限制性的

At the same time, the purified soil DNA was partially digested by enzyme Sau3AI, and the 2-6kb and 6-9kb fragments were recovered by dialyzing respectively.

纯化后的土壤DNA用限制性内切酶Sau3AI随机切割，以透析袋分别回收2-6kb和6-9kb的酶切片段，然后用T_4连接酶与上述脱磷载体连接。

Any parts of a plant which are found to have been discoloured orattacked by insects must not be used as medicinal herbs.

句中的从句无疑是限制性定语从句，但它有一半的状语性质。

Genomic DNA of maize was digested with restriction enzymes and hybridized with a mF2KP cDNA fragment.

应用不同的限制性内切酶消化玉米基因组DNA，以〓p标记的mF2KP cDNA片段作为探针进行Southern杂交分析。

With the development of internationalizing economy,the monopoly and limited commercial practice have more and more influence on the international economy.

随着经济国际化的不断发展，垄断和限制性商业行为对国际经济的消极影响愈来愈大。

Methods Using polymerase chain reaction-restriction fragment length polymorphism method, we detected 63 samples taken from 63 cases (including 36 invasive squamous carcinoma, 7 carcinoma in situ, 20 papillomas) for 248 point mutations in p53 gene.

采用聚合酶链反应――限制性片段长度多态性（polymerase chain reaction-restriction fragment length polymorphisms，PCR-RFLP）方法，对63例患者的送检标本（包括36例浸润性鳞状细胞癌，7例原位癌，20例乳头状瘤）中p53基因248位点的突变进行检测。

Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.

采用PCR技术和限制性内切酶片段长度多态性的方法，检测中国北方汉族168个核心家系的基因型。

Investigation and evaluation of soil and water resources in Minqin Oasis indicate that the proportion of grain crop, cash crop and grass is 36.94:45.16:17.90 for 2005. And a comparison with that for 1991 indicates that land use structure is developing towards rational proportion of grain crop, cash crop and grass; and harmony among agriculture, stockbreeding and forest. The main limiting factors of land resources are water, soil quality, salinity and fertility, and there is about 3.9218×10^8 m^3 water deficit per year in Minqin Oasis.

通过水土资源现状的分析评价，发现2005年民勤绿洲粮经草比例为36.94：45.16：17.90，与1991年相比，土地利用格局正向粮经草比例优化和农林牧协调方向发展；民勤绿洲土地资源的限制性因素主要为水分、土质、盐碱和肥力；对水资源现状分析发现民勤绿洲年缺水量达3.9218亿立方公尺。

Moreover, the modified AFLP method including all restriction enzymes, adaptors and primers has been optimized in this study.

此外，本研究对该方法的关键步骤，包括限制性内切酶的选择、接头与引物设计等，进行了优化。

These new types have replaced the original CPV type 2 worldwide, and CPV type 2b predominates overwhelmingly relative to CPV type 2a and is endemic in most populations of domestic canids in China.

通过单克隆抗体识别、限制性酶切及PCR等方法进行的流行病学调研表明：自从最早期的CPV-2型细小病毒出现以来，CPV已演化出新抗原型，即CPV-2a与CPV-2b型。

According to the porportion of restriction fragments shared,the genetic distancesamong the 4 populations were calculated with popgene3.2,and a cladogram wasproduced with MEGA3.1.The result showed that the genetic variance dismatch withtheir diapause variance.

根据限制性片段共享度，利用POPGEN3.2计算4个群体的遗似距离，并利用MEGA3.1进行聚类分析，结果表明其遗传变异与其滞育变异的特征不相符。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。