降下

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

ABSTRACT Aiming at problems of trans-vector controller in a vehicle-carried induction motor such as variable rotor and stator resistance following motor temperature, and their inductance varying with flux saturation degrees. Firstly, CSI and VSI are introduced, for CSI, it can hold the advantages both FOC and DTC using stator flux directional, PI parameter and simulation are also introduced. Secondly, equations of IM in the synchronous rotary frame and its static counterpart, as figuring in the saturation condition of magnetic field, are dealt in this paper and the simulation results are presented. Thereafter, the parameter designing of PI trans-vector controller is processed, which features the non-linear model. In practical realization, as neglecting the dynamical change of motor inductance, this paper employs online modification of inductance parameters, according to the flux saturation, then transforms the non-linear equations into linear ones, and so compacts the structure of controller. Thirdly, in view of the maximum torque output is extraordinary requisite when Electrical Vehicle start up, accelerate, and overtake, this paper issues the maximum torque when both inverter and battery capacity is corporeally definite. Fourthly, on-line differentiating and analyzing both rotor's time constants and its flux by using the expanding order reduction and discrete Kalman filter equation are fathomed, in order to realize high-performance trans-vector algorithm, and also the simulation results are presented. Fifthly, the close-loop regenerative brake system, when the EV's torque is set constant, is deduced; at the same time, unification of electric drive model and brake model is executed. Combined with maximum charge current demands, the mathematical model for online yielding torque as a demand.

本论文针对车载异步电机在矢量控制器所遇到的问题：定转子电阻随温度变化及随电机饱和程度变化的转子电感及定子电感而影响电机调速性能提出一套完整的解决方案：分析了电流型逆变器和电压型逆变器在实现矢量控制时控制器参数的计算，分析出对于电流型逆变器在采用定子磁场定向时，其性能同时具有矢量控制和直接转矩控制两者的优势，并对电压型逆变器在转子磁场定向下的模型进行了仿真研究；建立异步电机非线性模型，推导出考虑磁饱和时异步电机在同步旋转坐标系及静止坐标系下的方程，并做出了仿真结果，建立异步电机在非线性模型下的矢量控制调节器的PI参数设计，在实际应用中，若忽略电机电感的动态变化，可以根据当前的磁路饱和状态而在线修正电感参数，从而将非线性方程线性化，降低控制器的复杂度；考虑到电动汽车在起动和加速超车时需要电机有最大转矩输出，本文讨论在逆变器容量一定和电池供电能力有限的情况下电机最大转矩输出问题；推导了异步电机扩展降阶、离散卡尔曼滤波方程在线辨识转子时间常数和转子磁通，用于实现高性能的矢量控制算法，并给出了仿真结果；推导出了电动汽车恒转矩给定的闭环回馈制动系统，实现了电动控制模型和制动模型的统一，而且结合铅酸电池最大充电电流的要求，为制动转矩在线给定建立了数学模型；设计了基于双DSP系统的高性能矢量控制器软硬件框图，并以大量实验数据说明矢量控制在电动汽车应用的实际应用状况。

In the same way, a Four-coloring of a maximal planar graph of 25 order G(subscript M25), is obtained with "simplified method of reduction of order".

利用"简化降阶法"对一个一定拓扑结构的12阶最大平面图G(下标 M12)进行着色，得到G(下标 M12)的四色着色方案；以同样的方法对一个一定拓扑结构的25阶最大平面图G(下标 M25)进行着色，得到了G(下标 M25)的四色着色方案。

Objective To investigate the application of anterolateral thigh fasciocutaneous flap on abdominal chronic ulcer,and provide evidence for its clinical use. Methods From Oct 2005 to Dec 2008,5patients with abdominal ulcer caused by different reasons were repaired with anterolateral thigh fasciocutaneous flap, the flap pedicled with rami descenden of lateral circumflex femoral artery was designed according to the size of abdominal defect,the vessel pedicle was dissociated adequately, the flap was transferred to the abdominal wound under appropriate tension through tunnel, the donor site was sutured directly or repaired with skin-grafting. Results The anterolateral thigh fasciocutaneous flap survived well in all 5 patients,there were no other complication such as skin necrosis.

目的：探讨股前外侧皮瓣在下腹部慢性溃疡中的作用，为其临床应用提供依据；方法：2005年10月～2008年12月间，对5例不同原因引起的下腹部慢性溃疡患者进行修复，术前根据腹部组织缺损的大小设计以旋股外侧动脉降支为血管蒂的岛状股前外侧皮瓣，充分游离血管蒂部，在合适张力下经过皮下或开放隧道将皮瓣带蒂转移至下腹部创面，供区直接缝合或植皮修复。

Along with seawater stress strength upward, the ratio of root to shoot, Na+, Cl- and soluble sugar contents were increased significantly. Under the 10% and 20% seawater treatments, chlorophyll contents have no obvious difference compared with the control, but significantly decreased when the seawater stress further increased. Under the 10% seawater treatments, K+ contents in the shoot and root of Lactuca indica seedlings have no distinct difference compared with the control, but along with seawater stress strength upward, K+ content were markedly decreased by degrees. However, soluble protein content first increased then decreased with seawater concentration augment.

