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Subclones used for synthesis of antisense RNA probes were generated and identified as PKCa and PKCP by partial sequence analysis, restriction mapping,and comparison with the published mouse PKCa (32) and PKC,8I (33) sequences.

亚克隆用于合成的反义核酸探针产生的,并确定为pkca和pkcp部分序列分析,限制性测绘,并与已公布的小鼠pkca ( 32 )和pkc , 8i数据库( 33 )的序列。

OBJECTIVE: To meet the needs of limited and high selective spinal dorsal rhizotomy, anterior and dorsal root of spinal nerve were microanatomized to be certain of the separation standard and the number of small nerve tracts, so as to provide reliable basis and novel operative standard for clinical operation.

目的:根据限制性和高选择性脊神经后根切断术的要求,对脊神经前后根进行显微解剖,确定神经小束的分束标准和数目,为临床手术提供可靠的依据和新的手术标准。

The greater the degree of exposure to silica and increasing length of exposure determine the amount of silicotic nodule formation and the degree of restrictive lung disease.

暴露在硅石中的程度和暴露时间的长短决定了矽结节形成的数量和限制性肺疾病的程度。

The present study has been conducted under the hypothesis that plants competes for limiting resources such as soli nutrients and sunlight and counted all individuals of each species in each permanent quadrate. There-fore, this study is different from the other works in hypothesis and methods.

而本文则在植物种间对土壤营养、光照等基本的限制性资源的竞争这一前提下,对样地中所有个体进行了统计分析,因而从研究的前提和方法上均与以前的工作有所不同。

The apoB gene polymorphism on Xba I site of 53 6 unrelated Chinese Han people,aged 25~64 years,was analyzed using PCR and Re striction Fragment Length Polymorphismmethod;serum apoA I,apoB,total chole s terol,triglyceride,HDL cholesterolwere measured,and LDL choleste roland non-HDL cholesterolwere calculated.

采用聚 合酶链反应结合限制性片段长度多态性分析的方法,分析536名25~64岁的无血缘关系的汉族人的apoB基因Xba I位点多态性,并测定其血清apoA I、apoB、总胆固醇、甘油三酯、高密度脂蛋白胆固醇,并计算低密度脂蛋白胆固醇LDL-C)及非高密度脂蛋白胆固醇。

Threonine is one of the essential amino acids for breeding pigs.

苏氨酸是猪营养需要的必需氨基酸,尤其是添加合成赖氨酸配制低蛋白质日粮时,猪日粮中的苏氨酸成为第一限制性氨基酸,可进一步改善饲料的氨基酸平衡和降低饲料的配方成本。

He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点,选择TJU103最大抑制效应浓度50μg/ml,与不同浓度CTLA4-Ig(1μg/ml、10μg/ml、50μg/ml、100μg/ml)联合,对供者T细胞的增殖抑制率分别为(59.5%、90.5%、93.4%、95.8%),TJU103(50μg/ml)与低浓度CTLA4-Ig(10μg/ml)联合,增殖抑制率在90%以上,已接近最大增殖抑制率,为二者联合应用的最佳组合,与单独应用组比较,有显著的统计学差异(p<0.05)。

I detest overly prescriptive diets that are impossible to follow, and the point was to eat more vegetables, fruits, legumes, and whole grains and less meat, sugar, junk food, and overrefined carbs, without giving up foods I loved.

本人痛恨限制性过强以至于难以执行的饮食计划。关键是在不放弃自己喜欢的食物的同时,多吃蔬菜,水果,豆类和粗粮;少吃肉,糖,垃圾食品,和过于精磨的碳水化合物。

Restriction map of H. polymorpha CBS 1976 chromosomal locus containing △9-fatty acid desaturase gene was constructed based upon the data of Southern analysis, and a 3. 4kb BamHⅠ-XhoⅠ genomic fragment was isolated. Sequence analysis showed that it contains an ORF of 1353bp with high homology with cloned yeast △9-fatty acid desaturase genes.

构建了H.polymorpha CBS 1976染色体△9-脂肪酸去饱和酶基因座位的限制性酶切图谱,进而分离了3.4kb BamHⅠ-XhoⅠ基因片段并进行全序列分析,结果表明这个片段含1个与已克隆的酵母△9-脂肪酸去饱和酶基因高度同源的、由1353bp组成的ORF。

Improved high ionic strength medium was adopted to extract the cpDNAs of Yunnan wheat,Tibet semiwild wheat and two cultivars of common wheat (Chinese Spring and Een 1).Restriction fragment analysis of cpDNAs was carried out with the use of 7 restriction enzymes.

亚种。我们采用改进的高离子强度法,提取云南小麦、西藏半野生小麦、普通小麦的两个品种(中国春和鄂恩1号)的叶绿体DNA,并用7种限制性内切酶对它们的叶绿体DNA进行了酶切图谱分析。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。