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阿霉素

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In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株(KB-A-1)的多药抗药性问题,本文从反义核酸和反基因核酸角度,通过MTT法检测细胞生长情况,ELISA法检测基因表达产物P-gp表达水平的变化,对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

Heat treatment can apparently confronte multidrug-resistant of 7721/Adm and increase the sensitivity of 7721/Adm to ADM.,and the resulet indicate that increasing the sensitivity can be related to intracellular concentration of ADM.

结论加热可以显著对抗7721/Adm的耐药性,提高其对阿霉素的敏感性,这与加热提高了细胞内药物浓度有关。

This study aims at the expression of Wnt signaling pathway inhibitor FrpHE and DKK-1 induced by ectogenous factor.

本课题的目的在于研究外源性因素对Wnt通路抑制剂FrpHE和DKK-1的诱导表达,我们选择抗癌药物p53及化疗药阿霉素、羟喜树碱、顺铂作为外源性诱导剂,以期弄清Wnt通路及其抑制剂FrpHE和DKK-1是否能够被p53和化疗药这两类药物所调节。

The endocellular Fura-2 increased to 2.39,3.14,and 3.24 fold respectively by addition of AA,bullatacin and atemoyacin-A and the accumulation of Dox in MCF-7/ADR cells was similar to that of Fura-2,increased to 2.98,4.10 and 4.24 fold respectively by AA,bullatacin,and atemoyacin-A while no in MCF-7 cells.

细胞内阿霉素的积累与Fura-2 相似,加入AA,bullatacin和atemoyacin-A均分别增加 MCF-7/ADR 细胞内 ADR 的积累至2.98,4.10和4.24倍,而未见显著增加敏感株内 ADR 的积累。bullatacin和atemoyacin-A均能诱导MCF-7与MCF-7/ADR细胞的凋亡,且相同浓度下,两细胞株的凋亡百分数无显著差异。

Methods:Eightythree patients with galactophore cancer were stochastic divides into observation group and contrast group.

83 例乳腺癌患者,随机分成观察组和对照组。观察组43 例,接受以ADM为主的CAF方案(环磷酰胺+阿霉素+氟尿嘧啶)。

Methods 1% adriamycin was injected in the infraorbital branch of the trigeminal nerve of 28 cases underwent trigeminal neuralgia of the second branch.

对28例原发性三叉神经痛第Ⅱ支的患者用1%阿霉素对眶下神经干进行多点多层次注射。

The rats' infraorbital nervous tracts were exposed during test. 0.5% Adriamycin was directly injected into the axons, while normal saline into the opposite site as control.

本实验通过手术解剖暴露大鼠眶下神经束,用0.5%阿霉素直接神经干内注射,对侧用生理盐水注射作自身阴性对照。

Methods: By using JSB-1 with intramembranous antigen determinant as antibody, the positive rate as optimization index and MCF-7/A as model cells, cell pretreatment methods, such as the presence or Absence of FACS permeabilizing solution, the concentration of FACS permeabilizing solution, the time treating cells with FACS permeabilizing solution, the time incubating cells with JSB-1 working fluid, the time incubating cells with goat-anti-mouse IgG-FITC solution, and the conditions for keeping JSB-1 working fluid and cell numbers, were optimized.

采用抗原决定簇在细胞膜内的单抗JSB-1,以阳性百分比为优化指标,耐阿霉素人乳腺癌细胞系MCF-7/A为模型细胞,对FACS透化溶液的有无、FACS透化溶液的浓度、FACS透化溶液处理细胞的时间、鼠抗JSB-1抗体的孵育时间、羊抗鼠IgG-FITC抗体的孵育时间、JSB-1抗体工作液的保存条件、以及细胞数量进行优化。

Methods 36 patients with mastocarcinoma Ⅲ are given CAP regimen (Cyclophosphamide、 Adriamycin, arid Cisplatin) for one cycle-of 21 - 28 days,after preoperation chemotherapy 2-3 cycles,evaluation of effect is conducted.

在我院收治的36例Ⅲ期乳腺癌患者术前应用环磷酰胺联合阿霉素和顺铂方案21~28天为一周期,术前化疗2~3周期后评价疗效。

OBJECTIVE: To observe the in vivo location and differentiation of 4',6-diamidino-2-phenylindole and green fluorescence protein-labeled cells in adriamycin nephrosis rats so as to explore an efficient labeling and tracing method for metanephric mesenchymal cells derived from embryonic rats.

目的:观察DAPI及绿色荧光蛋白标记细胞在阿霉素肾病大鼠体内的定位及分化,探讨便捷的大鼠胚胎后肾间充质细胞的体外标记方法。

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