阻滞药

This is most effective if a specific receptor blocking agent is available to counter the effects of a given agonist .

在这方面最有效的是利用特效的受体阻滞药对抗特定激剂的作用。

This is most effective if a specific receptor blocking agent is available to counter the effects of a given agonist.

在这方面最有效的是利用特效的受体阻滞药对抗特定激剂的作用。n.1。计算者；计算器；计数器

Anesthesis was performed by interscalene brachial plexus block.the onset and duration of analgesia,the level of block as well as adverse effects were recorded.hr and map were measured before anesthesia,5,10,30 and 60 minates after anesthesia.results:the onset time of block was significantly shorter and shivering was significantly lower in groups two and three as compared with group one.there was significant difference in the duration of anesthesia between group two and group three.

肌间沟法行臂丛神经阻滞，观察阻滞起效时间及持续时间、神经阻滞节断数、术中镇痛质量、不良反应以及注药前、注药后5、10、30、60分时心率和平均动脉压。结果：二、三组麻醉起效时间短于一组（p＜0.05）；麻醉持续时间二、三组长于一组但差异无显著性；二、三组组间比较差异有显著性；各组阻滞节断数、术中牵涉痛发生率、hr及map差异无显著性；一组寒战发生率高于二、三组（p＜0.05）。

Group one received 0.375% bupivacaine;Group two 2.5% chloroprocaine;Group three in which 1:200 000 adrenaline was added to 2.5% chloroprocaine. Anesthesis was performed by interscalene brachial plexus block.The onset and duration of analgesia,the level of block as well as adverse effects were recorded.

肌间沟法行臂丛神经阻滞，观察阻滞起效时间及持续时间、神经阻滞节断数、术中镇痛质量、不良反应以及注药前、注药后5、10、30、60分时心率和平均动脉压。

Methods］By observing the cytoplasmic Ca2+ concentration change of single cardiocyte in I/R status and the effect of Na+/H+ permutoid retarder to Ca2+ concentration in different phases (diabetes pure oxygen-lack deficit group called DM group,the oxygen deficit/reaeration on entire journey for medicine group called DM-EIPA group, the first group giringmedicine before reaeration on called DM-EIPA 1 group, and the second group giringmedicine before reaeration on called DM-EIPA 2 group) to detect the protection mechanism of Na+/H+ permutoid retarder to diabetic mouse cardiac muscle cell oxygen deficit/reaeration injury.

方法］ 通过观察糖尿病鼠单个心肌细胞在缺氧/复氧时细胞胞浆Ca2+浓度的动态变化以及Na+/H+交换体阻滞剂在不同时相（糖尿病单纯缺氧组即DM组，缺氧/复氧全程给药组即DM-EIPA组，复氧前给药1组即DM-EIPA 1组，复氧前给药2组即DM-EIPA 2组）对Ca2+浓度的影响来研究Na+/H+交换体阻滞剂对糖尿病鼠心肌细胞缺氧/复氧损伤的保护机制。

Twenty-five percent of Shen Feng Jing started its retardance on Indian bullfrog's nerve conduction in 4 min after giving medicine while only 2 min for 50%( P .05), and the retardative effect strengthened with the time.

25％ 的神蜂精溶液在给药后4m in 对蛙坐骨神经动作电位的传导开始有明显的阻滞作用（P＜ 0.05）； 50％以上神蜂精溶液在2m in 内就开始有显著阻滞作用（P＜ 0.05），并随给药时间推移，阻滞作用不断加强，表现出明显的时程效应。

Background— In a recent study, we demonstrated that an increase in oxidatie stress in the rostral entrolateral medulla plays a critical role in the sympathoexcitation obsered in chronic heart failure.

背景：在最近的一次研究中，我们发现在交感神经阻滞药治疗慢性心力衰竭作用中延髓头端腹外侧氧化应激增强发挥关键作用。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

We already put him on beta blockers and nitroglycerine,no effect.

我们已经给他注射了β-受体阻滞药和硝化甘油没有效果

If, in somehideous reincarnation, I found myself the prime minister or a CEO,I dare say I'd be on uppers and downers and beta-blockers andsleeping pills to make things seem less bad.

如果，在某次可怕的转世投胎中，我成了首相或首席执行官，那么我敢说，我会依靠兴奋剂、镇静剂、β受体阻滞药和安眠药，来使事情看上去不那么糟糕。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。