限制性的

The restriction enzyme patterns for eight species of mycobacteria were studied.

对PCR扩增片段的酶切样品的预处理和CE条件进行了优化，获得了8种分枝杆菌DNA的限制性内切酶谱图。

Among 90 positive clones in the 16S rDNA library of microorganisms, 23 OTUs were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, delta subclasses of the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Acidobacteria and Planctomycetes.

采用限制性内切酶HaeIII和RsaI对克隆文库中的90个克隆子进行了酶切分型，根据ARDRA酶切图谱的不同，可将其分为23个OTUs.16S rDNA序列分析结果表明，该克隆文库中主要包括变形菌门的alpha、beta、detla亚类、厚壁菌门、放线菌门、拟杆菌门、酸杆菌门及浮霉菌门等8类细菌及未培养细菌。

The original Standards Code extended the reach of GATT law in two ways, both of which continue on as part of the WTO single undertaking: Firstly, it established a new legal theory for complaints based on 'unnecessary obstacle to trade' which focuses on the inherent trade restrictiveness of a regulation.

原标准法扩大了关贸总协定法两种方式，双方继续就作为世贸组织单一承诺：第一，它设立了一个新的法律理论投诉基础上的不必要的障碍，两岸经贸后者侧重于内在贸易限制性规定。

The physical maps of mtDNAs from N.taihuensis Chen,Salvelinus taxa and Salmo salar were analyzed.Genetic distances between mtDNAs were calculated by the formula of Nei and Li(1979).Phylogenetic reconstruction method,UPGMA,was used to analyze the genetic relationships and the molecular dendrogram was established and revealed:the genetic distance between Salvelinus taxa and Salmo salar is 12.23% and that between N.taihuensis Chen and Salvelinus taxa-S.salar is 21.36%.Thisc indicates that Salangidae diverged from Salmoninae earlier.

比较了鲑形目3种鱼的线粒体DNA物理图谱，根据限制性位点差异法计算它们之间的遗传距离，利用UPGMA聚类分析法构建分子聚类图，结果表明：红点鲑属与大西洋鲑先聚在一起，再与太湖新银鱼相聚，前两者间遗传距离为12.33%，亲缘关系较近，太湖新银鱼与这两者间的遗传距离为21.36%，亲缘关系较远，表明银鱼科与鲑科分离较早。

The 682-bp fragment of human IFN-〓 cDNA was inserted into theXhoI site of the BMGNeo vector containing a neomycin resistancegene.The plasmid containing the translationally oriented IFN-〓cDNA insert (BMGNeo-IFN-〓) was selected by restriction enzymeanalysis.

将682bp的全序列人〓 cDNA插入到携有〓基因的表达载体BMGNeo的Xho I位点上，对此重组表达载体进行了限制性酶切鉴定。

Based on the scheme mentioned above, a restrictive partially blind signature is proposed and proved to be efficient, correct, unforgeable, as well as restrictive and partially blind.

在此方案基础上提出了一个高效的基于身份的受限部分盲签名体制，证明了该方案的正确性、不可伪造性、限制性和部分盲性。

The unmethylated probe can be cut in the recognition site by restriction endonuclease, causing the restoration of the fluorescence of TAMRA.

限制性内切酶可切割未发生甲基化修饰的探针，导致探针的发夹结构遭到破坏，引起TAMRA荧光信号的恢复。

According to the basic theory of market ecoomics and micro-economics,the marktstyle of irrigation sector has been identified as unperfected nature monopolize market,withthe characteristic of the same water being saled to different markets by irrigation waterprovider.

运用市场经济理论和微观经济学的基本原理，分析了灌溉水市场的基本特征，确定灌溉水市场为厂商向不同市场销售同一产品的限制性自然垄断市场。

Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes ( PLA2G4A, PLA2G4B and PLA2G4C genes) in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele or by conditioning on genotype with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy-weinberg equilibrium in both case and control groups.

采用聚合酶链反应-限制性内切酶片段长度多态性方法检测T2DM患者（病例组121例）和健康对照（对照组142例）3个cPLA2基因（PLA2G4A、PLA2G4B和PLA2G4C基因）的基因型；借助UNPHASED分析平台，利用等位基因条件分析和基因型条件分析方法分析基因的联合作用与T2DM的遗传相关性。

Methods We prospectively analyzed the results of pulmonary function tests in 158 patients with chronic bronchitis and emphysema,34 patients with restrictive ventilatory dysfunction and 34 healthy persons.

对158例慢性支气管炎和肺气肿患者，34例限制性通气功能障碍疾病的患者及34例正常人的肺功能进行前瞻性的分析研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。