阻滞

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

At the BCL of 500ms, 1000ms and 2000ms,the VWtimes increased markedly with the BCL prolonging, and the degree of increasing was even obviously when heart rate was slow (BCL=2000ms or 1000ms). The enlargement of VWs was a most important reason to increase the incidence of ventricular arrhythmias; 2）At the BCL of 500ms, 1000ms or 2000ms,the VWtimes of the premature stimulates S2 was at endomembrane were wider than at the outermembrane. We supposed that premature stimulates come from endomembrane was easier inducing VF compared with outermembrane;3The VWtime of unidirectional block was wider than reentry's, we can see that reporlarization dispersion changed dynamicly. 4The QRS polar of the premature stimulates was opposite the primary beats ,which suggesting that excitation of inducing premature beats was coming from epicardial. That is to say electrotonic potential take part in the creation of reentry;5With the ratio of the Tp-e/QT increasing the occurrence of VF was upgrading. So the ratio was an effective method to estimate the event of SCD.6 The VWtimes of S3 were broaden than S2's. But we can not study penetratingly, which is the contents of our future studies.

在BCL为500ms、 1000ms和2000ms刺激条件下，随着刺激周长的增加单向传导阻滞时间易损窗呈增大趋势；并且在心率较慢时(BCL为2000ms和1000ms)增大最明显，是LQTS患者室性心律失常发生率增加的原因之一；2）在BCL为500ms、 1000ms和2000ms刺激条件下，S2位于内膜侧时时间易损窗大于在外膜侧时的，推测来自于内膜侧的期前刺激易于诱发室性心律失常；3）折返激动时间易损窗小于在单向传导阻滞时间易损窗，折返易发生于S2刚进入单向传导阻滞区间时，这说明复极梯度的不均一是动态变化的；4心电图上倒置QRS波与正常S1刺激所引起QRS波极性相反，提示不同位置S2刺激引发的室性早搏的兴奋均来自于外膜侧，说明了电紧张电位扩布参与了折返的产生；5）Tp－e/QT可以反应跨膜复极离散度，随着Tp－e/QT比值的增加PVT的发生增多，其可以用来预测室性心律失常的发生。6）随着期前刺激数目的增加时间易损窗增大。

The two other main branches of the dorsal ramus are the intermediate branch, which sends fibers into the longissimus muscle,and the lateral branch, which innervates the iliocostalis muscle, the thoracolumbar fascia, the skin of the lower back and buttock, and the sacroiliac joint.29,136,137 At the superomedial border of the trans-verse processes where the lumbar medial branches are most amenable to blockade, these nerves are in such close proximity that anesthetizing any one of them will almost invariably lead to blockade of the others branches of the primary dorsal rami.

背侧支另外的两个分支是支配最长肌的中间支及支配髂肋肌、胸腰筋膜和下腰部皮肤，臀部及骶髂关节的外侧支。在横突上内侧缘，内侧支容易被阻滞，在此处的神经彼此距离很近，阻滞一支即可将其他背侧支一齐阻滞。

Therefore, for this prospective randomized study, we recruited72 patients scheduled for hand or forearm surgery and comparedthe speed of execution and quality of USG infraclavicular blockwith either USG alone or USG combined with neurostimulation.

因此，在这项前瞻性随机研究中，我们选取了72例计划行手或前臂手术的患者，比较 USG 行锁骨下神经阻滞时，单独应用 USG和 USG 联合神经刺激神经阻滞术完成的速度和阻滞效果。

When the cells were exposed to 10 nmol/L taxol for 24 h,significant cell-cycle arrest was also observed by fluorescence microscope,coexisting multinucleated cells were found as well.

随着作用时间延长，〓期阻滞增强，凋亡细胞百分率也随之增高，凋亡的出现滞后于有丝分裂阻滞，其峰值亦滞后于细胞周期阻滞的峰值。

Sensory block, motor block, and supplementation rateswere evaluated for the musculocutaneous, median, radial, andulnar nerves.

感觉阻滞，运动阻滞和添药率评估肌皮神经、正中、桡神经、尺神经等阻滞情况。

Junction rhythm block in four patients, second-degree heart block in one, and right bundle branch block in four patients.

9例(8%)患者并发其他心律失常：包括4例节律阻滞，1例II度房室传导阻滞，4例右束支传导阻滞。

Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC 7) recommends treating most patients with stage 2 hypertension (160/100 mm Hg or higher) with a two-drug combination of a thiazide diuretic plus an ACE inhibitor, angiotensin receptor blocker, beta blocker, or calcium channel blocker.

美国国家联合委员会关于高血压预防、诊断、评估与治疗第七次报告（JNC 7）建议，大多数2期高血压（≥160/100 mm Hg）患者应接受一种噻嗪类利尿剂加一种血管紧张素转换酶抑制剂、血管紧张素受体阻滞剂、β受体阻滞剂或钙通道阻滞剂的两药联合方案治疗。

METHODS: Sixty patients undergoing elective unilateral TKAunder spinal anesthesia received in a randomized, double-blindmanner a femoral, obturator, or sham nerve block at the endof surgery.

60名在脊麻下择期行单侧膝关节成形术的患者，在手术结束后接受随机双盲的股神经阻滞或闭孔神经阻滞或未作神经阻滞的临床研究。

Results Among the ECG of old ophthalmic patients,right bundle branch block (25.8%)was the most common one,followed by sinus bradycardia(25.1%),supraventricular premature complexes(13.7%),sinus tachycardia (12.1%),ventricular premature compl exes(9.5%),sinus arrhythmia(7.9%) left bundle branch block(7.4%),AV block(5.9%), atrial fibrillation(5.4%).

结果：老年眼科疾病患者心律失常中右束支传导阻滞最多见(25.8%)，其次为窦性心动过缓(25.1％)，室上性早搏(13.7%)，窦性心动过速(12.1％)，室性早搏132例(9.5%)，窦性心律不齐(7.9%)，左束支传导阻滞(7.4%)，房室传导阻滞(5.9%)，房颤(5.4%)。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。