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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Results Compared with that in the NP tissues, the number of α-SMA positive cells increased significantly in BPH tissue, and the number of vimentin positive cells increased moderately in the stroma and prominently surrounding the acinus and in the basal layer. In BPH tissue, the myosin and ERα staining signal was lost in the stromal cells surrounding the acinus, and the positive staining cells gathered into bunches in the stroma far away from the acinus, while the positive cells were sporadically distributed in the NP tissue. The PCNA positive cells increased moderately in the stroma and increased significantly in the basal layer.

结果 与NP相比在BPH中,α-SMA阳性染色细胞显著增加;波形蛋白在间质中阳性染色细胞有所增加,在腺泡基底层及临近腺泡外层间质中阳性染色细胞明显增加;在临近腺泡外的数层间质细胞中肌球蛋白和ERα由部分阳性变为完全阴性染色,而在远离腺泡的间质中其阳性染色细胞由散在斑块状分布变为簇状密集排列;PCNA在间质中阳性染色细胞有所增加,在基底细胞层中阳性染色细胞显著增加。

It has potent antibacterial activity towards kinds of anaerobe and aerobe. Doripenem was launched in Sept.

多尼培南对厌氧或需氧的革兰氏阳性和革兰氏阴性细菌都有强大的抗菌活性,该产品于2005年9月首次在日本上市。

CML derived MSCs showed normal karyotype and ultrastructure, they did not express BCR/ABL gene.

CML来源的MSCBCR/ABL融合基因阴性,不具备体内和体外致瘤性。

Objective: To discuss the security for Rh-positive patients receiving Rh-negative renal grafts.

目的:探讨Rh阳性患者接受Rh阴性亲属活体供肾移植的安全性。

Blood of pregnant women are generally the husband for the O-blood for other blood type and Rh-negative pregnant women, the husband is Rh-positive cases, easily lead.

一般是孕妇血型为O型而丈夫血型为其他血型及孕妇为Rh阴性,丈夫为Rh阳性的情况下容易引起。

Group antigen possessed by Rh-positive people; if an Rh-negative person receives a ''.

血液'。''抗原;如果洛氏阴性人群接受洛氏阳性人群的输血就会导致溶''。

Possessed by Rh-positive people; if an Rh-negative person receives a ''.

血液'。''抗原;如果洛氏阴性人群接受洛氏阳性人群的输血就''。

Results:tertiary institutions and stable job and the crowd is the ABO blood group Rh-negative blood type and focus of the main source of strokes screen,screening targets.

结果 大专院校和固定职业人群是ABO血型和Rh阴性血型的主要来源和重点招幕、筛查对象。

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