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Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化;RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化;抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达,包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases, ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor, Rho-GDI alpha),研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

The State will of course try to slow or halt the spread of this technology, citing national security concerns, use of the technology by drug dealers and tax evaders, and fears of societal disintegration.

国家当然会试图阻碍或者阻断这种技术的传播,他们会提出国家安全方面的理由,比如,毒贩或者逃税的人会使用这种技术,或者,他们会提出说社会可能因此而分崩离析。

The surgical excisions of hepatocellular carcinomas are safe and feasible only if the liver function reserve could be judged accurately before operation and the intraoperative hemorrhage and blood transfusion could be controlled effectively and the duration of portal clamping could be shortened during the operation.

降低肝癌切除术后并发症发生率的关键在于术中有效地控制出血及输血量,同时对伴存肝硬化的病人应尽量缩短肝门阻断时间。

This rapid inhibitory effect has also a larger calcium-dependent exocytosis reduction effect.

在细胞外液加入BIS,或用PTX孵育细胞24小时,可将皮质酮的抑制作用阻断

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素(bleomycin,BLM)复制大鼠肺纤维化模型,并分离培养肺成纤维细胞,用阿托伐他汀对其进行干预,分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组,用倒置纤维镜观察细胞的形态结构,MTT计数法观察各组细胞的增殖情况,免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ,α-SMA)的表达情况。

Although we have not a tool to directly modulate the cellular Cdc42/Rac activity at present, nevertheless, from the abolish of BDNF-triggered attraction by either DN-Cdc42 and CA-Cdc42, we propose that an intracellular Cdc42 activity gradient may also be sufficient to determine the turning direction, say, filopodia extend preferentially at the higher Cdc42 activity side, and the growth cone turn towards this direction in the hence.

虽然我们目前还没有一个直接操纵细胞内 Cdc42或其下游分子活性的方法,但从 DN-Cdc42和 CA-Cdc42均阻断到 BDNF 和 ryanodine 的吸引作用来看,我们推测, Cdc42的活性梯度很可能也能够决定生长锥转动方向,即丝状伪足在 Cdc42活性高的一侧优先延伸,生长锥朝向该侧转动。

To avoid loss of drilling tools and even well abandonment due to downhole fire or explosion during air drilling in pay zones, we developed a novel downhole fire or explosion prevention sub, a kind of downhole tool made of low melting-point alloy, designed to shield off oxygen so as to put out fires. This sub is connected to the upper part of dill bit.

为了解决空气钻井在钻遇油气层时,发生井下燃爆,造成埋钻具、井眼报废等复杂情况,基于阻断氧气使火熄灭的思路研制出了一种低熔点合金制造的井下工具-空气钻井燃爆灭火阀。

To avoid loss of drilling tools and even well abandonment due to downhole fire or explosion during air drilling in pay zones, we developed a novel downhole fire or explosion prevention sub, a kind of downhole tool made of low meltingpoint alloy, designed to shield off oxygen so as to put out fires. This sub is connected to the upper part of dill bit.

为了解决空气钻井在钻遇油气层时,发生井下燃爆,造成埋钻具、井眼报废等复杂情况,基于阻断氧气使火熄灭的思路研制出了一种低熔点合金制造的井下工具--空气钻井燃爆灭火阀。

If a small fire blocks the emergency exit , you'd better rush out of the exit decisively by soaking

小火阻断安全出口时,要果断地披上浸湿的衣服、被褥等物冲出去。

Blocked flues and chimneys stop the gas escaping this allows it to build up in a room where it could be breathed in.

阻断火道及烟囱停止气体逸出这使得它建立在一个房间里,它可吸入英寸

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。