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This review focuses on newly developed strategies for miRNA quantification, and elucidates in detail the probe-hybridization based methods including Northern blotting, microarray, gold nanoparticle labelling, and splinted ligation with radioactive labels. The amplification-based methods including quantitative PCR, rolling cycle amplification, invader assay, and the next generation sequencing methods were also discussed. The advantages and disadvantages of these methods were compared.

文章系统地介绍了最新发展的microRNA定量检测方法,详细阐述了基于探针杂交技术的Northern blotting法、微阵列芯片法、纳米金标记法、桥连同位素标记法,以及基于扩增技术的定量PCR检测法、滚环扩增法、引物入侵法和新一代大规模高通量测序法等,并对这些方法的优缺点进行了分析比较。

This opens the Array dialog box, where you can quickly create the other wheel spokes.

这将打开阵列对话框,在这里您可以快速创建其他车轮辐条。

Adaptive array antennas have become one of the spotlights of the Third Generation mobile communication.

自适应阵列天线已经成为第三代移动通信研究的热点之一。

Three-side teethed feather-like nanocomb structures of ZnO were produced based on a vapor-phase transport process.

样品的几何形状表现为一根主干和沿着主干三个对称方向上有纳米齿状阵列

Triples said input and output for the usual array of forms.

输入用三元组表示,输出为通常的阵列形式。

Through the study of water tank experiment, obtain that the Channel unitizing Toeplitz error calibration method is effective. It can improve the performance of algorithms under array errors obviously, has great prospect in engineering.

通过水池实验的研究表明:通道归一化Toeplitz的校正方法最为有效,可以大幅度改善阵列误差对阵元域及波束域高分辨算法带来的影响,是一种有工程应用价值的方法。

Re- versing true formation resistivity through array induction logging technology can solve the pro.

采用阵列感应测井技术反演地层真电阻率有效解决了咸水泥浆侵入造成电阻率降低的问题,在辽河滩海地区收到了明显的地质应用效果。

In this paper we deal with the problem of timing synchronization of OFDM systems under array receivers.

本文主要研究了阵列接收机条件下的OFDM系统的同步问题。

An analytical method based on high performance liquid chromatography with diode array detector has been established for the simultaneous determination of 9 water-soluble colorants (Solvent Green 7, Food Yellow 3, Food Red 17, Acid Yellow 1, Acid Red 33, Food Red 4, Food Red 1, Orange I, Acid Orange 7) in cosmetics.

建立了用高效液相色谱-二极管阵列检测器同时检测化妆品中9种水溶性着色剂(溶剂绿7、食品黄3、食品红17、酸性黄1、酸性红33、食品红4、食品红1、橙黄I、酸性橙7)的检测方法。

After Scatchard analysis, the affinity constant of AR-LBD bound to testosterone was 5.32nmol/L.

通过Scatchard方程计算得到微阵列上AR—LBD与荧光标记的睾酮结合的Kd值为5.32nmol/L。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。