阴性的

All the LOH on 〓 and 〓 was observed in invasive ductal carcino- ma, carcinoma simplex, medulary carcinoma and scirrhous carcino- ma, no deletions at these sites were observed in any invasive lobular carcinoma and others. These results imply an etiological difference.P53 gene is a hot point gene in the occurrence and development of breast, cancer. PCR-SSCP analysis was performed to detect P53 gene point mutation in the region between exon 5 and 8, 5 of 12 (41. 6%) stage I breast cancer patients contain mutation of P53, 3 of 5 patients were accompanied by 〓 deletion. These results suggested that point mutation and allelic loss of P53 gene are two vi- tal genetic events in earlier stage of breast tumorigenesis.

我们还发现，〓和〓位点LOH均分布在乳腺浸润性导管癌、单纯癌、髓样癌及硬癌中，在浸润性小叶癌和某些特殊类型乳腺癌中全部为LOH阴性，上述位点LOH可能与某些组织学类型乳腺癌的发生有关。P53基因是乳腺癌形成过程中的一个热点基因，本研究对12例Ⅰ期乳腺癌组织标本中P53基因热点区域第5、6、7、8外显子点突变进行了测定，发现41.6％（5／12）的病人有P53基因一个或多个点突变，其中3例同时伴有〓位点LOH，表明P53基因点突变和等位基因缺失是发生在乳腺癌形成早期的一个重要遗传学事件。

Methods 224 patients with liver biopsy and a positive HBsAg were included in this study. The AUROC of AST、ALT、AST/ALT and APRI were identified by using receiver operating characteristic curves. Sensitivity, specificity, positive and negative predictive values, and Youden Index of APRI at optima cut-off were calculated.

调查224名乙肝表面抗原阳性的乙肝患者，利用ROC曲线分析AST、ALT、AST/ALT和APRI的面积，判断APRI的最优截断点并计算不同标准的灵敏度、特异度、阳性预测值、阴性预测值、Youden指数。

Helicobacter pylon, a kind of gram-negative bacteria which colonizes in the huanin stomach, has been identified as a major pathogen associated with the development of chronic gastritis and gastroduodenal ulcer disease as well as gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma. The infection rate of Hp is very high.

幽门螺杆菌（Helicobacter pylori，简称Hp）是定植于人胃部的一种革兰氏阴性菌，它与慢性胃炎、消化性溃疡、粘膜相关淋巴样组织性淋巴瘤、胃癌等疾病的发生密切相关，它的感染率高，并且因为人免疫系统无法根除而长期生存于人的胃部。

BRAF mutations were detected in 13 of the 26 nodal nevi, but in just 1 of the 26 adjacent controls 50% vs.

同时，我们给每个病例都用了与其匹配的邻近淋巴组织作为阴性对照。26例淋巴结色素痣中有13例检测到BRAF基因的突变，邻近对照的淋巴组织中只有1例检测到BRAF的突变

Methods According to the drinking water legislation in Europe threshold: 0.10mg/L NO2(superscript -, a rapid spot test method for nitrite in water was established by using an optimized composition of reagent mixture and a binary positive/negative response, conducted as follows: a tablet of a mixture of solid reagents containing sulfanilamide, N-(1-naphthyl) ethylene diamine dihydrochloride and sodium chloride was put on a spot test plate of Teflon mould, then, a drop of 30% HCl and a drop of water sample were added on the reagent material.

根据欧洲饮水法规亚硝酸盐限值(0.10mg/L)，使用一种优化的试剂混合物和二元的阳性－阴性反应，建立一种水中亚硝酸盐快速点滴检验法。结果将1片含磺胺、N-（1-萘）乙二胺二盐酸盐和氯化钠的固体试剂混合物放在聚四氟乙烯模的点滴检验板上。加1滴30%盐酸和1滴水样于试剂片上。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

For transgenic mice,98%~100% of metaphases and 85%~94% of interphases showed hybridization signal.For negative control mice,100% of metaphases and 95%~96% of interphases showed no hybridization signal.

阳性转基因小鼠98%~100%的中期分裂相，85%~94%的间期核出现杂交信号；阴性对照小鼠100%的中期分裂相、95%~96%的间期核未出现杂交信号。

Antibody responses to hepati tis C virus and its modes of transmission in dialysis patients. Nephron, 1991, 5 9: 2328 Pol S, Romeo R, Zins B et al. Hepa titis C virus RNA in anti-HCV p ositive hemodialyzed patients: significance and therapeutic implications.

本组资料中具有输血史的患者在HCV抗体阳性组中所占的比例明显大于在HCV抗体阴性组中所占的比例，似乎支持这一观点，但另一方面，仍有8例HCV抗体阳性患者未曾有过输血史，提示HD患者存在输血以外其它途径感染的可能［3］。

objective to study the infection and sensitivity to antimicrobial agents of staphylococcus of bacterial prostatitis patients.methods the results of bacteriology and drug susceptibility of 421 patients,who were suffered from prostatitis,were analysed retrospectively.results in the 421 succus prostaticus samples,278 strains pathogenic microorganisms of 15 genera were isolated from the 273 succus prostaticus samples,and the major pathogenic microorganisms was staphylococcus aureus(57.2%) and coagulase negative staphylocous（17.3%.the drug resistance rate of staphylococcus to penicilling,erythromycin and albomycin was 88%-97%,and it was 33%-50% to fluoroquinolones.the drug resistance rate of staphylococcus to amikacin,cefazolin,nemycin,rifampicin and cefoperazone was lower than 30%,and they all appeared susceptible to vancomycin.conclusion staphylococcus is the main germ of bacterial prostatitis and it appears high drug resistance to usual antibacterials.staphylococcus is susceptible to vancomycin,amikacin,cefazolin,neomycin,rifampicin and cefoperazone.

目的 探讨细菌性前列腺炎患者葡萄球菌的感染状况及抗菌药物敏感性。方法回顾分析421例前列腺炎患者标本的细菌学和药物敏感性试验结果。结果 421例前列腺液标本，273例分离出细菌，共分离出15个种别共计278株病原菌，其中金黄色葡萄球菌占57.2%，凝固酶阴性葡萄球菌占17.3%；葡萄球菌对青霉素g、红霉素、白霉素耐药率为88%～97%，对氟喹诺酮类耐药率为33%～50%，对丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮耐药率均0%，对万古霉素全部显示敏感。结论葡萄球菌是细菌性前列腺炎的最常见病原菌，对临床常用抗菌药物具有较高的耐药性，葡萄球菌对万古霉素、丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮较为敏感。

In 96 patients who had none of these clinical features, CT was normal in 93, yielding a negative predictive value of 97%. All three of the misclassified patients had no evidence of brain herniation after LP, and only one had a mild mass effect.

在96名没有上述临床特徵的患者中，有93名CT检查结果正常，得到阴性预测值为97%，三个不归类的患者，在进行腰穿后没有发生脑疝的证据，只有一个患者，有轻微的异常反应。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。