnitude than that of surround and the downward velocity may be as large as 100 km/s; after the maximum of chromospheric condensation, its strongth decreases gradually but its width becomes greater with time and have a tendency to transport into deep chromosphere; the front of chromospheric condensation heats atmosphere obviously and the temperature in the chromospheric condensation is higher than the original case; the downware velocity of transition region may be greater than 100 km/s at the early stage; when the evaporated material collide at the top of loop, the densityand the presure there increase rapidly, which result in the formation of a shock wave; and so on.

在改善色球辐射损失和精确分辨过渡区的基础上，通过联立求解连续性方程、动量方程和能量方程，构造了一维磁限制性耀斑环在热沉积作用下的大气动力学模型，获得了耀斑热模型下脉冲相早期较为细致的大气演化理论图象，其主要特征是：耀斑对宁静日冕的加热相当迅速，仅2秒钟日冕温度增加即达一个量级，之后日冕温度增加缓慢；脉冲相早期日冕向下速度很小，几乎可以忽略，热传导驱动色球蒸发，其过程较为激烈，蒸发极大速度可达到1000km/s；色球压缩区与色球蒸发几乎同时形成，且在早期其位置总是与过渡区位置重合；色球压缩极大发生在脉冲相早期，其时密度可高于周围约2个量级，向下速度可超过100km/s；色球压缩极大后，其强度逐渐减弱，但宽度明显增大，且渐渐脱离过渡区往色球传播；压缩区内物质温度要高于原先的大气温度；过渡区下降在脉冲相早期相当迅速，可超过100km/s；当蒸发物质在环顶高速相碰时，环顶的密度、压力迅速增大，且温度也有所增加，导致形成激波，其波面以每秒上百公里的速度自环顶向下传播；在脉冲相前期，日冕软x辐射对色球的加热作用可以忽略；等。

Then the author analyzes empirically China's terms of trade from 1981,and the causes of the change in the net barter terms of trade by means of regression analysis ,which can be quantitied.we get the results that China's net barter terms of trade had a declining trend while its income terms and factorial terms of trade had been improved,and the net barter terms of trade positively correlates with actual tariff rate,exchange rate and negatively correlates with GDP,besides,the factors which can not be quantitied such as pure price fighting,inappropiate tariff sytem,foreign-invested enterprises's tranfer pricing and region trade agreement also lead to the above trend of China's net barter terms of trade,and the change in China's net barter terms of trade had a neglect impact on international trade benefit.

得出中国价格贸易条件总体上存在下降趋势，收入、要素条件呈现改善的趋势；实际关税率、汇率与价格贸易条件存在正相关关系，GDP指数与其负相关。出口企业恶性价格竞争、不合理的关税体制、外资企业的转移定价、区域贸易集团是造成中国价格贸易条件下降的非量化因素。此外，本文分析了1981-2004年价格贸易条件变动导致的中国国际贸易利益变化，发现价格贸易条件变动对中国国际贸易利益产生了一定的负面影响。最后，本文在分析中国价格贸易条件变动环境的基础上，认为中国工业化阶段不高、贸易限制性政策实施空间的减小、&合成谬误&现象及知识经济时代的到来会在一定时期内对价格贸易条件变动不利。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

RESULTS: NK cells represent a unique subset of lymphocytes that have no restriction by MHC antigens and have the ability to lyses certain tumor cells without the requirement for prior immune sensitization of the host. In addition, NK cells can also produce cytokines and regulate the function of other immune cells. So, it is emerging to apply NK cells as therapeutic agents against a broad range of malignancies. In this respect, the anti-tumor activity of NK cells is the key factor of NK-cell-based immunotherapy.

结果：NK细胞是一种独特的淋巴细胞，对靶细胞的识别无MHC限制性，无需预先致敏即可直接杀伤肿瘤细胞，也可分泌细胞因子调节其他免疫细胞的功能，是机体天然免疫的主要承担者，也是获得性免疫的核心调节细胞，故NK细胞在抗肿瘤免疫中的地位越来越受到重视，随之增强NK细胞肿瘤杀伤活性的研究也逐步深入。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。