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METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉,依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪,I型胶原酶酶解法分离出脂肪源间充质干细胞(adipose-derived mesenchymal stem cells,ADSCs),进行体外传代后差速贴壁培养纯化。

Methods: Forty pregnant rats were randomly divided into 4 groups and were given DBP by gastric intubation at a dose of 0, 100, 500 or 800 mg/kg from the 14th day to the 18th day of pregnancy. The incidence of cryptorchidism was evaluated in each group 70 days after birth. The histological changes of testis and epididymis in cryptorchid rats were also observed.

孕鼠40只,随机分组,在怀孕14~18天期间,每天分别灌胃给予大豆油、DBP 100mg/kg、500mg/kg、800mg/kg,出生70天后观察雄仔鼠隐睾发生率及隐睾睾丸、附睾病理组织学改变。

The results showed that Eperythrozoon exited not only in blood, but also in liver, spleen, heart, lung, lymph nodes, brain, spinal cord, bone marrow, feces, testicle, epididymis, ascites, bile, cerebrospinal fluid, seminal fluid, milk, etc; there were a number of Eperythrozoons in liver, spleen, lung, milk.

研究发现附红细胞体不仅寄生于血液而且还寄生于肝、脾、心、肺、淋巴结、脑、脊髓、骨髓、粪便、睾丸、附睾、腹水、胆汁、脑脊液、精液、乳汁等组织和分泌物和体液中,而以肝、脾、肺、乳汁中数量最高。

It has been reported that gestational and lactational exposure to estrogens or androgen antagonists reduced testicular size and sperm production, germ cell apoptosis, male infertility, and male reproductive tract malformations, such as cryptorchidism, hypospadias, nipple retention, or reduced anogenital distance.

但本实验结果显示,大鼠孕期和哺乳期接触达母体中毒剂量的硫丹,对雄性子鼠的睾丸重量、每日精子生成量、附睾精子数和精子形态、睾丸生殖细胞凋亡发生率等都没有明显的影响,也未见雄性子鼠AGD缩短、乳头残留及隐睾和尿道下裂等雌性化表现。

Part I the expression of Mahoganoid protein and it's mRNA in the testes and epididymides of mature ratObjective To localize the Mahoganoid protein and Mahoganoid mRNA in the testes and epididymides of mature male rat.

第一部分Mahoganoid蛋白及其mRNA在成年大鼠睾丸和附睾中的表达目的探讨Md蛋白及其mRNA在雄性成鼠睾丸和附睾组织中的分布。

The rat epididymal epithelial cells were cultured in vitro and the changes in flagelium activity and linear motile ability of the sperm that had been co-cultured with epithelium from corpus-cauda epididymides were observed.

目的:对体外培养的大鼠附睾上皮细胞进行动态观察与鉴定,了解体外条件下精子与附睾上皮细胞共同培养后精子活动力的变化。

Maybe it is a new member of b-galactosidase family. bin2a mrna is upregulated by androgen and also epididymis-specific, majorly in caput epididymides.

所以,bin2a可能是b-半乳糖苷酶家族的新成员。bin2a表达受雄激素上调,其表达的组织专一性也很高,只在大鼠附睾有表达,并主要在附睾头部。

Bin2a mRNA is upregulated by androgen and also epididymis-specific, majorly in caput epididymides.

Bin2a表达受雄激素上调,其表达的组织专一性也很高,只在大鼠附睾有表达,并主要在附睾头部。

Sperm of Epididymides and ejaculation could bind tetramethylrhodamine-6-dUTP labeled exogeous DNA.

本实验还对附睾精子和射精精子结合外源DNA情况的比较,发现附睾精子与射精的精液经洗涤后的精子都能够吸附外源DNA,且二者的差异不显著。

In order to accurately identify the sites of action of oestrogens and androgens, we have proceeded to the immunohistochemistry localization of the oestrogen receptors and the androgen receptor in the reproductive system and adrenal of sheep through gestation to adult.

本研究的包括下列四方面主要研究内容和研究结果:一、首次发现70d、90d、120d的小尾寒羊胎儿和新生羔睾丸、附睾和输出小管中没有ERβ表达,在输出小管上皮细胞中有大量的ERα,睾丸和附睾中没有ERα。

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