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Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短,构建成表达载体,在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白,当其生色基因中 65位 Ser点突变成 Thr,则 GFP的激发波由 396nm增至 4 70 nm,不用 UV激发,仅在自然光源下就可看到强烈的绿色荧光,发光强度增加 6倍,若从 3′端截短 12个氨基酸密码,对发光无影响,但截短 75个氨基酸密码,则激发波长降到 360 nm,发光强度减少 12倍。

Methods From April 1977 to December 2004,7 patients were admitted. There were 4 false aneurysms in aortic isthmus, and 1 right subclavian artery pseudoaneurysm, and 1 right axillary artery false aneurysm, and 1 thoracicoabdominal multiple pseudoaneurysms.

方法1977年4月~2004年12月收治的7例白塞氏病所致动脉瘤患者中,主动脉弓降部假性动脉瘤4例,右锁骨下动脉假性动脉瘤1例,右腋动脉假性动脉瘤1例,胸腹主动脉多发性假性动脉瘤1例。

Studied in the paper are the performance, separation efficiency and pressure loss of a uniflow square shaped cyclone separator which can be directly and easily installed on the water wall of a circulating fluidized bed boiler.

对可与循环流化床水冷壁直接结合而易于布置的下排气方形旋风分离器的性能指标:分离效率、压降等进行了研究。

To prevent possible danger to the wind turbine struc-ture, it may be advisable to suppress the automatic untwisting at extremely high wind speeds (e.g. V ≥ 0.8 * Vref) and to have the yaw system control the plant automatically until these wind speeds have dropped to an acceptable level.

为防止风机结构上可能的危险,建议在风速极高情况下(如:V≥0.8*Vref)抑制解缆操作,且应使偏航系统自动控制机组,直到风速降到一个可接受的级别。

Calculated flux distribution of the upfeed system in the household hot water heating.

介绍了分户式热水供暖系统的水力工况原理,通过分析分户供暖系统的水力特点,应用不等温降的管网水力平差的计算方法,建立了分户供暖管网系统水力分析的数学模型,并应用该模型对工程中习惯采用的下供下回式分户热水供暖系统进行了分析,编制了水力分析软件。

Introduced the principles of hydrodynamics in the household hot water heating system,Analyzed characteristics of hydrodynamics in the household hot water heating system,using unequal temperature reduction hydraulic balance method,established hydraulic analysis mathematics model for household hot water heating system.Using the model analyzed the upfeed system of household hot water heating in engineering.

介绍了分户式热水供暖系统的水力工况原理,通过分析分户供暖系统的水力特点,应用不等温降的管网水力平差的计算方法,建立了分户供暖管网系统水力分析的数学模型,并应用该模型对工程中习惯采用的下供下回式分户热水供暖系统进行了分析,编制了水力分析软件。

Objective To investigate whether functional abnormalities in coronary vasomotion are present in asymoptomatic patients with slight hypertension.

应用经胸超声心动图冠脉血流显像技术测量 2 3例无症状轻度高血压患者在静脉输注潘生丁溶液结合等长握力负荷状态下前降支冠脉血流的速度变化,并与 2 1例正常健康者对照。

And Molly won seven shillings on a three year old named Nevertell and coming home along by Foxrock in that old fiveseater shanderadan of a waggonette you were in your heyday then and you had on that new hat of white velours with a surround of molefur that Mrs Hayes advised you to buy because it was marked down to nineteen and eleven, a bit of wire and an old rag of velveteen, and I'll lay you what you like she did it on purpose...

摩莉把赌注下在一匹名叫&永勿说&的马上,赢了七先令。然后坐那辆有五个座位的双轮破旧马车,沿着福克斯罗克回的家。当时你可风华正茂,戴着镶了一圈鼹鼠皮的白丝绒新帽。那是海斯太太劝你买的,因为价钱降到十九先令十一便士了。其实就是那么一点铜丝支着一些破破烂烂的旧丝绒。我敢跟你打赌,她准是故意的

In addition, because gold and silver ink of qrganic is unstable and the transfer of and sent, in wet waterish "" status under business card printing and membership card must be produced to reduce the business card printing and membership card making machine speed, greatly influenced the polychromatic machine productivity.

此外,因为金、银油不朱的化学本质不安定,转印性又不对,在&湿压湿&的状况量下制卡和会员卡制息不离务必降矮制卡和会员卡制息机的速量,这样又不小不小感化了众色机的不入产不败果。

This is economy constrictive period next whole worlds fall firewood spreads wetly.

这是经济紧缩期下全球降薪潮的蔓延。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。