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The method was to set up a freezing time ladder of the mixture of competented cell and plasmid along with a negative sample to research under the same condition of other aspects;The results revealed that the transforming efficiency was the highest at the freezing time of 20 minutes;Compared with the efficiency of other freezing times,the 20 minutes freezin...

在保证其他条件一致的情况下,设置大肠杆菌感受态细胞和质粒混合物的冰浴时间梯度并建立阴性对照进行研究。结果:冰浴20 min时转化效率最高。结论:与其他冰浴时间相比,冰浴20 min时的转化效率最高,实验重复性好,成本低廉,而且操作简便,值得推广使用。

Methods Mice were treated with three different doses of extract of ginger:high dose (9.3 mL·kg-1), middle dose (4.7 mL·kg-1),and low dose (2.3 mL·kg-1). All mice were irradiated once with 2.0 Gy X-ray. At the same time, the negative group (treated with vegetable oil only) and positive one (irradiated as well as extract of ginger groups after treated with vegetable oil) were set up. The changes of activities of enzymes in testes of mice were observed.

分别以高剂量(9.3 mL·kg-1)、中剂量(4.7 mL·kg-1)和低剂量(2.3 mL·kg-1)的生姜提取物给小鼠灌胃,以剂量为2.0 Gy的X射线一次性全身照射,24 h后观察不同组小鼠睾丸酶活性的变化,同时设阴性对照组和阳性对照组(给予植物油后与生姜提取物各实验组同时照射)。

Aminoglycoside for gram-negative bacteria have good antibacterial activity; cefotaxime resistance rate of 70%, lincomycin, levofloxacin against Streptococcus pneumoniae have good antibacterial activity.

氨基糖甙类对革兰氏阴性杆菌有较好的抗菌活性;头孢噻肟的耐药率70%,林可霉素、左氧氟沙星对肺炎链球菌有较好的抗菌活性。

We evaluated15 children with negative clinical findings and a normal electroencephalogram as part of their follow-up for prior episodes of epilepsy,recording the BIS and EEG in a waking state, in the variousstages of sleep and on reawaking. For each stage, the mean value,the standard deviation, and the range of BIS values were calculated.

笔者选择了15名既往有癫痫发作史的儿童作随访,当前临床指标阴性且脑电图正常,记录清醒状态、各个睡眠阶段以及再醒时的 BIS 和 EEG ,计算每个阶段 BIS 的范围值、平均值和标准差。

In the 25 patients with specimens suggesting fibroadenoma, excisional biopsy confirmed the diagnosis in 23 patients but revealed phyllodes tumor in two patients, yielding a negative predictive value of 93%.

建议为纤维性瘤的25名患者中,在23个患者中进行切除的组织切片检查而确认诊断,但是其中二名患者为叶状瘤,得到93%的阴性预测值。

For many years, Surgeons have been adopting PND to manage possible occult cervical metastasis, although these patients' neck palpations are negative there are possibilities of cervical metastasis in the malignant tumor of the head and neck.

对那些虽然颈部触诊为阴性,但仍有较大颈淋巴结转移可能性的头颈部恶性肿瘤,临床医生多年来一直采用预防性颈清扫术来治疗可能存在的临床隐匿的颈淋巴结转移。

Its evergreen leaves of the four seasons could be used to build the northern winter hardy turf, flower beds, flower niche habitat, its high degree of shade tolerance could be used to build inter-city high-rise green, its white flowers neatly in line could be used in landscaping.

因其叶片四季常绿,可以用于北方构建冬季耐寒型草坪、花坛、花境;其高度的耐阴性,可以用于构建城市高楼间的绿地;其整齐一致的白色花朵可以用于园林绿化中景观配置。

In the positive group of the micrometastasis, the difference was significant statistically ( p 0. 01). There was significant difference of the recurrence rate between the two groups (p .01).ConclusionHie method of conjointing the intermission - continuous - section and CK20 immunohistochemistry technique may be valuable to re-estimate the LN metastasis status and stage of colorectal cancer and predict the prognosis.

1、间断切片法结合CK20免疫组织化学方法有助于检出Dukes B期大肠癌淋巴结微转移,增加每个淋巴结的切片数量有助于提高微转移的检出率; 2、Dukes B期大肠癌低分化及癌肿侵犯肠周径>3/4者易发生淋巴结微转移; 3、淋巴结微转移阳性组与阴性组的生存率与复发率有显著差异

The Canadian trial showed a negative effect for screening, and because of that it is being subjected to the most intense scrutiny.

加拿大进行的临床试验结果显示筛检的阴性结果,并因此受到非常严密的审查。

The expression of nestin protein which is the mark of neural stem cells had been found in SD rat organ of Corti on postnatal day 16 in aminoglycoside group, while no expression on the same day in normal group.

银染法不支持新细胞是小胶质细胞的假说;结合以往免疫组化(白细胞共同抗原LCA,CD45)研究的阴性结果,认为可以初步排除小胶质细胞的可能。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。