阴性的

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

objective:study female the yin false wet you of clinical pathology characteristics and discriminate judgment.method:rightness 69example sufferer of clinical and laboratory check the data carry on analysis.result:pcv and small labium seamy side the most familiar(91.3%) skin external appearance with floss form familiar(62.3%) be located on a roe of fish form a time it;(33.33%)organization pathology show the upper part in the ji layer to fill the air a sex net basket a form empty bubble cell kind change;the acetic acid experiment a rate;(97.17%)human milk head lump virus second type examination feminine gender.conclusion:pcvhave a clinical characteristics of show the zhao, acetic acid of experiment hpv examination have importance value towards discriminate analysis.

目的：探讨女阴假性湿疣的临床病理特点及鉴别判断。方法：对69例患者的临床及实验室检查资料进行分析。结果：女阴假性湿疣及小阴唇内侧最为常见(91.3％)皮肤外观以绒毛状常见(62.3％)位于鱼子状次之(33.33％)；组织病理示棘层中上部弥漫性网篮状空泡细胞样改变；醋酸的试验率(97.17％)；人乳头瘤病毒亚型检测阴性。结论：女阴假性湿疣具有显著的临床特点，醋酸的试验hpv检测对鉴别分析有重要价值。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The use of Rh immune globulin to prevent the disease in babies of Rh negative mothers has become standard practice, and the disease, which used to claim the lives of 10,000 babies each year in the US alone, has been virtually eradicated in the developed world. In 1980 Drs. Pollack, Gorman, Freda, and Finn shared the Albert Lasker Award for their work on Rh disease.

使用Rh免疫球蛋白来预防在Rh阴性血型的母亲的胎儿的疾病，成为一种常规做法，这种曾经仅在美国每年夺走10，000名婴儿生命的疾病，在发达国家几乎被摧毁了。1980年，博士波拉克，戈尔曼，弗里达，芬恩由于他们在Rh疾病上的工作分享了亚伯雷斯克奖。

Methods The recombinant sequence for two CEA epitopes was tandemly engaged and inserted into-upstream of in-frame-varied HSP70 of Mycobacterium tuberculosis to construct a gene vaccine.Balb/c mice were muscularly injected with recombinant DNA vaccine;negative control (mice were injected with normal saline), positive control (mice were injected with DNA vaccine harboring tandem CEA epitopes plus aluminium hydroxide adjuvant) and experimental group(mice were injected with PCITri CEA625-667-met HSP70) were set up.

在已经构建含有变异热休克蛋白序列的基础上，插入重组CEA串联表位的编码片段获得CEA串联表位-HSP融合基因疫苗。3次肌肉注射免疫Balb/c小鼠，设立注射生理盐水的阴性对照组、注射氢氧化铝佐剂混悬CEA串联表位的阳性对照组及注射pCITriCEA625-667-mtHSP70的实验组。

Essential oil was extracted from the petroleum ether-soluble fraction of the leaves showed having antibacterial activity. It was demonstrated that the essential oil has significant antibacterial activity and the activity of the petroleum ether-soluble fraction is attributed to it. The constituents of the essential oil were analysized by GC-MS.10 relatively abundant components were identified on the basis of MS, among which Geranlgeraniol was found in the essential oil of Perilla frutescens leaves for the first time.

对具有抗菌活性的紫苏叶石油醚萃取物进一步精制，从中提取出挥发油，通过抗菌测试证明紫苏叶挥发油具有良好的抗革兰氏阳性菌和革兰氏阴性菌活性，是紫苏叶石油醚萃取物抗菌活性的来源；采用GC-MS对紫苏叶挥发油进行了成分分析，得到10种含量较高的主要化合物，其中的牻牛儿基牻牛儿醇首次在该植物挥发油中被发现。

The complex however, was caused in the first place by the assimilation of the mother (in itself a normal and ubiquitous phenomenon) to the pre-existent, feminine side of an archetypal "male-female" pail of opposites, and secondly by an abnormal delay in detachin~ from the primordial image of the mother.

无论如何，这个情结第一次是由于吸收母亲作为预先的存在而引起的，作为"男－女"原型的阴性面，第二次则从由脱离母亲的原始影象的反常延迟引起。

The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.

检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性，最低可以检测到10个TCID50/100μl的病毒，说明该方法的有较好的特异性和敏感性。

One of the needs was a dosing experiment that could be used to determine the level of circulating Rh-positive cells in an Rh-negative pregnant female derived from her Rh-positive fetus.

一个必须做的是一个剂量实验，用来检测在怀孕的Rh阴性女性循环血液中的Rh阳性细胞的含量，当然，这些Rh阳性细胞来自于胎儿。

But just in case there is not enough by the time of the Olympics, the Red Cross says it has a registry of 400 Rh-negative donors who can be called upon to donate in case of an emergency.

但是万一到奥运会时还没有足够的血液，红十字会说，它有400名Rh阴性血的献血者的登记，发生紧急情况的时候可以叫这些人献血。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失