限制性的

As with similar products, such assatellite TV receivers or cable modems, the Coactive Connector can be installed either by theconsumer or by a professional technician.

与同类产品，如卫星电视接收器或电缆调制解调器可由消费者或由专业技术人员安装的限制性连接器。

Restriction Fragment Length Polymorphism analysis of 16S rDNA sequence was used to reveal the diversity of culturable rare actinomycetes.

采用16S rDNA的限制性片断长度多态性(Restriction Fragment Length Polymorphism，RFLP)分析和序列分析，揭示其多样性。

Objective To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis and random amplified polymorphic DNA analysis for molecular typing of Neiss eria gon orrhoeae isolates.

目的 评价脉冲场凝胶电泳限制性内切酶分析及随机扩增多态性DNA分析对淋球菌分离株进行基因分型的能力。

To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis and random amplified polymorphic DNA analysis for molecular typing of Neiss eria gon orrhoeae isolates.

评价脉冲场凝胶电泳限制性内切酶分析及随机扩增多态性DNA分析对淋球菌分离株进行基因分型的能力。

To study genetic mutations of methylenetetrahydrofolate reductase C677T and cystathioinine-β-synthase T833C related to homocysteine metabolism in patients with ischemic stroke, the MTHFR gene C677T gene mutation and the CBS T833C gene mutation were detected by PCR-RFLP or ARMS method in 74 patients with ischemic stroke and 83 normal people for control. Results showed that the frequencies of MTHFR genet homogenetic type(2.7%), heterogenetic type(51.4%) and T allele(28.4%) in ischemic group were higher than those in control group(1.2%, 39.8% and 21.1%, respectively).

为研究同型半胱氨酸代谢相关酶亚甲基四氢叶酸还原酶基因C677T和胱硫醚-β合成酶基因T833C位点碱基突变与缺血性脑卒中的关系，对74例缺血性脑卒中患者和83例健康对照者，采用聚合酶链反应-限制性片段长度多态性技术检测MTHFR基因C677T基因型，用扩增阻滞突变体系法检测CBS基因T833C突变。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

Restriction sites polymorphisms of rRNA gene for Leptospira can be used to classify and identify Leptospira in China and also to study on molecular epidemiology for leptospirosis.

rRNA基因限制性内切酶酶切位点多态性可用于钩体分类，而且是钩体病分子流行病学调查的一种好方法。

The results showed as follows: there was a certain limitary role of specific amino acid default on the growth of R.amylophilus, B.fibrisolvens and P.ruminicola.

结果表明：特定氨基酸缺省对嗜淀粉瘤胃杆菌、溶纤维丁酸弧菌和栖瘤胃普雷沃氏菌的生长限制性作用有所差异。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

The use of "speculative fiction" in the sense of expressing dissatisfaction with science fiction was popularized in the 1960s and early 1970s by Judith Merril and other writers and editors, in connection with the New Wave movement.

超过百分之九十的现代推理小说都采用同一种视角：限制性第三人称视角&，这里把&Speculative fiction&翻译作&推理小说有些不同看法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。