限制性的

And obtained recombinant plasmid PGEM-VP3 by linking prokaryotic expressionby vecto r PGEM-4T-1,and identified by PCR、double restriction enzyme analysis and sequence analysis,the result showed that it's true.

用EcoR I和XhoⅠ双酶切该片段并回收该片段，将此基因片段克隆至相同酶切回收后的PGEM-4T-1原核表达载体中，获得重组质粒PGEM-VP3，经PCR鉴定，限制性内切酶双酶切分析，证实了重组质粒的正确性。

The restricted edge connectivity problem of graphs and some other graph theories are all from the study of the big network design and reliability analysis.

图的限制性边连通度问题及许多理论都是源自大型网络的设计和可靠性分析。

In our living ,the restricted edge connectivity problem has a big application, and with the development of the field some scholars presented and studied a few restricted edge connectivity problems with different restrictions.

另外限制性边连通度在实际问题中有着广泛的应用，是图论研究中一个很活跃的课题，各类限制边连通度问题被相继提出并加以发展、应用。

Esfahanian and Hakimiproposed the definition of restricted edge connectivity.

最近，作为容错性的度量，Esfahanian和Hakimi提出了限制性边连通度的概念。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

MethodsOf 47 rat OLTs, 20 were per-formed with conventional two-cuff technique, 27 were combined with external bile drain-age and enterostomy. In the experimental group,a self-made connector was used to rebuid in vitro bile flow bypass, and all rats were placed in self-made restraining cages for bile collection.

47例大鼠原位肝移植，其中27例采用改进术式：在传统肝移植胆道外引流的基础上，加行空肠造瘘，并在体外建立胆汁回流通路，术后通过自制的限制性大鼠笼固定大鼠以便采集胆汁；其余20例仅单纯行肝移植术。

Characterizations of finite and recursively enumerable languages are obtained by asynchronous spiking neural P systems with extended rules.

由于在非同步模式下，考虑系统的计算时间是没有意义的，因此在非同步脉冲神经膜系统中只能定义非限制性语言。

Because for mankind, the sky is widened farsightedly, quiet and mysterious, the mere shadow fluctuates, it is overcast and fine and variable, but the psychology with strong restricted.

由于对于人类而言，天空开阔高远，静谧神秘，光影变幻，阴晴无常，但同时具有强烈的禁忌性限制性障碍性的心理暗示。

That sounds so simple, but the ambiguity is fiendish enough to keep the hounds confused.

听起来简单，但是这个模糊的限制性条款可以让最精明的编辑头疼不已。

He bypasses the Melian Inc Security Network this would have caused trouble for the lady, but, being foresighted, she'd included a get-out clause in the contract's small print: Does not apply to mortals being driven by Fate who are on their way to cause family rifts and binding chains of "living love and dying pain"( TM in the kingdom.

他迂回在美丽安交通安全网络公司保安网中这下那位女士可能有麻烦了，不过，她有预见能力，早就在合同的避难条款里，加了条限制性附属细则：不应用于被命运驱使的凡人，即就是造成家庭分裂，在王国之内有着'为求爱而死得很惨'

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