限制性的

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Authorize full or limited participation in proceedings before the court by interested persons or agencies, including appearance amicus curiae, intervention as a party pursuant to the Federal Rules of Civil Procedure, examination of witnesses or documentary materials, or participation in any other manner and extent which serves the public interest as the court may deem appropriate

授权利益相关的个人、机构全部或限制性地出席诉讼，包括协助法庭解释法律的人出庭，作为联邦民事程序规则规定的一方当事人的干预，文件材料的检查，或由法院认定的以其它方式和内容参加的诉讼。

Therefore,a sequence specific artificial zinc finger proteinis created when replacing the amino acids in the specific sites of C2H2 zinc finger protein and then fusing the protein to other activation or repression domains,while remaining the backbone of the zinc finger protein unchange...

ZFP可以介导靶基因的转录调控，抑制或激活特定基因的表达与配体依赖的靶基因激活或抑制；对DNA进行修饰，如人造限制性内切酶，重组酶，整合酶；抗病毒感染等。因此，人造锌指蛋白应用前景广阔，研究价值显著，是未来人类基因治疗的革命性的工具。

Lest these notes convey an impression that, unfamiliarity with the weapon is the only drag on greater use of the 75 by troops in the Korean fighting, it should be emphasized that the higher the ridge, the more prohibitive the weight factor becomes.

至少这些记录传达了一种印象：不熟悉75mm无后坐力炮的性能是妨碍士兵们在朝鲜的战斗中更广泛地应用这种武器的唯一因素。应当强调的是：山岭越高，武器重量对其应用的限制性就越大。

To restrict tying, the rule of patent misuse, Sherman Act, Clayton Act, 1995 Antitrust Guidelines for the Licensing of Intellectual Property are often used in USA. EU Guidelines on the Application of Article 81 of the EC Treaty to Technology Transfer Agreements (2004/C 101/02) analyzes the restrictive effect of tying, the effect of giving rise to efficiency gains and the pro-competitive effect.

搭售的法律规制，在美国有专利权滥用原则、《谢尔曼法》、《克莱顿法》、美国1995年《知识产权许可反托拉斯指南》；欧共体2004年《关于对技术转让协议适用欧共体条约第81条的指南(2004/C 101/02)》分析了搭售的限制性效果、提高效率的效果和促进竞争的效果。

To restrict tying,the rule of patent misuse, Sherman Act,Clayton Act,1995 Antitrust Guidelines for the Licensing of Intellectual Property are often used in USA.E...

搭售的法律规制，在美国有专利权滥用原则、《谢尔曼法》、《克莱顿法》、美国1995年《知识产权许可反托拉斯指南》；欧共体2004年《关于对技术转让协议适用欧共体条约第81条的指南(2004/C101/02)》分析了搭售的限制性效果、提高效率的效果和促进竞争的效果。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Field moisture capacity is the key factor not only resulting in afforestation success or failure, but also that impacting on productivity of stand at hot-dry valley of Jinshajiang River. It was concluded by the investigation that: the contour trench (40 cm×40 cm), mixted with can increase the field moisture capacity, in accordance, the productivity of stand could bue improved in some degrees. But bearing the weight of soil capability, survival individual plant on unit area was about 525 plant/hm^2. Further-more, introduction of the grade 1 seedling in afforestation could improve survival rate, and facilitate the stand productivity.

土壤含水量是影响金沙江干热河谷造林成败的关键因子，同时也是影响林分生产力的限制性因子，通过试验得出：采用水平沟整地(40cm×40cm)、与多年生小灌木混交，可在一定程度上提高林地土壤含水量，从而使得造林保存率和林分生产能力均有提高，但是受其土壤承载能力的影响，单位面积的保存株数保持在525株/hm^2左右；此外，用Ⅰ级苗木造林，不但可以提高造林保存，对林分的后其生长也有促进作用。

In the protein of barley, the contents of Glu, Pro, Phe, Arg are high, and the Limitation amino acids are Met and Trp. The content of Lys in barley is higher than that in wheat, which showed it had a good nutrition quality. The reason for barley protein unforms gluten net tissue was the content less of prolamine and glutein in the protein of barley. The one of main factors for barley showing high biological activity functions might lie in its high β-glucan and polyphenol chemical compound content.

大麦蛋白质中谷氨酸、脯氨酸、苯丙氨酸和精氨酸含量较高，限制性氨基酸为蛋氨酸和色氨酸，赖氨酸的含量高于小麦的，表现出较好的营养品质；大麦蛋白质组分中醇溶蛋白和麦谷蛋白含量较低，是其不能形成面筋网络组织的主要原因；大麦中含有β-葡聚糖和多酚类化合物是其生理活性功能较强的主要因素之一。

Tobacco industry's overseas sales grew almost 20 percent last year, and a nation of 350 million puffers adding five million more each year and few restrictions on advertising would make the People's Republic the jewel in the crown of global cigarette marketing plans.

美国烟草业的海外销量在去年上升了将近20%，中国有3.5亿的烟民，并在以每年500万的速度递增，尚且对烟草宣传的限制性措施极少，这使得中国成为全球香烟市场推广餐盘中的一块肥肉。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。