结果表明：苦荬菜幼苗地上部受海水胁迫较为显著，而根在30%海水以内与对照相比没有显著差异；根冠比随着海水比例的增加而提高；10%和20%海水处理下，叶绿素含量与对照相比差异不显著，但随着海水浓度的进一步增加，叶绿素含量显著下降；10%海水处理下，苦荬菜地上部分及根K+含量与对照差异不显著，高于10%海水处理，K+含量随海水比例的增加地上部和根部均逐渐显著降低；海水处理下，体内Na+和Cl-含量逐渐显著增加；地上部可溶性糖逐渐显著增加，而可溶性蛋白呈先升后降。

Theresults indicated that the initial desorption rate of As was higher than As, but thefinal desorption percentage of As was two times higher than As; that in therange of pH(6.5-8.5), the desorption of As was the lowest（pH=6.5）,and thepercentage increased with the pH value increasing or deescalating, but for the As,thepercentage increased with the increase of pH; that the supersonic accelerated desoptionof arsenic and the final percentage was lower than non-supersonic; that on the conditionof UV-light ,in a word, it took negative effect to desorption The"reproduced process"has been used in the procedure of regeneration.

实验结果表明，初始阶段，三价砷的脱附率高于五价砷，但最终脱附率三价砷低于五价砷；三价砷、五价砷在不同温度下的脱附实验表明三价砷的最佳脱附温度低于五价砷的最佳脱附温度；在pH=5.5-8.5时，随着pH的增大，五价砷的脱附率具有先减小再增大的趋势，在pH为6.5时脱附率降至最小；在pH=5.5-8.5时，三价砷的脱附率随pH的增大而增大；不论是三价砷还是五价砷的脱附过程，超声波条件都会促进脱附速率(来源：ecABC论a4文网www.abclunwen.com)的增加，并且最终的脱附率要高于无超声波条件下的脱附率；紫外光照条件下，随着光照时间的增加，两种价态砷的脱附率会达到一个较高的值，再降低。总体而言，紫外光照的存在不利于脱附的进行。

CTAC had the best effect, bringing the sludge cake moisture down to 68%. At 0℃, the above test was also done for the following conditions: untreated, CPAM, FeC13/CaO, and acid conditioning. The sludge cake moisture is lower at 0℃ than that at normal temperature. The untreated sludge and pH=2 sludge had the lowest moisture. At -15 ℃, CTAC brought the sludge cake moisture down to 62.8%, 6% lower than at normal temperature.

结果表明，CTAC对污泥的调理效果最好，可使污泥滤饼含水率降至68%。0℃时，未经处理、CPAM、FeCl3/CaO、加酸处理条件下污泥脱水效果较常温时都有不同程度的提高，效果最好的为未经处理和pH=2条件下的泥样；-15℃时，CTAC条件下滤饼含水率较常温下降了约6%，达到62.8%。

Multi-function string which combines hollow rod electric heating, adding hot waste water above pump, adding hot waste water below pump, hot water circulation below pump, using sinker bar below pump, and borehole chemical viscosity reduction has been used selectively according to different production requirement and reservoir conditions.

采用能实现空心杆电加热、泵上掺热污水、泵下掺热污水、泵下热水循环泵上掺入、泵下配置加重杆、井筒化学降粘等多功能综合管柱，适应各种不同生产要求，根据油层条件，选取一种或多种组合、配套。

But our Patron warn'd by this Disaster, resolved to take more Care of himself for the future; and having lying by him the Long-boat of our English Ship we had taken, he resolved he would not go a fishing any more without a Compass and some Provision; so he ordered the Carpenter of his Ship, who also was an English Slave, to build a little State-room or Cabin in the middle of the Long Boat, like that of a Barge, with a Place to stand behind it to steer and hale home the Main-sheet; and Room before for a hand or two to stand and work the Sails; she sail'd with that we call a Shoulder of Mutton Sail; and the Boom gib'd over the Top of the Cabbin, which lay very snug and low, and had in it Room for him to lye, with a Slave or two, and a Table to eat on, with some small Lockers to put in some Bottles of such Liquor as he thought fit to drink in; particularly his Bread, Rice and Coffee.

这次意外事件给了我们主人一个警告，他决定以后得小心谨慎一些，出海捕鱼时带上指南针和一些食品。正好在他俘获的我们那艘英国船上，有一只长舢舨。他就下令他船上的木匠--也是他的一个英国人奴隶--在长舢舨中间做一个小舱，像驳船上的小舱那样；舱后留了些空间，可以容一个人站在那里掌舵和拉下帆索；舱前也有一块地方，可容一两个人站在那里升帆或降帆。这长舢舨上所使用的帆叫三角帆，帆杆横垂在舱顶上。船舱做得很矮，但非常舒适，可容得下他和一两个奴隶在里面睡觉，还可摆下一张桌子吃饭；桌子里做了一些抽屉，里面放上几其他爱喝的酒，以及他的面包、大米和咖啡之类的食物和饮料。

Equivalent circuit is deduced, and then experiments on photovoltaic cell are relative...

最后对50WP多晶硅光伏电池在不同情况下的运行特性进行了实验研究，比较光伏电池在不同倾角下、不同负载及不同气温条件下对太阳能光伏发电系统发电效率的影响，实验数据确定最佳方位角和倾斜角，并分析了阴影对光伏电池的影响，数据表明当阴影遮盖面积超过50%后光伏电池输出功率降到原来的1%。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。