限制性的

The results showed that:There are 32 species,2 varieties,1 form of wild Hedychium plants in China including 2 new species,1 new form and 2 new-recorded species;Some species have specific breeding potential in view of the color of flowers,the type of fragrance and the height of plant in this genus;10 species have wide distribution volumes,but some species with high ornamental values are endangered;17 taxa were successfully introduced into Guangzhou;High temperature in summer is the most important factor to restrict the introduction of Hedychium to Guangzhou.

结果显示：我国野生姜花属植物有32种、2变种和1变型，共计35个分类群；部分种类在花色、香型、株高等性状上具有特异性育种资源；10个分类群野外蕴藏量大，有少数具有较高观赏价值的种蕴藏量小，处于濒危状态，亟待保护；17个分类群在广州地区露地栽培条件下成功引种；夏季高温是姜花属野生种在广州地区引种的主要限制性因子。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

Sinorhizobium fredii 042BS was isolated from root nodules of alfalfa from Xinjiang Region. Nodulation experiments showed that both soybean and alfalfa were nodulated by 042BS effectively.

费氏中华根瘤菌042BS分离自新疆的苜蓿根瘤，交叉结瘤试验发现它既可以在苜蓿上又能在大豆上结瘤固氮。16S rDNA PCR-RFLP分析表明，042BS与费氏中华根瘤菌模式菌株USDA205的4种限制性酶切图谱完全一致。

The cardiomyopathy was accompanied by multisystem abnormalities, including the nephrotic syndrome, bilateral sensory neuropathy of the hands and feet, bilateral carpal tunnel syndrome, hoarseness, hypothyroidism, splenomegaly, normocytic anemia, and thrombocytosis.

主治医生：张主任，荟萃该病例在临床，心电图，心脏超声上的发现都揭示出此病例是冠脉旁路移植手术后30个月病程中的进行性限制性心肌病，并伴有多系统功能异常，包括肾病综合症，四肢感觉障碍，双侧腕管综合症，声嘶，甲状腺机能减退，脾肿大，正细胞性贫血和血小板增多症。

Nodosus Pilin gene, the ovine IL-2 gene with synthesized primers with EcoR1 and BamH1 recognition sequence at 5'and 3'end respectively was amplified and cloned from the peripheral blood of ovine.

从健康绵羊血中分离白细胞，用TRIZOL试剂盒提取绵羊白细胞RNA，以RNA为模板，用设计OviIL-2的引物进行RT-PCR和PCR，扩增出设计有BamHl和EcoRl限制性酶切位点的绵羊白细胞介素2。

A SnaBⅠand NotⅠrestriction sites were introduced into NGF generespectively by PCR .After being amplified by PCR,this NGF genecontained SnaB Ⅰ and Not Ⅰ restriction sites at its 5'and 3'endsrespectively.

设计合成一对特异引物，分别带有 SnaBⅠ和 NotⅠ的限制性内切酶的识别位点，以 pPIC9K-NGF重组质粒为模板，用 PCR 方法扩增 NGF cDNA 片段。

The zymolytic cottonseed protein had higher solubility in pH 1-11, and it still maintained the acid soluble character.

溶解度分析结果表明，限制性酶解棉籽蛋白在pH 1~11范围内均具有较好的溶解度，仍然保持了棉籽蛋白酸溶性的特点。

Methods Polymerase chain reaction was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene.

应用多聚酶链反应技术扩增HPV16 DNA和Ha-ras癌基因相关片段，分别采用2％琼脂糖凝胶电泳和限制性片段长度多态性分析，检测口腔癌中的HPV16 DNA和Ha-ras癌基因第12位密码子的点突变。

Method］From January 2003 to January 2007, VVC arthroplasty was performed in 18 complex cases (19 knees),among them there were 11 cases with valgus knee,7 cases (8 knees) with ankylosed knee.

［目的］回顾性分析内外翻限制性假体（varusvalgus constrained implant，VVC）在复杂初次膝关节置换中的应用经验，总结VVC假体使用的适应证及临床效果。

With the growing trend of the globalization, the International Technology Trade demonstrates more and more significance, while the Restrictive Business practices resulted from it have serious cumbered the development of international technology trade.

然而，国际技术贸易中存在的各种限制性商业做法却损害了技术贸易的健康发展。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。